Determination of the Effectiveness of Metarhizium anisopliae, Trichoderma hamatum, and Diatomoceaus Earth, Applied Alone and in Combination against Callosobruchus maculatus F. (Coleoptera: Bruchidae)

Mehmet Yıldırım; Celal Tuncer; İsmail Oğuz Özdemir

Makale detayları

Determination of the Effectiveness of Metarhizium anisopliae, Trichoderma hamatum, and Diatomoceaus Earth, Applied Alone and in Combination against Callosobruchus maculatus F. (Coleoptera: Bruchidae)

Yazarlar:

Mehmet Yıldırım | Celal Tuncer | İsmail Oğuz Özdemir

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Tarımda Entomoloji

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.39 Issue 1, pp.195-206

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001312183

pISSN:

1308-8750

Anahtar kelimeler:

Özet:

 In this study, the efficiency of diatomaceous earth DT, Metarhizium anisopliae Ma, and Trichoderma hamatum Th isolates against Callasobruchus maculatus Coleoptera: Bruchidae adults was investigated under laboratory conditions. While Ma and Th isolates were used at three different concentrations 1  104, 1  106 and 1  108 sporesmL, the highest doses of each fungus 1  108 sporesmL were used in combination with three different doses of DT 200, 400, and 800 mgkg. The trials were conducted at 25C, 70 relative humidity, and 16:8 h light-to-dark periods. Individual applications exhibited 87.5 and 52.5 mortality on the eighth day after application for the highest doses of Ma and Th 1  108 sporesmL, respectively, and 100 mortality on the seventh day after application for the highest dose of DT 800 mgkg. Also, it was observed that mortality rates increase depending on time and dose. In combination trials, the most effective combination was DT 800 mgkg  Ma 1  108 sporesmL with a 100 mortality rate at the end of the fifth day. Th, which was lower efficacy in separate applications, caused 100 mortality on the sixth day when combined with DT 800 mgkg at the maximum dose 1  108 sporesmL. It has been determined that DT is effective in combination with entomopathogens, even at low concentrations. As a result, despite lowering application rates, these agents, which have promising potential against the insect, have shown acceptable results in binary combinations. The study shows a potential strategy that may assist lower DT application rates and encourage additional research into a method that may be applied practically against C. maculatus under long-term storage conditions.

 In this study, the efficiency of diatomaceous earth DT, Metarhizium anisopliae Ma, and Trichoderma hamatum Th isolates against Callasobruchus maculatus Coleoptera: Bruchidae adults was investigated under laboratory conditions. While Ma and Th isolates were used at three different concentrations 1  104, 1  106 and 1  108 sporesmL, the highest doses of each fungus 1  108 sporesmL were used in combination with three different doses of DT 200, 400, and 800 mgkg. The trials were conducted at 25C, 70 relative humidity, and 16:8 h light-to-dark periods. Individual applications exhibited 87.5 and 52.5 mortality on the eighth day after application for the highest doses of Ma and Th 1  108 sporesmL, respectively, and 100 mortality on the seventh day after application for the highest dose of DT 800 mgkg. Also, it was observed that mortality rates increase depending on time and dose. In combination trials, the most effective combination was DT 800 mgkg  Ma 1  108 sporesmL with a 100 mortality rate at the end of the fifth day. Th, which was lower efficacy in separate applications, caused 100 mortality on the sixth day when combined with DT 800 mgkg at the maximum dose 1  108 sporesmL. It has been determined that DT is effective in combination with entomopathogens, even at low concentrations. As a result, despite lowering application rates, these agents, which have promising potential against the insect, have shown acceptable results in binary combinations. The study shows a potential strategy that may assist lower DT application rates and encourage additional research into a method that may be applied practically against C. maculatus under long-term storage conditions.

 In this study, the efficiency of diatomaceous earth DT, Metarhizium anisopliae Ma, and Trichoderma hamatum Th isolates against Callasobruchus maculatus Coleoptera: Bruchidae adults was investigated under laboratory conditions. While Ma and Th isolates were used at three different concentrations 1  104, 1  106 and 1  108 sporesmL, the highest doses of each fungus 1  108 sporesmL were used in combination with three different doses of DT 200, 400, and 800 mgkg. The trials were conducted at 25C, 70 relative humidity, and 16:8 h light-to-dark periods. Individual applications exhibited 87.5 and 52.5 mortality on the eighth day after application for the highest doses of Ma and Th 1  108 sporesmL, respectively, and 100 mortality on the seventh day after application for the highest dose of DT 800 mgkg. Also, it was observed that mortality rates increase depending on time and dose. In combination trials, the most effective combination was DT 800 mgkg  Ma 1  108 sporesmL with a 100 mortality rate at the end of the fifth day. Th, which was lower efficacy in separate applications, caused 100 mortality on the sixth day when combined with DT 800 mgkg at the maximum dose 1  108 sporesmL. It has been determined that DT is effective in combination with entomopathogens, even at low concentrations. As a result, despite lowering application rates, these agents, which have promising potential against the insect, have shown acceptable results in binary combinations. The study shows a potential strategy that may assist lower DT application rates and encourage additional research into a method that may be applied practically against C. maculatus under long-term storage conditions.

