INVESTIGATION CORRELATION BETWEEN THE ANTIOXIDANT, ANTIMICROBIAL, ANTI-FUNGI ACTIVITIES AND CONTENT OF PHENOLIC COMPOUNDS OF RASPBERRY LEAF (RUBUS IDAEUS L.) EXTRACTS

Olexander Maslov

Makale detayları

INVESTIGATION CORRELATION BETWEEN THE ANTIOXIDANT, ANTIMICROBIAL, ANTI-FUNGI ACTIVITIES AND CONTENT OF PHENOLIC COMPOUNDS OF RASPBERRY LEAF (RUBUS IDAEUS L.) EXTRACTS

Yazarlar:

Olexander Maslov

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1142-1154

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319076

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: The purpose of work was study the total content of some biologically active substances BAS, determine antimicrobial, anti-fungi and antioxidant activities of obtained raspberry leaf extracts, and study a correlation analysis between the content of natural compounds and antimicrobialantifungal and antioxidant activities. Material and Method: The quantity of phenolic compounds, catechins, flavonoids and hydroxycinnamic acids was determined by spectrophotometric method of analysis, whereas organic acids by alkalimetric method; antioxidant activity of obtained extracts was evaluated by potentiometric method, antimicrobial and anti-fungi wad determined by method of well. Result and Discussion: Results demonstrates the highest amount of polyphenols, flavonoids, catechins and hydroxycinnamic acids were 1.850.02, 1.010.02, 0.330.01 and 0.240.005 in 60 ethanolic extract, respectively. The organic acids were dominated in aqueous extract 1.020.02. The most potent antioxidant property possessed 60 extract of raspberry leaf. There is a high correlation between the content of polyphenols, catechins and antioxidant activity, in the case of inhibition of S. aureus, P. aeruginosa, B. subtillis depends on polyphenols, catechins and antioxidant activity, whereas fungi C. albicans significantly depends only on the content of hydroxycinnamic acids as well as E. coli is not depend on any BAS. These findings show the great potential in the development and creation of new medicines with antimicrobial, antioxidant and antifungal effects that are not inferior to, and even superior to, the effects of synthetic analogues.

 Objective: The purpose of work was study the total content of some biologically active substances BAS, determine antimicrobial, anti-fungi and antioxidant activities of obtained raspberry leaf extracts, and study a correlation analysis between the content of natural compounds and antimicrobialantifungal and antioxidant activities. Material and Method: The quantity of phenolic compounds, catechins, flavonoids and hydroxycinnamic acids was determined by spectrophotometric method of analysis, whereas organic acids by alkalimetric method; antioxidant activity of obtained extracts was evaluated by potentiometric method, antimicrobial and anti-fungi wad determined by method of well. Result and Discussion: Results demonstrates the highest amount of polyphenols, flavonoids, catechins and hydroxycinnamic acids were 1.850.02, 1.010.02, 0.330.01 and 0.240.005 in 60 ethanolic extract, respectively. The organic acids were dominated in aqueous extract 1.020.02. The most potent antioxidant property possessed 60 extract of raspberry leaf. There is a high correlation between the content of polyphenols, catechins and antioxidant activity, in the case of inhibition of S. aureus, P. aeruginosa, B. subtillis depends on polyphenols, catechins and antioxidant activity, whereas fungi C. albicans significantly depends only on the content of hydroxycinnamic acids as well as E. coli is not depend on any BAS. These findings show the great potential in the development and creation of new medicines with antimicrobial, antioxidant and antifungal effects that are not inferior to, and even superior to, the effects of synthetic analogues.

