Identification of Chicken Originated Campylobacter coli and Campylobacter jejuni by Polymerase Chain Reaction (PCR)

Hasan Basri ERTAŞ; Burhan ÇETİNKAYA; Adile MUZ; Hasan ÖNGÖR

Makale detayları

Identification of Chicken Originated Campylobacter coli and Campylobacter jejuni by Polymerase Chain Reaction (PCR)

Yazarlar:

Hasan Basri ERTAŞ | Burhan ÇETİNKAYA | Adile MUZ | Hasan ÖNGÖR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 6, pp.1447-1452

Belge Tipi:

Makale

Yayın Tarihi:

5/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000770465

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The purpose of this study was to isolate Campylobacter species from the intestines and livers of chicken and to identify Campylobacter coli and Campylobacter jejuni by both conventional methods and Polymerase Chain Reaction (PCR). Four specific primers derived from the ceuE gene present in the genomes of C. coli and C. jejuni were used for PCR identification.    In the examination of 150 intestine and liver samples by culture and PCR, 25 (16.6%) and 32 (21.3%) were identified as C. coli and C. jejuni, respectively.    It was concluded that the PCR assay used in this study may successfully be applied for the differentiation of these species as an alternative to conventional methods, owing to its accuracy and speed.

 

The purpose of this study was to isolate Campylobacter species from the intestines and livers of chicken and to identify Campylobacter coli and Campylobacter jejuni by both conventional methods and Polymerase Chain Reaction (PCR). Four specific primers derived from the ceuE gene present in the genomes of C. coli and C. jejuni were used for PCR identification.    In the examination of 150 intestine and liver samples by culture and PCR, 25 (16.6%) and 32 (21.3%) were identified as C. coli and C. jejuni, respectively.    It was concluded that the PCR assay used in this study may successfully be applied for the differentiation of these species as an alternative to conventional methods, owing to its accuracy and speed.

Makale detayları

Identification of Chicken Originated Campylobacter coli and Campylobacter jejuni by Polymerase Chain Reaction (PCR)

Yazarlar: Hasan Basri ERTAŞ | Burhan ÇETİNKAYA | Adile MUZ | Hasan ÖNGÖR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 6, pp.1447-1452

Belge Tipi: Makale

Yayın Tarihi: 5/2002

Belge Dili: Türkçe

Document ID (Org No.): 2000770465

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Campylobacter coli

Campylobacter jejuni

PCR

chicken

ceuE

Sayı

Yazarlar:

Hasan Basri ERTAŞ | Burhan ÇETİNKAYA | Adile MUZ | Hasan ÖNGÖR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 6, pp.1447-1452

Belge Tipi:

Makale

Yayın Tarihi:

5/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000770465

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128