 Bu çalışmada, diatom toprağı DT ile Metarhizium anisopliae Ma ve Trichoderma hamatum Th izolatlarının bireysel olarak ve kombinasyonlar halinde Callasobruchus maculatus Coleoptera: Bruchidae erginlerine karşı laboratuvar koşullarında etkinlikleri araştırılmıştır. Ma ve Th izolatlarının bireysel olarak üç konsantrasyonu 1  104, 1  106 ve 1  108 spormL kullanılırken, kombinasyonlarda da DTnin üç farklı dozu 200, 400 ve 800 mgkg ile her bir fungusa ait en yüksek doz 1  108 spormL kullanılarak uygulamalar yapılmıştır. Denemeler 25C, 70 nispi nem ve 16: 8 aydınlık-karanlık periyodunda yürütülmüştür. Bireysel uygulamalarda Ma ve Thnin en yüksek dozu 1  108 spormL için uygulamadan sonra 8. günde sırasıyla 87.5 ve 52.5 oranında ölüm meydana gelirken, DTnin en yüksek dozunda 800 mgkg uygulamadan 7 gün sonra 100 ölüm ortaya çıkmıştır. Dahası zamanın ve dozun artışına bağlı olarak ölüm oranlarının arttığı gözlemlenmiştir. Kombinasyon denemelerinde ise en yüksek etkinlik 5. günün sonunda 100 ölüm oranı ile DT 800 mgkg  Ma 1  108 spormL uygulamasında gözlemlenmesinin yanı sıra bireysel uygulamada düşük bir etkinlik gösteren Th en yüksek dozda 1  108 DT 800 mgkg ile kombinasyonu sonucunda 6. günde 100 ölüme sebep olmuştur. DTnin entomopatojenlerle çalışmadaki düşük dozlarda bile önemli bir etkinlik gösterdiği belirlenmiştir. Sonuç olarak, azalan uygulama oranlarına rağmen zararlıya karşı umut verici potansiyele sahip bu etmenler, ikili kombinasyonlarda kabul edilebilir sonuçlar ortaya koymuştur. Bu çalışma DTnin uygulama oranlarının azaltılmasına yardımcı olabilecek bu umut verici bir yaklaşımı ortaya koymakla birlikte uzun süreli depolama koşullarında C. maculatusa karşı pratik olarak kullanılabilecek bir yöntem için daha fazla araştırma yapılmasını teşvik edebilir.

Makale detayları

Determination of the Effectiveness of Metarhizium anisopliae, Trichoderma hamatum, and Diatomoceaus Earth, Applied Alone and in Combination against Callosobruchus maculatus F. (Coleoptera: Bruchidae)

Yazarlar:

Mehmet Yıldırım | Celal Tuncer | İsmail Oğuz Özdemir

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Tarımda Entomoloji

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.39 Issue 1, pp.195-206

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001312183

pISSN:

1308-8750

Stored product insect

Cowpea weevil

Entomopathogen fungi

Natural insecticide

Bioassay

Mortality rateDepo zararlısı

Börülce tohum böceği

Entomopatojen funguslar

Doğal insektisit

Biyolojik etkinlik

Ölüm oranı

Sayı

Makale detayları

Yazarlar: Mehmet Yıldırım | Celal Tuncer | İsmail Oğuz Özdemir

Yayıncı: Ondokuz Mayıs University

Ana Başlıklar: Tarımda Entomoloji

Dergi: Anadolu Tarım Bilimleri Dergisi Vol.39 Issue 1, pp.195-206

Belge Tipi: Makale

Yayın Tarihi: 2/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001312183

pISSN: 1308-8750

Sayı

Yazarlar:

Mehmet Yıldırım | Celal Tuncer | İsmail Oğuz Özdemir

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Tarımda Entomoloji

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.39 Issue 1, pp.195-206

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001312183

pISSN:

1308-8750