An entomopathogenic bacterium, Pseudomonas putida, from Leptinotarsa decemlineata

Hacer MURATOĞLU; Zihni DEMİRBAĞ; Kazım SEZEN

Makale detayları

An entomopathogenic bacterium, Pseudomonas putida, from Leptinotarsa decemlineata

Yazarlar:

Hacer MURATOĞLU | Zihni DEMİRBAĞ | Kazım SEZEN

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2011 Vol.35 Issue 3, pp.275-282

Belge Tipi:

Makale

Yayın Tarihi:

2/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000812860

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The Colorado Potato Beetle (CPB), Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), is a pre-eminent pest on solanaceous crops worldwide, and much research has focused on the different components of its variable population dynamics. L. decemlineata has developed resistance to insecticides used for its control. Consequently, alternative methods are needed urgently for the control of this pest. In this study, in order to find a more effective and safer biological control agent against L. decemlineata, we isolated a new bacterial isolate (Ld4) from L. decemlineata, and tested it for insecticidal effects on L. decemlineata larvae. Also, the insecticidal effect of Ld4 was compared with Ld3, which was isolated from L. decemlineata. According to the morphological, physiological, biochemical tests, API20E and API50CH panel test systems, and 16S rRNA sequence homologies, this isolate was identified as Pseudomonas putida. The highest insecticidal effect determined on L. decemlineata within 5 days was 100%, and this activity was exhibited by Ld4 isolate. Our results indicate that Ld4 isolate may be valuable as a biological control agent for the biological control of L. decemlineata.

 

The Colorado Potato Beetle (CPB), Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), is a pre-eminent pest on solanaceous crops worldwide, and much research has focused on the different components of its variable population dynamics. L. decemlineata has developed resistance to insecticides used for its control. Consequently, alternative methods are needed urgently for the control of this pest. In this study, in order to find a more effective and safer biological control agent against L. decemlineata, we isolated a new bacterial isolate (Ld4) from L. decemlineata, and tested it for insecticidal effects on L. decemlineata larvae. Also, the insecticidal effect of Ld4 was compared with Ld3, which was isolated from L. decemlineata. According to the morphological, physiological, biochemical tests, API20E and API50CH panel test systems, and 16S rRNA sequence homologies, this isolate was identified as Pseudomonas putida. The highest insecticidal effect determined on L. decemlineata within 5 days was 100%, and this activity was exhibited by Ld4 isolate. Our results indicate that Ld4 isolate may be valuable as a biological control agent for the biological control of L. decemlineata.

Makale detayları

An entomopathogenic bacterium, Pseudomonas putida, from Leptinotarsa decemlineata

Yazarlar:

Hacer MURATOĞLU | Zihni DEMİRBAĞ | Kazım SEZEN

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2011 Vol.35 Issue 3, pp.275-282

Belge Tipi:

Makale

Yayın Tarihi:

2/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000812860

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Pseudomonas putida

insecticidal activity

microbial control

Sayı

Makale detayları

An entomopathogenic bacterium, Pseudomonas putida, from Leptinotarsa decemlineata

Yazarlar: Hacer MURATOĞLU | Zihni DEMİRBAĞ | Kazım SEZEN

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2011 Vol.35 Issue 3, pp.275-282

Belge Tipi: Makale

Yayın Tarihi: 2/2011

Belge Dili: İngilizce

Document ID (Org No.): 2000812860

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Pseudomonas putida

insecticidal activity

microbial control

Sayı

Yazarlar:

Hacer MURATOĞLU | Zihni DEMİRBAĞ | Kazım SEZEN

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2011 Vol.35 Issue 3, pp.275-282

Belge Tipi:

Makale

Yayın Tarihi:

2/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000812860

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152