Flavonoids from Cirsium italicum (Savi) DC. reduce reactive oxygen species and modulate histone deacetylase activity in triple-negative breast cancer cells

Bahadır Batar; Mehmet Kazancı; Ahmet Ensar Karaoğlu; Alperen Genç; Furkan Doğan; Zeynel Utku Reyhan; Dilara Ceren Çaba; Yasemin Günaydın; Muhammed Baran; Eslem Mortaş; Ayşe Nur Çakır; Ferhat Eyüp Yeşilmen; Ada Aslan; Öykü Canbulat; Büşra Berdibek; Elif Serdal; Merve Argon; Hilmican Çalışkan; Temine Şabudak; Birol Topçu

Makale detayları

Flavonoids from Cirsium italicum (Savi) DC. reduce reactive oxygen species and modulate histone deacetylase activity in triple-negative breast cancer cells

Yazarlar:

Bahadır Batar | Mehmet Kazancı | Ahmet Ensar Karaoğlu | Alperen Genç | Furkan Doğan | Zeynel Utku Reyhan | Dilara Ceren Çaba | Yasemin Günaydın | Muhammed Baran | Eslem Mortaş | Ayşe Nur Çakır | Ferhat Eyüp Yeşilmen | Ada Aslan | Öykü Canbulat | Büşra Berdibek | Elif Serdal | Merve Argon | Hilmican Çalışkan | Temine Şabudak | Birol Topçu

Yayıncı:

Cukurova University

Ana Başlıklar:

Tümör İmmünolojisi | Kanser Hücre Biyolojisi

Dergi:

Cukurova Medical Journal Vol.50 Issue 3, pp.752-760

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357844

pISSN:

2602-3032

Anahtar kelimeler:

Özet:

 Purpose: The present study aims to investigate the potential anti-cancer activities of flavonoid compounds from Cirsium italicum Savi DC. plants in breast cancer cells. Materials and Methods: MDA-MB-231 triple-negative breast cancer TNBC cells were used to demonstrate reactive oxygen species ROS production effects and histone deacetylase HDAC inhibitory activity of flavonoids 5,3,4-trihydroxy-7-methoxy isoflavone Santal 1, 5,5,7-trihydroxy-3,7-dimethoxy-4,4-O-biflavone 2, 6-2ʹ-dihydroxy-5-methoxy isoflavonone-4ʹ-O-α-D-glucopyranoside 3, 8,3,4,5-tetrahydroxy-7-O-α-glycosyl isoflavone-6-8-4-hydroxy-7-O-α-glycosyl isoflavone biflavone 4 isolated from C. italicum. Results: Significant differences in intracellular ROS levels were observed in MDA-MB-231 breast cancer cells treated with the compounds at concentrations of 0.06, 0.08, and 0.1 mgml. Compound 2 at 0.06 mgml, and compounds 2 and 4 at 0.08 and 0.1 mgml, induced a significant decrease in intracellular ROS levels compared to control cells. At a concentration of 0.06 mgml, compound 2 showed a mean value of 0.0030.004. At 0.08 mgml, the mean values were 0.0060.018 for compound 2 and 0.0130.013 for compound 4. At 0.1 mgml, compound 2 and compound 4 exhibited mean values of 0.0140.017 and 0.0140.007, respectively. Even though all flavonoids inhibited HDAC activity, this inhibition was not statistically significant. Conclusion: Among the flavonoids isolated from C. italicum, compounds 2 and 4 showed potential anticancer effects in breast cancer cells, potentially through modulation of intracellular ROS levels.

 Purpose: The present study aims to investigate the potential anti-cancer activities of flavonoid compounds from Cirsium italicum Savi DC. plants in breast cancer cells. Materials and Methods: MDA-MB-231 triple-negative breast cancer TNBC cells were used to demonstrate reactive oxygen species ROS production effects and histone deacetylase HDAC inhibitory activity of flavonoids 5,3,4-trihydroxy-7-methoxy isoflavone Santal 1, 5,5,7-trihydroxy-3,7-dimethoxy-4,4-O-biflavone 2, 6-2ʹ-dihydroxy-5-methoxy isoflavonone-4ʹ-O-α-D-glucopyranoside 3, 8,3,4,5-tetrahydroxy-7-O-α-glycosyl isoflavone-6-8-4-hydroxy-7-O-α-glycosyl isoflavone biflavone 4 isolated from C. italicum. Results: Significant differences in intracellular ROS levels were observed in MDA-MB-231 breast cancer cells treated with the compounds at concentrations of 0.06, 0.08, and 0.1 mgml. Compound 2 at 0.06 mgml, and compounds 2 and 4 at 0.08 and 0.1 mgml, induced a significant decrease in intracellular ROS levels compared to control cells. At a concentration of 0.06 mgml, compound 2 showed a mean value of 0.0030.004. At 0.08 mgml, the mean values were 0.0060.018 for compound 2 and 0.0130.013 for compound 4. At 0.1 mgml, compound 2 and compound 4 exhibited mean values of 0.0140.017 and 0.0140.007, respectively. Even though all flavonoids inhibited HDAC activity, this inhibition was not statistically significant. Conclusion: Among the flavonoids isolated from C. italicum, compounds 2 and 4 showed potential anticancer effects in breast cancer cells, potentially through modulation of intracellular ROS levels.

