Nanofiber encapsulation of probiotic cultures via electrospinning: fabrication and quality compliance with ISO/IEC 17043 and ISO 22117 standards

Ahmet Koluman; Çiğdem Akduman; Mahmed Sari Njjar; Meltem Delimanlar; Ulviye Adamcı; Mehmet Kıvanç Alay; Mustafa Soylu

Makale detayları

Nanofiber encapsulation of probiotic cultures via electrospinning: fabrication and quality compliance with ISO/IEC 17043 and ISO 22117 standards

Yazarlar:

Ahmet Koluman | Çiğdem Akduman | Mahmed Sari Njjar | Meltem Delimanlar | Ulviye Adamcı | Mehmet Kıvanç Alay | Mustafa Soylu

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Microbiology

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 3, pp.323-333

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353477

pISSN:

1300-0861

Anahtar kelimeler:

Özet:

 Probiotics offer numerous health benefits, including inhibiting pathogenic growth, supporting intestinal microbiota, and synthesizing essential biomolecules. However, their viability during storage remains a challenge due to sensitivity to environmental conditions. This study investigates the encapsulation of Lactobacillus rhamnosus and Lactobacillus acidophilus in polyvinyl alcohol PVA nanofibers via electrospinning to enhance stability and viability. Near-optimized electrospinning parameters, including solution concentration, voltage, and collector distance, were used to produce nanofibers, which were characterized using Field Emission Scanning Electron Microscopy FESEM. The results showed non-uniform fiber diameter distributions, with 16 kV producing thicker fibers with an average diameter of 479.11 nm. Homogeneity assessment confirmed uniform probiotic distribution within the nanofibers, with a coefficient of variation of 5.3. Storage stability tests at 4C over 15 days were conducted following ISOIEC 17043 and ISO 22117 standards. The findings demonstrated that encapsulation effectively preserved L. rhamnosus viability in 16LRPVA nanofibers, whereas L. acidophilus exhibited reduced viability at both 10 kV and 16 kV.

 Probiotics offer numerous health benefits, including inhibiting pathogenic growth, supporting intestinal microbiota, and synthesizing essential biomolecules. However, their viability during storage remains a challenge due to sensitivity to environmental conditions. This study investigates the encapsulation of Lactobacillus rhamnosus and Lactobacillus acidophilus in polyvinyl alcohol PVA nanofibers via electrospinning to enhance stability and viability. Near-optimized electrospinning parameters, including solution concentration, voltage, and collector distance, were used to produce nanofibers, which were characterized using Field Emission Scanning Electron Microscopy FESEM. The results showed non-uniform fiber diameter distributions, with 16 kV producing thicker fibers with an average diameter of 479.11 nm. Homogeneity assessment confirmed uniform probiotic distribution within the nanofibers, with a coefficient of variation of 5.3. Storage stability tests at 4C over 15 days were conducted following ISOIEC 17043 and ISO 22117 standards. The findings demonstrated that encapsulation effectively preserved L. rhamnosus viability in 16LRPVA nanofibers, whereas L. acidophilus exhibited reduced viability at both 10 kV and 16 kV.

 Probiotics offer numerous health benefits, including inhibiting pathogenic growth, supporting intestinal microbiota, and synthesizing essential biomolecules. However, their viability during storage remains a challenge due to sensitivity to environmental conditions. This study investigates the encapsulation of Lactobacillus rhamnosus and Lactobacillus acidophilus in polyvinyl alcohol PVA nanofibers via electrospinning to enhance stability and viability. Near-optimized electrospinning parameters, including solution concentration, voltage, and collector distance, were used to produce nanofibers, which were characterized using Field Emission Scanning Electron Microscopy FESEM. The results showed non-uniform fiber diameter distributions, with 16 kV producing thicker fibers with an average diameter of 479.11 nm. Homogeneity assessment confirmed uniform probiotic distribution within the nanofibers, with a coefficient of variation of 5.3. Storage stability tests at 4C over 15 days were conducted following ISOIEC 17043 and ISO 22117 standards. The findings demonstrated that encapsulation effectively preserved L. rhamnosus viability in 16LRPVA nanofibers, whereas L. acidophilus exhibited reduced viability at both 10 kV and 16 kV.

 Probiotics offer numerous health benefits, including inhibiting pathogenic growth, supporting intestinal microbiota, and synthesizing essential biomolecules. However, their viability during storage remains a challenge due to sensitivity to environmental conditions. This study investigates the encapsulation of Lactobacillus rhamnosus and Lactobacillus acidophilus in polyvinyl alcohol PVA nanofibers via electrospinning to enhance stability and viability. Near-optimized electrospinning parameters, including solution concentration, voltage, and collector distance, were used to produce nanofibers, which were characterized using Field Emission Scanning Electron Microscopy FESEM. The results showed non-uniform fiber diameter distributions, with 16 kV producing thicker fibers with an average diameter of 479.11 nm. Homogeneity assessment confirmed uniform probiotic distribution within the nanofibers, with a coefficient of variation of 5.3. Storage stability tests at 4C over 15 days were conducted following ISOIEC 17043 and ISO 22117 standards. The findings demonstrated that encapsulation effectively preserved L. rhamnosus viability in 16LRPVA nanofibers, whereas L. acidophilus exhibited reduced viability at both 10 kV and 16 kV.

Makale detayları

Nanofiber encapsulation of probiotic cultures via electrospinning: fabrication and quality compliance with ISO/IEC 17043 and ISO 22117 standards

Yazarlar:

Ahmet Koluman | Çiğdem Akduman | Mahmed Sari Njjar | Meltem Delimanlar | Ulviye Adamcı | Mehmet Kıvanç Alay | Mustafa Soylu

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Microbiology

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 3, pp.323-333

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353477

pISSN:

1300-0861

Electrospinning

Encapsulation

Nanofibers

Probiotic Microorganisms

Sayı

Makale detayları

Nanofiber encapsulation of probiotic cultures via electrospinning: fabrication and quality compliance with ISO/IEC 17043 and ISO 22117 standards

Yazarlar: Ahmet Koluman | Çiğdem Akduman | Mahmed Sari Njjar | Meltem Delimanlar | Ulviye Adamcı | Mehmet Kıvanç Alay | Mustafa Soylu

Yayıncı: Ankara University

Ana Başlıklar: Veterinary Microbiology

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 3, pp.323-333

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001353477

pISSN: 1300-0861

Electrospinning

Encapsulation

Nanofibers

Probiotic Microorganisms

Sayı

Yazarlar:

Ahmet Koluman | Çiğdem Akduman | Mahmed Sari Njjar | Meltem Delimanlar | Ulviye Adamcı | Mehmet Kıvanç Alay | Mustafa Soylu

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Microbiology

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 3, pp.323-333

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001353477

pISSN:

1300-0861