 Objective: The purpose of work was study the total content of some biologically active substances BAS, determine antimicrobial, anti-fungi and antioxidant activities of obtained raspberry leaf extracts, and study a correlation analysis between the content of natural compounds and antimicrobialantifungal and antioxidant activities. Material and Method: The quantity of phenolic compounds, catechins, flavonoids and hydroxycinnamic acids was determined by spectrophotometric method of analysis, whereas organic acids by alkalimetric method; antioxidant activity of obtained extracts was evaluated by potentiometric method, antimicrobial and anti-fungi wad determined by method of well. Result and Discussion: Results demonstrates the highest amount of polyphenols, flavonoids, catechins and hydroxycinnamic acids were 1.850.02, 1.010.02, 0.330.01 and 0.240.005 in 60 ethanolic extract, respectively. The organic acids were dominated in aqueous extract 1.020.02. The most potent antioxidant property possessed 60 extract of raspberry leaf. There is a high correlation between the content of polyphenols, catechins and antioxidant activity, in the case of inhibition of S. aureus, P. aeruginosa, B. subtillis depends on polyphenols, catechins and antioxidant activity, whereas fungi C. albicans significantly depends only on the content of hydroxycinnamic acids as well as E. coli is not depend on any BAS. These findings show the great potential in the development and creation of new medicines with antimicrobial, antioxidant and antifungal effects that are not inferior to, and even superior to, the effects of synthetic analogues.

 Amaç: Çalışmanın amacı, bazı biyolojik olarak aktif maddelerin BAS toplam içeriğini incelemek, elde edilen ahududu yaprağı ekstrelerinin antimikrobiyal, anti-fungal ve antioksidan aktivitelerini belirlemek ve doğal bileşiklerin içeriği ile antimikrobiyalantifungal ve antioksidan aktivitleri arasındaki korelasyon analizini incelemektir. Gereç ve Yöntem: Fenolik bileşikler, kateşinler, flavonoitler ve hidroksisinamik asitlerin miktarı spektrofotometrik analiz yöntemiyle, organik asitlerin miktarı ise alkalimetrik yöntemle belirlenmiştir. Elde edilen ekstrelerin antioksidan aktiviteleri potansiyometrik yöntemle değerlendirilmiş, antimikrobiyal ve anti-fungal aktivite ise kuyu yöntemiyle belirlenmiştir. Sonuç ve Tartışma: Sonuçlar; en yüksek polifenol, flavonoit, kateşin ve hidroksisinnamik asit miktarının sırasıyla 1.850.02, 1.010.02, 0.330.01 ve 0.240.005 olarak 60 etanolik ekstrede olduğunu göstermiştir. Sulu ekstrede organik asitler baskın bulunmuştur 1.020.02. En güçlü antioksidan özelliği 60lık ahududu yaprağı ekstresi göstermiştir. Polifenol ve kateşin içeriği ile antioksidan etki arasında yüksek korelasyon tespit edilmiştir; S. aureus, P. aeruginosa, B. subtillisin inhibisyonunun polifenoller ile kateşinlerin içeriğine ve ve antioksidan aktiviteye bağlı olduğu görülürken; C. albicans mantarlarına karşı etkinin yalnızca hidroksisinnamik asit içeriği ile önemli ölçüde ilişkili olduğu tespit edilmiştir. E. coli üzerine etkinin ise herhangi bir BASa bağlı olmadığı gözlenmiştir. Bu bulgular; sentetik analogların etkilerinden daha düşük olmayan ve hatta onlardan daha üstün etkinlik göstermesi dolayısıyla, antimikrobiyal, antioksidan ve anti-fungal etkilere sahip yeni ilaçların geliştirilmesi ve yaratılmasındaki büyük potansiyeli göstermektedir.

Makale detayları

INVESTIGATION CORRELATION BETWEEN THE ANTIOXIDANT, ANTIMICROBIAL, ANTI-FUNGI ACTIVITIES AND CONTENT OF PHENOLIC COMPOUNDS OF RASPBERRY LEAF (RUBUS IDAEUS L.) EXTRACTS

Yazarlar:

Olexander Maslov

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1142-1154

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319076

pISSN:

1015-3918

Antimicrobial activity

anti-fungi

antioxidant activity

correlation analysis

polyphenols

raspberry leafAhududu yaprağı

antibakteriyel aktivite

anti-fungal

antioksidan aktivite

korelasyon analizi

polifenoller

Sayı

Makale detayları

Yazarlar: Olexander Maslov

Yayıncı: Ankara University

Ana Başlıklar: Farmasotik Mikrobiyoloji

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1142-1154

Belge Tipi: Makale

Yayın Tarihi: 10/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001319076

pISSN: 1015-3918

Sayı

Yazarlar:

Olexander Maslov

Yayıncı:

Ankara University

Ana Başlıklar:

Farmasotik Mikrobiyoloji

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1142-1154

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319076

pISSN:

1015-3918