 Purpose: The present study aims to investigate the potential anti-cancer activities of flavonoid compounds from Cirsium italicum Savi DC. plants in breast cancer cells. Materials and Methods: MDA-MB-231 triple-negative breast cancer TNBC cells were used to demonstrate reactive oxygen species ROS production effects and histone deacetylase HDAC inhibitory activity of flavonoids 5,3,4-trihydroxy-7-methoxy isoflavone Santal 1, 5,5,7-trihydroxy-3,7-dimethoxy-4,4-O-biflavone 2, 6-2ʹ-dihydroxy-5-methoxy isoflavonone-4ʹ-O-α-D-glucopyranoside 3, 8,3,4,5-tetrahydroxy-7-O-α-glycosyl isoflavone-6-8-4-hydroxy-7-O-α-glycosyl isoflavone biflavone 4 isolated from C. italicum. Results: Significant differences in intracellular ROS levels were observed in MDA-MB-231 breast cancer cells treated with the compounds at concentrations of 0.06, 0.08, and 0.1 mgml. Compound 2 at 0.06 mgml, and compounds 2 and 4 at 0.08 and 0.1 mgml, induced a significant decrease in intracellular ROS levels compared to control cells. At a concentration of 0.06 mgml, compound 2 showed a mean value of 0.0030.004. At 0.08 mgml, the mean values were 0.0060.018 for compound 2 and 0.0130.013 for compound 4. At 0.1 mgml, compound 2 and compound 4 exhibited mean values of 0.0140.017 and 0.0140.007, respectively. Even though all flavonoids inhibited HDAC activity, this inhibition was not statistically significant. Conclusion: Among the flavonoids isolated from C. italicum, compounds 2 and 4 showed potential anticancer effects in breast cancer cells, potentially through modulation of intracellular ROS levels.

 Amaç: Bu çalışma, Cirsium italicum Savi DC. bitkisinden elde edilen flavonoid bileşiklerin meme kanseri hücrelerindeki potansiyel anti-kanser aktivitelerini araştırmayı amaçlamaktadır. Gereç ve Yöntem: MDA-MB-231 üçlü negatif meme kanseri hücreleri, Cirsium italicumdan izole edilen flavonoidler 5,3,4-trihidroksi-7-metoksi izoflavon Santal 1, 5,5,7-trihidroksi-3,7-dimetoksi-4,4-O-biflavon 2, 6-2ʹ-dihidroksi-5-metoksi izoflavonon-4ʹ-O-α-D-glukopiranozid 3, 8,3,4,5-tetrahidroksi-7-O-α-glikozil izoflavon-6-8-4-hidroksi-7-O-α-glikozil izoflavon biflavon 4; reaktif oksijen türleri ROS üretim etkilerini ve histon deasetilaz HDAC inhibitör aktivitesini göstermek için kullanıldı. Bulgular: 0.06, 0.08 ve 0.1 konsantrasyonlarında uygulanan bileşiklerle muamele edilen MDA-MB-231 meme kanseri hücrelerinde, hücre içi ROS düzeylerinde anlamlı farklılıklar gözlemlendi. 0.06 mgml konsantrasyonunda uygulanan bileşik 2 ile, 0.08 ve 0.1 mgml konsantrasyonlarında uygulanan bileşik 2 ve 4ün, kontrol hücrelerine kıyasla hücre içi ROS düzeylerinde anlamlı bir azalmaya yol açtığı gözlemlendi. 0.06 mgml konsantrasyonunda, bileşik 2nin ortalama değeri 0.0030.004 olarak ölçüldü. 0.08 mgml konsantrasyonunda, ortalama değerler bileşik 2 için 0.0060.018 ve bileşik 4 için 0.0130.013 olarak belirlendi. 0.1 mgml konsantrasyonunda ise, bileşik 2 ve bileşik 4 sırasıyla 0.0140.017 ve 0.0140.007 değerlerini gösterdi. Her ne kadar tüm flavonoidler HDAC aktivitesini inhibe etmiş olsa da, bu inhibisyon istatistiksel olarak anlamlı bulunmadı. Sonuç: C. italicum bitkisinden izole edilen flavonoidler arasında, bileşik 2 ve 4 hücre içi ROS düzeylerini modüle etme yoluyla meme kanseri hücrelerinde potansiyel antikanser etkiler gösterdi.

Makale detayları

Flavonoids from Cirsium italicum (Savi) DC. reduce reactive oxygen species and modulate histone deacetylase activity in triple-negative breast cancer cells

Yayıncı:

Cukurova University

Ana Başlıklar:

Tümör İmmünolojisi | Kanser Hücre Biyolojisi

Dergi:

Cukurova Medical Journal Vol.50 Issue 3, pp.752-760

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357844

pISSN:

2602-3032

Asteraceae

breast cancer

Cirsium italicum

flavonoid

histone deacetylase

reactive oxygen speciesAsteraceae

meme kanseri

Cirsium italicum

flavonoid

histon deasetilaz

reaktif oksijen türleri

Sayı

Makale detayları

Yayıncı: Cukurova University

Ana Başlıklar: Tümör İmmünolojisi | Kanser Hücre Biyolojisi

Dergi: Cukurova Medical Journal Vol.50 Issue 3, pp.752-760

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001357844

pISSN: 2602-3032

Sayı

Yayıncı:

Cukurova University

Ana Başlıklar:

Tümör İmmünolojisi | Kanser Hücre Biyolojisi

Dergi:

Cukurova Medical Journal Vol.50 Issue 3, pp.752-760

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357844

pISSN:

2602-3032