DIENTAMOEBA FRAGILIS AND GASTROINTESTINAL SYMPTOMS: EFFICACY OF DIAGNOSTIC METHODS AND GENOTYPE-SYMPTOM CORRELATION

Filiz Ayrangöl; Özden Boral; Halim İşsever; Asli Cifcibasi Ormeci; Metin Uysalol; Selda Hançerli Törün; Zerrin Önal

Makale detayları

DIENTAMOEBA FRAGILIS AND GASTROINTESTINAL SYMPTOMS: EFFICACY OF DIAGNOSTIC METHODS AND GENOTYPE-SYMPTOM CORRELATION

Yazarlar:

Filiz Ayrangöl | Özden Boral | Halim İşsever | Asli Cifcibasi Ormeci | Metin Uysalol | Selda Hançerli Törün | Zerrin Önal

Yayıncı:

Istanbul University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

İstanbul Tıp Fakültesi Dergisi Vol.88 Issue 3, pp.198-206

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356761

pISSN:

1305-6441

Anahtar kelimeler:

Özet:

 Objective: This study aimed to investigate the association between Dientamoeba fragilis and gastrointestinal symptoms, assess the effectiveness of various diagnostic methods, and determine the correlation between genotypes and clinical manifestations. Material and Methods: Stool samples were collected from 116 patients with gastrointestinal complaints. The presence of D. frag ilis was investigated using culture methods BD and LS media and molecular techniques real-time PCR and conventional PCR. Positive samples were subjected to sequencing analysis for geno typing. Results: D. fragilis was detected in 18 out of 116 patients 15.52 using PCR, whereas culture methods identified only 9 positive cases 7.75. A statistically significant difference was observed between the molecular and culture methods p0.001. All D. frag ilis samples belonged to Genotype 1. The most common symptoms were diarrhoea 76.9 and abdominal pain 61.5; however, no significant correlation was found between genotype and clinical symptoms. Conclusion: Real-time PCR was the most sensitive and specific diagnostic method. Genotyping results indicated no association between D. fragilis genotypes and clinical manifestations. Further studies are needed to explore additional genomic targets to better understand the pathogenic potential of D. fragilis.

 Objective: This study aimed to investigate the association between Dientamoeba fragilis and gastrointestinal symptoms, assess the effectiveness of various diagnostic methods, and determine the correlation between genotypes and clinical manifestations. Material and Methods: Stool samples were collected from 116 patients with gastrointestinal complaints. The presence of D. frag ilis was investigated using culture methods BD and LS media and molecular techniques real-time PCR and conventional PCR. Positive samples were subjected to sequencing analysis for geno typing. Results: D. fragilis was detected in 18 out of 116 patients 15.52 using PCR, whereas culture methods identified only 9 positive cases 7.75. A statistically significant difference was observed between the molecular and culture methods p0.001. All D. frag ilis samples belonged to Genotype 1. The most common symptoms were diarrhoea 76.9 and abdominal pain 61.5; however, no significant correlation was found between genotype and clinical symptoms. Conclusion: Real-time PCR was the most sensitive and specific diagnostic method. Genotyping results indicated no association between D. fragilis genotypes and clinical manifestations. Further studies are needed to explore additional genomic targets to better understand the pathogenic potential of D. fragilis.

 Objective: This study aimed to investigate the association between Dientamoeba fragilis and gastrointestinal symptoms, assess the effectiveness of various diagnostic methods, and determine the correlation between genotypes and clinical manifestations. Material and Methods: Stool samples were collected from 116 patients with gastrointestinal complaints. The presence of D. frag ilis was investigated using culture methods BD and LS media and molecular techniques real-time PCR and conventional PCR. Positive samples were subjected to sequencing analysis for geno typing. Results: D. fragilis was detected in 18 out of 116 patients 15.52 using PCR, whereas culture methods identified only 9 positive cases 7.75. A statistically significant difference was observed between the molecular and culture methods p0.001. All D. frag ilis samples belonged to Genotype 1. The most common symptoms were diarrhoea 76.9 and abdominal pain 61.5; however, no significant correlation was found between genotype and clinical symptoms. Conclusion: Real-time PCR was the most sensitive and specific diagnostic method. Genotyping results indicated no association between D. fragilis genotypes and clinical manifestations. Further studies are needed to explore additional genomic targets to better understand the pathogenic potential of D. fragilis.

