MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN

Hülya Cabadak; Beki Kan

Makale detayları

MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN

Yazarlar:

Hülya Cabadak | Beki Kan

Yayıncı:

Marmara University

Dergi:

Marmara Medical Journal 1999 Vol.12 Issue 1, pp.25-28

Belge Tipi:

Makale

Yayın Tarihi:

12/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000546774

Sıklık:

Aylık

eISSN:

1309-9469

pISSN:

1019-1941

Özet:

 


Objective: Activation of cholinergic muscarinic receptors leads to activation of membrane - bound phospholipase C, leading to generation of IP3 and diacylglycérol, The present study aimed at measuring muscarinic receptor-mediated phosphoinositide breakdown in three different regions of rat brain.Methods: Cortical, hippocampal and striatal slices labeled with [3H] myo-inositol were incubated with muscarinic agonist carbachol (Cch) and LiCI. Reaction was terminated by the addition of chloroform/methanol and labeled inositol phosphates were extracted from tissue. Individual [3H]- inositol phosphates were separated using anion exchange chromatography.Results: Cch caused a rise in IP, formation, where the increase over basal in the cortex, hippocampus and striatum were 98%, 73% and 45%, respectively.Conclusion: Phosphoinositide turnover wasstimulated by carbachol in all brain structures studied, with the most pronounced effects observed in the cortex and hippocampus.Key Words: Muscarinic receptors, inositolphosphates, carbachol, rat brain

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Makale detayları

MUSCARINIC RECEPTOR - MEDIATED PHOSPHOINOSITIDE HYDROLYSIS IN RAT BRAIN

Yazarlar: Hülya Cabadak | Beki Kan

Yayıncı: Marmara University

Dergi: Marmara Medical Journal 1999 Vol.12 Issue 1, pp.25-28

Belge Tipi: Makale

Yayın Tarihi: 12/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000546774

Sıklık: Aylık

eISSN: 1309-9469

pISSN: 1019-1941

Sayı

Yazarlar:

Hülya Cabadak | Beki Kan

Yayıncı:

Marmara University

Dergi:

Marmara Medical Journal 1999 Vol.12 Issue 1, pp.25-28

Belge Tipi:

Makale

Yayın Tarihi:

12/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000546774

Sıklık:

Aylık

eISSN:

1309-9469

pISSN:

1019-1941