Investigation of Cytotoxic Effects of Tragopogon reticulatus Against HepG2 and MCF-7 Cell Lines and Antioxidant, Antimicrobial Effects Against Some Microorganisms

Lütfiye Kadıoğlu Dalkılıç; Semih Dalkılıç

Makale detayları

Investigation of Cytotoxic Effects of Tragopogon reticulatus Against HepG2 and MCF-7 Cell Lines and Antioxidant, Antimicrobial Effects Against Some Microorganisms

Yazarlar:

Lütfiye Kadıoğlu Dalkılıç | Semih Dalkılıç

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Yapısal Biyoloji | Hayvan Fizyolojisi - Hücre | Mikrobiyoloji (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 4, pp.1513-1525

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325402

pISSN:

2687-3729

Anahtar kelimeler:

Özet:

 Plants have been used for various purposes throughout history. This subject has received increasing attention in the field of traditional medicine in recent years. Tragopogon reticulatus, as a forage grass plant in turkey, is a species that stands out with rich vitamin and mineral content. The aim of this study was to investigate the antimicrobial, antioxidant, and cytotoxic activities of T. reticulatus. Two separate extracts were prepared at different concentrations using hexane and methanol solvents obtained from the T. reticulatus plant, and cytotoxic activity was evaluated by the MTT Assay 3-4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide method on human liver cancer HepG2 and human breast cancer MCF7 cell lines. The antioxidant activity of the extracts obtained from methanol and hexane was determined by the DPPH 2,2-diphenyl 1-picrylhydrazyl radical scavenging capacity method. Antimicrobial activity was evaluated by the disk diffusion method, and bacteria such as Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Bacillus megaterium, and Candida albicans fungus were used in the study. The hexane extract obtained from the T. reticulatus plant showed a high cytotoxic activity of 44 in the MCF7 cell line, while it showed a 51 dead cell count in the HepG2 cell line. When the antioxidant results were evaluated, it was determined that the DPPH radical scavenging effect of the hexane extract exhibited the most effective activity at 19. When the antimicrobial results were evaluated, it was determined that the concentrations prepared with hexane exhibited the most effective activity with a zone diameter of 16 mm against B. megaterium. These findings suggest that T. reticulatus plant extracts can be considered as a potential source. These results may shed light on future research into the biological effects of the plant and contribute to the development of new treatment methods.

 Plants have been used for various purposes throughout history. This subject has received increasing attention in the field of traditional medicine in recent years. Tragopogon reticulatus, as a forage grass plant in turkey, is a species that stands out with rich vitamin and mineral content. The aim of this study was to investigate the antimicrobial, antioxidant, and cytotoxic activities of T. reticulatus. Two separate extracts were prepared at different concentrations using hexane and methanol solvents obtained from the T. reticulatus plant, and cytotoxic activity was evaluated by the MTT Assay 3-4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide method on human liver cancer HepG2 and human breast cancer MCF7 cell lines. The antioxidant activity of the extracts obtained from methanol and hexane was determined by the DPPH 2,2-diphenyl 1-picrylhydrazyl radical scavenging capacity method. Antimicrobial activity was evaluated by the disk diffusion method, and bacteria such as Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Bacillus megaterium, and Candida albicans fungus were used in the study. The hexane extract obtained from the T. reticulatus plant showed a high cytotoxic activity of 44 in the MCF7 cell line, while it showed a 51 dead cell count in the HepG2 cell line. When the antioxidant results were evaluated, it was determined that the DPPH radical scavenging effect of the hexane extract exhibited the most effective activity at 19. When the antimicrobial results were evaluated, it was determined that the concentrations prepared with hexane exhibited the most effective activity with a zone diameter of 16 mm against B. megaterium. These findings suggest that T. reticulatus plant extracts can be considered as a potential source. These results may shed light on future research into the biological effects of the plant and contribute to the development of new treatment methods.

