Investigation of Water Quality and Molecular Analysis (16s rRNA) of Bacterial Community Structure in a Rural Geothermal Spring: Ucler Hill (Azat Village), Kars, Türkiye

Orhan Uluçay

Makale detayları

Investigation of Water Quality and Molecular Analysis (16s rRNA) of Bacterial Community Structure in a Rural Geothermal Spring: Ucler Hill (Azat Village), Kars, Türkiye

Yazarlar:

Orhan Uluçay

Yayıncı:

Bingol University

Ana Başlıklar:

Biyomühendislik (Diğer)

Dergi:

Türk Doğa ve Fen Dergisi Vol.14 Issue 3, pp.218-225

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359154

pISSN:

2149-6366

Anahtar kelimeler:

Özet:

 This study was carried out to evaluate the chemical and microbiological properties of a natural water source located in Azat Village of Kars province and used as drinking water by the surrounding villagers. Water samples taken from three different sampling points were analyzed for pH, electrical conductivity, total hardness, alkalinity, nitrogenous compounds NO₂-, NO₃-, NH₄, metal contents Fe, Cu, Al, Pb, sulfate and chloride levels. The data obtained were compared with World Health Organization WHO drinking water standards and one-sample t-test was applied for each parameter. Statistically significant differences were found in total hardness, NO₃-, Fe, Cu, SO₄²- and Cl- parameters p  0.05, while other parameters were found to be compatible with WHO reference values. In microbiological analyses, a total of eight different bacterial species were isolated by culture-dependent methods. These species were identified by 16S rRNA gene sequencing analysis and all of them were determined to be non-pathogenic environmental microorganisms. The identified species included Microbacterium spp., Bacillus spp., Corynebacterium spp., Lactobacillus spp., Rhodococcus spp., Pseudomonas spp., Leuconostoc spp. and Aeromonas spp. Although some species have the potential to be opportunistic pathogens, the overall microbiological profile was considered to be within acceptable limits for natural sources.

 This study was carried out to evaluate the chemical and microbiological properties of a natural water source located in Azat Village of Kars province and used as drinking water by the surrounding villagers. Water samples taken from three different sampling points were analyzed for pH, electrical conductivity, total hardness, alkalinity, nitrogenous compounds NO₂-, NO₃-, NH₄, metal contents Fe, Cu, Al, Pb, sulfate and chloride levels. The data obtained were compared with World Health Organization WHO drinking water standards and one-sample t-test was applied for each parameter. Statistically significant differences were found in total hardness, NO₃-, Fe, Cu, SO₄²- and Cl- parameters p  0.05, while other parameters were found to be compatible with WHO reference values. In microbiological analyses, a total of eight different bacterial species were isolated by culture-dependent methods. These species were identified by 16S rRNA gene sequencing analysis and all of them were determined to be non-pathogenic environmental microorganisms. The identified species included Microbacterium spp., Bacillus spp., Corynebacterium spp., Lactobacillus spp., Rhodococcus spp., Pseudomonas spp., Leuconostoc spp. and Aeromonas spp. Although some species have the potential to be opportunistic pathogens, the overall microbiological profile was considered to be within acceptable limits for natural sources.

 This study was carried out to evaluate the chemical and microbiological properties of a natural water source located in Azat Village of Kars province and used as drinking water by the surrounding villagers. Water samples taken from three different sampling points were analyzed for pH, electrical conductivity, total hardness, alkalinity, nitrogenous compounds NO₂-, NO₃-, NH₄, metal contents Fe, Cu, Al, Pb, sulfate and chloride levels. The data obtained were compared with World Health Organization WHO drinking water standards and one-sample t-test was applied for each parameter. Statistically significant differences were found in total hardness, NO₃-, Fe, Cu, SO₄²- and Cl- parameters p  0.05, while other parameters were found to be compatible with WHO reference values. In microbiological analyses, a total of eight different bacterial species were isolated by culture-dependent methods. These species were identified by 16S rRNA gene sequencing analysis and all of them were determined to be non-pathogenic environmental microorganisms. The identified species included Microbacterium spp., Bacillus spp., Corynebacterium spp., Lactobacillus spp., Rhodococcus spp., Pseudomonas spp., Leuconostoc spp. and Aeromonas spp. Although some species have the potential to be opportunistic pathogens, the overall microbiological profile was considered to be within acceptable limits for natural sources.

 Bu çalışma, Kars ilinin Azat Köyünde bulunan ve çevre köylüler tarafından içme suyu olarak kullanılan doğal bir su kaynağının kimyasal ve mikrobiyolojik özelliklerini değerlendirmek amacıyla gerçekleştirilmiştir. Üç farklı örnekleme noktasından alınan su örnekleri pH, elektriksel iletkenlik, toplam sertlik, alkalinite, azotlu bileşikler NO₂-, NO₃-, NH₄, metal içerikleri Fe, Cu, Al, Pb, sülfat ve klorür seviyeleri açısından analiz edilmiştir. Elde edilen veriler Dünya Sağlık Örgütü WHO içme suyu standartları ile karşılaştırılmış ve her parametre için tek örneklem t-testi uygulanmıştır. Toplam sertlik, NO₃-, Fe, Cu, SO₄²- ve Cl- parametrelerinde istatistiksel olarak anlamlı farklılıklar bulunurken p0.05, diğer parametreler WHO referans değerleriyle uyumlu bulunmuştur. Mikrobiyolojik analizlerde, kültüre bağlı yöntemlerle toplam sekiz farklı bakteri türü izole edilmiştir. Bu türler 16S rRNA gen dizileme analizi ile tanımlanmış ve hepsinin patojen olmayan çevresel mikroorganizmalar olduğu belirlenmiştir. Tanımlanan türler arasında Microbacterium spp., Bacillus spp., Corynebacterium spp., Lactobacillus spp., Rhodococcus spp., Pseudomonas spp., Leuconostoc spp. ve Aeromonas spp. yer almaktadır. Bazı türler fırsatçı patojen olma potansiyeline sahip olsa da, genel mikrobiyolojik profilin doğal kaynaklar için kabul edilebilir sınırlar içinde olduğu düşünülmüştür.

Makale detayları

Investigation of Water Quality and Molecular Analysis (16s rRNA) of Bacterial Community Structure in a Rural Geothermal Spring: Ucler Hill (Azat Village), Kars, Türkiye

Yazarlar:

Orhan Uluçay

Yayıncı:

Bingol University

Ana Başlıklar:

Biyomühendislik (Diğer)

Dergi:

Türk Doğa ve Fen Dergisi Vol.14 Issue 3, pp.218-225

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359154

pISSN:

2149-6366

: Natural drinking water source

Microbial diversity

Rural water security

Chemical water quality

16S rRNA sequence analysisDoğal içme suyu kaynağı

Mikrobiyal çeşitlilik

Kırsal su güvenliği

Kimyasal su kalitesi

16S rRNA dizi analizi

Sayı

Makale detayları

Yazarlar: Orhan Uluçay

Yayıncı: Bingol University

Ana Başlıklar: Biyomühendislik (Diğer)

Dergi: Türk Doğa ve Fen Dergisi Vol.14 Issue 3, pp.218-225

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359154

pISSN: 2149-6366

Sayı

Yazarlar:

Orhan Uluçay

Yayıncı:

Bingol University

Ana Başlıklar:

Biyomühendislik (Diğer)

Dergi:

Türk Doğa ve Fen Dergisi Vol.14 Issue 3, pp.218-225

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359154

pISSN:

2149-6366