A Facile HPLC-PDA Method for Simultaneous Determination of Paracetamol, Methyl Paraben, Sunset Yellow and Carmosine in Oral Suspensions

Şule DİNÇ ZOR; Özlem AKSU DÖNMEZ

doi:https://doi.org/10.18596/jotcsa.403497

Makale detayları

A Facile HPLC-PDA Method for Simultaneous Determination of Paracetamol, Methyl Paraben, Sunset Yellow and Carmosine in Oral Suspensions

Yazarlar:

Şule DİNÇ ZOR | Özlem AKSU DÖNMEZ

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Kimya mühendisliği

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry 2018 Vol.5 Issue 2, pp.763-774

Belge Tipi:

Makale

Yayın Tarihi:

12/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000503226

Sıklık:

Üç Aylık

eISSN:

2149-0120

DOI:

https://doi.org/10.18596/jotcsa.403497

Özet:

 


In the present study, a simple, fast
and accurate HPLC-PDA method was developed for the simultaneous determination
of paracetamol (PAR), methylparaben (MP), sunset yellow (SSY) and carmoisine
(CAR) in oral suspensions. The concentrations of colorants are less than with
respect to those of active ingredient and this variation makes process of
analysis toublesome. In the developed HPLC method, efficient chromatographic
separation was achieved using reversed phase C18 column (4.6 mm x 150 mm x 5 μm
particle size) and phosphate buffer solution (pH = 6.5)-acetonitrile mobile
phase with a flow rate of 1.6 mL/min in the gradient mode. The eluents were
monitored via a PDA detector at 300, 254 and 230 nm. The mean retention times of
SSY, PAR, CAR and MP were found to be 1.58, 2.15, 3.81 and 4.42, respectively.
The proposed method was validated in accordance with ICH guidelines and it was
seen that the method met all requirements in terms of linearity, precision,
accuracy and selectivity. The developed method was successfully applied for
simultaneous determination of the studied compounds in two commercial oral
suspension samples. 


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Makale detayları

A Facile HPLC-PDA Method for Simultaneous Determination of Paracetamol, Methyl Paraben, Sunset Yellow and Carmosine in Oral Suspensions

Yazarlar: Şule DİNÇ ZOR | Özlem AKSU DÖNMEZ

Yayıncı: Turkish Chemical Society

Ana Başlıklar: Kimya mühendisliği

Dergi: Journal of the Turkish Chemical Society Section A: Chemistry 2018 Vol.5 Issue 2, pp.763-774

Belge Tipi: Makale

Yayın Tarihi: 12/2017

Belge Dili: İngilizce

Document ID (Org No.): 2000503226

Sıklık: Üç Aylık

eISSN: 2149-0120

DOI: https://doi.org/10.18596/jotcsa.403497

Sayı

Yazarlar:

Şule DİNÇ ZOR | Özlem AKSU DÖNMEZ

Yayıncı:

Turkish Chemical Society

Ana Başlıklar:

Kimya mühendisliği

Dergi:

Journal of the Turkish Chemical Society Section A: Chemistry 2018 Vol.5 Issue 2, pp.763-774

Belge Tipi:

Makale

Yayın Tarihi:

12/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000503226

Sıklık:

Üç Aylık

eISSN:

2149-0120

DOI:

https://doi.org/10.18596/jotcsa.403497