 Amaç: Bu çalışma, Dientamoeba fragilis ile gastrointestinal semp tomlar arasındaki ilişkiyi araştırmayı, farklı tanı yöntemlerinin etkinliğini değerlendirmeyi ve genotipler ile klinik bulgular arasın daki korelasyonu belirlemeyi amaçlamaktadır. Gereç ve Yöntemler: Gastrointestinal şikayetleri olan 116 hastadan dışkı örnekleri toplandı. D. fragilis varlığı kültür yöntemleri BD ve LS besiyerleri ve moleküler teknikler real-time PCR ve konvan siyonel PCR kullanılarak araştırıldı. Pozitif örnekler genotipleme amacıyla sekans analizine tabi tutuldu. Bulgular: PCR ile 116 hastanın 18inde 15,52 D. fragilis saptandı. Buna karşın, kültür yöntemleri yalnızca 9 pozitif vaka 7,75 belirledi. Moleküler yöntemler ile kültür yöntemleri arasında ista tistiksel olarak anlamlı bir fark bulundu p0,001. Tüm D. fragilis örnekleri Genotip 1 olarak tanımlandı. En yaygın semptomlar ishal 76,9 ve karın ağrısı 61,5 idi; ancak, genotip ile klinik semp tomlar arasında anlamlı bir ilişki saptanmadı. Sonuç: Real-time PCR, en duyarlı ve spesifik tanı yöntemi olarak belirlendi. Genotipleme sonuçları, D. fragilis genotipleri ile klinik semptomlar arasında bir ilişki bulunmadığını göstermiştir. D. fragilisin patojenite potansiyelini daha iyi anlamak için ek genomik hedeflerin araştırılmasına yönelik ileri çalışmalara ihtiyaç vardır.

Makale detayları

DIENTAMOEBA FRAGILIS AND GASTROINTESTINAL SYMPTOMS: EFFICACY OF DIAGNOSTIC METHODS AND GENOTYPE-SYMPTOM CORRELATION

Yazarlar:

Filiz Ayrangöl | Özden Boral | Halim İşsever | Asli Cifcibasi Ormeci | Metin Uysalol | Selda Hançerli Törün | Zerrin Önal

Yayıncı:

Istanbul University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

İstanbul Tıp Fakültesi Dergisi Vol.88 Issue 3, pp.198-206

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356761

pISSN:

1305-6441

Dientamoeba fragilis

culture

genotypingDientamoeba fragilis

kültür

genotiplendirme

Sayı

Makale detayları

DIENTAMOEBA FRAGILIS AND GASTROINTESTINAL SYMPTOMS: EFFICACY OF DIAGNOSTIC METHODS AND GENOTYPE-SYMPTOM CORRELATION

Yazarlar: Filiz Ayrangöl | Özden Boral | Halim İşsever | Asli Cifcibasi Ormeci | Metin Uysalol | Selda Hançerli Törün | Zerrin Önal

Yayıncı: Istanbul University

Ana Başlıklar: Klinik Mikrobiyoloji

Dergi: İstanbul Tıp Fakültesi Dergisi Vol.88 Issue 3, pp.198-206

Belge Tipi: Makale

Yayın Tarihi: 7/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001356761

pISSN: 1305-6441

Dientamoeba fragilis

culture

genotypingDientamoeba fragilis

kültür

genotiplendirme

Sayı

Yazarlar:

Filiz Ayrangöl | Özden Boral | Halim İşsever | Asli Cifcibasi Ormeci | Metin Uysalol | Selda Hançerli Törün | Zerrin Önal

Yayıncı:

Istanbul University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

İstanbul Tıp Fakültesi Dergisi Vol.88 Issue 3, pp.198-206

Belge Tipi:

Makale

Yayın Tarihi:

7/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356761

pISSN:

1305-6441