 Plants have been used for various purposes throughout history. This subject has received increasing attention in the field of traditional medicine in recent years. Tragopogon reticulatus, as a forage grass plant in turkey, is a species that stands out with rich vitamin and mineral content. The aim of this study was to investigate the antimicrobial, antioxidant, and cytotoxic activities of T. reticulatus. Two separate extracts were prepared at different concentrations using hexane and methanol solvents obtained from the T. reticulatus plant, and cytotoxic activity was evaluated by the MTT Assay 3-4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide method on human liver cancer HepG2 and human breast cancer MCF7 cell lines. The antioxidant activity of the extracts obtained from methanol and hexane was determined by the DPPH 2,2-diphenyl 1-picrylhydrazyl radical scavenging capacity method. Antimicrobial activity was evaluated by the disk diffusion method, and bacteria such as Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Bacillus megaterium, and Candida albicans fungus were used in the study. The hexane extract obtained from the T. reticulatus plant showed a high cytotoxic activity of 44 in the MCF7 cell line, while it showed a 51 dead cell count in the HepG2 cell line. When the antioxidant results were evaluated, it was determined that the DPPH radical scavenging effect of the hexane extract exhibited the most effective activity at 19. When the antimicrobial results were evaluated, it was determined that the concentrations prepared with hexane exhibited the most effective activity with a zone diameter of 16 mm against B. megaterium. These findings suggest that T. reticulatus plant extracts can be considered as a potential source. These results may shed light on future research into the biological effects of the plant and contribute to the development of new treatment methods.

 Bitkiler tarihsel süreç içerisinde çeşitli amaçlarla kullanılmıştır. Bu konu son yıllarda geleneksel tıp alanında giderek artan bir ilgi görmektedir. Tragopogon reticulatus, Türkiyede yemlik ot bitkisi olarak bilinen, zengin vitamin ve mineral içeriği ile dikkat çeken bir türdür. Bu çalışmanın amacı, T. reticulatus bitkisinin antimikrobiyal, antioksidan ve sitotoksik aktivitelerini araştırmaktır. T. reticulatus bitkisinden elde edilen hekzan ve metanol çözücülerinin kullanılmasıyla farklı konsantrasyonlarda iki ayrı ekstrakt hazırlanmış, sitotoksik aktivite ise insan karaciğer kanseri HepG2 ve insan meme kanseri MCF7 hücre hatları üzerinde MTT Assay 3-4,5-dimetiltiazol-2-il-2,5-difenil tetrazolyum bromür yöntemiyle değerlendirilmiştir. Metanol ve hekzandan elde edilen ekstraktların antioksidan aktivitesi DPPH 2,2-difenil 1-pikrilhidrazil radikal süpürme kapasitesi yöntemi ile belirlenmiştir. Antimikrobiyal aktivite kuyu difüzyon yöntemi ile değerlendirilmiş ve çalışmada Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli ve Bacillus megaterium gibi bakteriler ve Candida albicans mantarı kullanılmıştır. T. reticulatus bitkisinden elde edilen hekzan ekstresi, MCF7 hücre hattında 44 oranında yüksek sitotoksik aktivite gösterirken, HepG2 hücre hattında 51 oranında ölü hücre sayısı göstermiştir. Antioksidan sonuçlar değerlendirildiğinde, hekzan ekstresinin DPPH radikal süpürücü etkisinin 19 ile en etkili aktiviteyi sergilediği belirlenmiştir. Antimikrobiyal sonuçlar değerlendirildiğinde hekzan ile hazırlanan konsantrasyonların B. megateriuma karşı 16 mm zon çapı ile en etkili aktiviteyi sergilediği belirlenmiştir. Bu bulgular, T. reticulatus bitki ekstraktlarının potansiyel birer kaynak olarak kabul edilebileceğini göstermektedir. Bu sonuçlar, bitkinin biyolojik etkilerine dair gelecekteki araştırmalara ışık tutabilir ve yeni tedavi yöntemlerinin geliştirilmesine katkıda bulunabilir.

Makale detayları

Investigation of Cytotoxic Effects of Tragopogon reticulatus Against HepG2 and MCF-7 Cell Lines and Antioxidant, Antimicrobial Effects Against Some Microorganisms

Yazarlar:

Lütfiye Kadıoğlu Dalkılıç | Semih Dalkılıç

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Yapısal Biyoloji | Hayvan Fizyolojisi - Hücre | Mikrobiyoloji (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 4, pp.1513-1525

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325402

pISSN:

2687-3729

Tragopogan reticulatus

Cytotoxic

Antimicrobial

antioxidant activityTragopogan reticulatus

sitotoksik

antimikrobiyal

antioksidan aktivite

Sayı

Makale detayları

Yazarlar: Lütfiye Kadıoğlu Dalkılıç | Semih Dalkılıç

Yayıncı: Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar: Yapısal Biyoloji | Hayvan Fizyolojisi - Hücre | Mikrobiyoloji (Diğer)

Dergi: Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 4, pp.1513-1525

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001325402

pISSN: 2687-3729

Sayı

Yazarlar:

Lütfiye Kadıoğlu Dalkılıç | Semih Dalkılıç

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Yapısal Biyoloji | Hayvan Fizyolojisi - Hücre | Mikrobiyoloji (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 4, pp.1513-1525

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325402

pISSN:

2687-3729