Boron and Cytokinin Combinations Modulate Shoot Tip Necrosis and Improve Subculture Efficiency in Grapevine Micropropagation

Kevser Yazar

Makale detayları

Boron and Cytokinin Combinations Modulate Shoot Tip Necrosis and Improve Subculture Efficiency in Grapevine Micropropagation

Yazarlar:

Kevser Yazar

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Oenology and Viticulture | Plant Biotechnology in Agriculture

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.9 Issue 3, pp.743-751

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001354269

pISSN:

2618-5946

Anahtar kelimeler:

Özet:

 This study was conducted to determine the effects of different combinations of BAP 6-Benzylaminopurine and boric acid H₃BO₃ on the morphological development during the initial and first subculture stages of the Ekşi Kara grape cultivar under in vitro conditions. The evaluated parameters included shoot formation rate, number of shoots per explant, shoot length, fresh shoot weight, leaf area, number of nodes, and shoot tip necrosis. The findings indicated that BAP applications had notable effects particularly on shoot formation, shoot length, and fresh shoot weight. The highest fresh shoot weight 2.934 g was obtained from the combination of 1.0 mgL BAP and 1.18 mgL boric acid, which also led to the formation of multiple shoots 6 shoots per explant. PCA analysis revealed a strong association between this treatment and the parameters representing shoot development. While boric acid alone had limited influence, its effectiveness increased when combined with appropriate BAP doses. The results suggest that specific combinations of plant growth regulators and micronutrients may enhance morphological development during the in vitro propagation process.

 This study was conducted to determine the effects of different combinations of BAP 6-Benzylaminopurine and boric acid H₃BO₃ on the morphological development during the initial and first subculture stages of the Ekşi Kara grape cultivar under in vitro conditions. The evaluated parameters included shoot formation rate, number of shoots per explant, shoot length, fresh shoot weight, leaf area, number of nodes, and shoot tip necrosis. The findings indicated that BAP applications had notable effects particularly on shoot formation, shoot length, and fresh shoot weight. The highest fresh shoot weight 2.934 g was obtained from the combination of 1.0 mgL BAP and 1.18 mgL boric acid, which also led to the formation of multiple shoots 6 shoots per explant. PCA analysis revealed a strong association between this treatment and the parameters representing shoot development. While boric acid alone had limited influence, its effectiveness increased when combined with appropriate BAP doses. The results suggest that specific combinations of plant growth regulators and micronutrients may enhance morphological development during the in vitro propagation process.

 This study was conducted to determine the effects of different combinations of BAP 6-Benzylaminopurine and boric acid H₃BO₃ on the morphological development during the initial and first subculture stages of the Ekşi Kara grape cultivar under in vitro conditions. The evaluated parameters included shoot formation rate, number of shoots per explant, shoot length, fresh shoot weight, leaf area, number of nodes, and shoot tip necrosis. The findings indicated that BAP applications had notable effects particularly on shoot formation, shoot length, and fresh shoot weight. The highest fresh shoot weight 2.934 g was obtained from the combination of 1.0 mgL BAP and 1.18 mgL boric acid, which also led to the formation of multiple shoots 6 shoots per explant. PCA analysis revealed a strong association between this treatment and the parameters representing shoot development. While boric acid alone had limited influence, its effectiveness increased when combined with appropriate BAP doses. The results suggest that specific combinations of plant growth regulators and micronutrients may enhance morphological development during the in vitro propagation process.

 This study was conducted to determine the effects of different combinations of BAP 6-Benzylaminopurine and boric acid H₃BO₃ on the morphological development during the initial and first subculture stages of the Ekşi Kara grape cultivar under in vitro conditions. The evaluated parameters included shoot formation rate, number of shoots per explant, shoot length, fresh shoot weight, leaf area, number of nodes, and shoot tip necrosis. The findings indicated that BAP applications had notable effects particularly on shoot formation, shoot length, and fresh shoot weight. The highest fresh shoot weight 2.934 g was obtained from the combination of 1.0 mgL BAP and 1.18 mgL boric acid, which also led to the formation of multiple shoots 6 shoots per explant. PCA analysis revealed a strong association between this treatment and the parameters representing shoot development. While boric acid alone had limited influence, its effectiveness increased when combined with appropriate BAP doses. The results suggest that specific combinations of plant growth regulators and micronutrients may enhance morphological development during the in vitro propagation process.

Makale detayları

Boron and Cytokinin Combinations Modulate Shoot Tip Necrosis and Improve Subculture Efficiency in Grapevine Micropropagation

Yazarlar:

Kevser Yazar

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Oenology and Viticulture | Plant Biotechnology in Agriculture

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.9 Issue 3, pp.743-751

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001354269

pISSN:

2618-5946

In vitro culture

BAP

Boric acid

Micropropagation

Shoot development

Morphological parameters

Sayı

Makale detayları

Boron and Cytokinin Combinations Modulate Shoot Tip Necrosis and Improve Subculture Efficiency in Grapevine Micropropagation

Yazarlar: Kevser Yazar

Yayıncı: Gültekin ÖZDEMİR

Ana Başlıklar: Oenology and Viticulture | Plant Biotechnology in Agriculture

Dergi: International Journal of Agriculture Environment and Food Sciences Vol.9 Issue 3, pp.743-751

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001354269

pISSN: 2618-5946

In vitro culture

BAP

Boric acid

Micropropagation

Shoot development

Morphological parameters

Sayı

Yazarlar:

Kevser Yazar

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Oenology and Viticulture | Plant Biotechnology in Agriculture

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.9 Issue 3, pp.743-751

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001354269

pISSN:

2618-5946