Effects of Gundelia dersim Exosome-Like Nanoparticles on Cell Viability in Human Colon and Lung Cancer Cell Lines

Fazilet Erman; Gül Kaya; Semra Türkoğlu

Makale detayları

Effects of Gundelia dersim Exosome-Like Nanoparticles on Cell Viability in Human Colon and Lung Cancer Cell Lines

Yazarlar:

Fazilet Erman | Gül Kaya | Semra Türkoğlu

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 2, pp.1408-1420

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293034

pISSN:

2687-3729

Anahtar kelimeler:

Özet:

 Exosomes are nano sized 30-100 nm bioactive vesicles that constitute the smallest subgroup of extracellular vesicles EVs. They have properties such as transporting genetic materials, maintaining intercellular communication, regulating immunity, and transmitting signals. Although plant-derived nanoparticles, which exhibit a wide range of therapeutic potential, have not been studied as extensively as in mammalian cells, there is an increasing number of research supporting their cross-kingdom effects. This study aims to investigate the cytotoxic effects of exosome-like nanoparticles isolated from the Gundelia dersim plant GdENs, which is endemic to Turkey, on cell viability in human lung cancer A549 and human colon cancer HCT 116 cell lines. The isolated GdENs were imaged through nanoparticle tracking analysis NTA. Protein analysis was performed with BCA Bicinchoninic acid. The nanoparticles were applied on A549 and HCT116 cell lines. By conducting a Water-soluble tetrazolium salt-1 WST-1 assay, the cytotoxic effect on these cell lines was studied. According to our findings, the increasing concentrations of isolated GdENs on, demonstrated cytotoxic effects by reducing cell viability in HCT 116 cell lines at 75, 100 and 250 μgmL within 24, 48 and 72 hours. In A549 cell lines, it was determined that nanoparticles exhibited cytotoxic effects at 24 hours in all concentration units, but did not exhibit any cytotoxic effect by increasing cell viability at 72 hours. In conclusion, GdENs are thought to have therapeutic potential as an anticarcinogen if supported by more comprehensive studies.

 Exosomes are nano sized 30-100 nm bioactive vesicles that constitute the smallest subgroup of extracellular vesicles EVs. They have properties such as transporting genetic materials, maintaining intercellular communication, regulating immunity, and transmitting signals. Although plant-derived nanoparticles, which exhibit a wide range of therapeutic potential, have not been studied as extensively as in mammalian cells, there is an increasing number of research supporting their cross-kingdom effects. This study aims to investigate the cytotoxic effects of exosome-like nanoparticles isolated from the Gundelia dersim plant GdENs, which is endemic to Turkey, on cell viability in human lung cancer A549 and human colon cancer HCT 116 cell lines. The isolated GdENs were imaged through nanoparticle tracking analysis NTA. Protein analysis was performed with BCA Bicinchoninic acid. The nanoparticles were applied on A549 and HCT116 cell lines. By conducting a Water-soluble tetrazolium salt-1 WST-1 assay, the cytotoxic effect on these cell lines was studied. According to our findings, the increasing concentrations of isolated GdENs on, demonstrated cytotoxic effects by reducing cell viability in HCT 116 cell lines at 75, 100 and 250 μgmL within 24, 48 and 72 hours. In A549 cell lines, it was determined that nanoparticles exhibited cytotoxic effects at 24 hours in all concentration units, but did not exhibit any cytotoxic effect by increasing cell viability at 72 hours. In conclusion, GdENs are thought to have therapeutic potential as an anticarcinogen if supported by more comprehensive studies.

 Exosomes are nano sized 30-100 nm bioactive vesicles that constitute the smallest subgroup of extracellular vesicles EVs. They have properties such as transporting genetic materials, maintaining intercellular communication, regulating immunity, and transmitting signals. Although plant-derived nanoparticles, which exhibit a wide range of therapeutic potential, have not been studied as extensively as in mammalian cells, there is an increasing number of research supporting their cross-kingdom effects. This study aims to investigate the cytotoxic effects of exosome-like nanoparticles isolated from the Gundelia dersim plant GdENs, which is endemic to Turkey, on cell viability in human lung cancer A549 and human colon cancer HCT 116 cell lines. The isolated GdENs were imaged through nanoparticle tracking analysis NTA. Protein analysis was performed with BCA Bicinchoninic acid. The nanoparticles were applied on A549 and HCT116 cell lines. By conducting a Water-soluble tetrazolium salt-1 WST-1 assay, the cytotoxic effect on these cell lines was studied. According to our findings, the increasing concentrations of isolated GdENs on, demonstrated cytotoxic effects by reducing cell viability in HCT 116 cell lines at 75, 100 and 250 μgmL within 24, 48 and 72 hours. In A549 cell lines, it was determined that nanoparticles exhibited cytotoxic effects at 24 hours in all concentration units, but did not exhibit any cytotoxic effect by increasing cell viability at 72 hours. In conclusion, GdENs are thought to have therapeutic potential as an anticarcinogen if supported by more comprehensive studies.

 Eksozomlar; kalıtsal metaryal taşıma, hücreler arası iletişimi sağlama, bağışıklığı düzenleme ve sinyal iletme gibi özelliklere sahip olan ekstrasellüler vezikülllerin EV en küçük alt grubunu oluşturan, nano boyutlardaki 30-100 nm biyoaktif keseciklerdir. Geniş bir terapötik potansiyel sergileyen bitki türevli nanopartiküller, memeli hücrelerindeki kadar çok çalışılmamış olsa da bunların cross-kingdom etkilerini destekleyen araştırmaların sayısı giderek artmaktadır. Bu çalışmanın amacı; Türkiye için endemik olan Gundelia dersim bitkisinden izole edilen eksozom benzeri nanopartiküllerin GdEN insan akciğer kanseri A549 ve insan kolon kanseri HCT 116 hücre hatlarındaki hücre canlılığı üzerine sitotoksik etkilerini araştırmaktır. İzole edilen GdENler, nanopartikül izleme analiziyle NTA görüntülenmiştir. BCA Bikinkoninik asit ile protein analizi yapılmıştır. Nanopartiküller A549 ve HCT116 hücre hatlarına uygulanmıştır. Suda çözünür tetrazolyum tuzu1 WST-1 analizi yapılıp bu hücre hatlarında GdENlerin sitotoksik etkisine bakılmıştır. Bulgularımıza göre, GdENlerin artan konsantrasyonları HCT 116 hücre hatlarında 24, 48 ve 72 saatte 75, 100 ve 250 μgmLde hücre canlılığını azaltarak sitotoksik bir etki göstermiştir. A549 hücre hatlarında ise nanopartiküllerin, tüm konsantrasyon birimlerinde 24 saatte sitotoksik etki gösterdiği, 72 saatte ise hücre canlılığını arttırarak sitotoksik etki göstermediği tespit edilmiştir. Sonuç olarak, GdENlerin daha ileri çalışmalarla desteklenmesi durumunda anti kanserojen etki gösterebileceği düşünülmektedir.

Makale detayları

Effects of Gundelia dersim Exosome-Like Nanoparticles on Cell Viability in Human Colon and Lung Cancer Cell Lines

Yazarlar:

Fazilet Erman | Gül Kaya | Semra Türkoğlu

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 2, pp.1408-1420

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293034

pISSN:

2687-3729

Gundelia dersim

Exosome

Cancer

Cytotoxic effectsGundelia dersim

Eksozom

Kanser

Sitotoksik etki

Sayı

Makale detayları

Yazarlar: Fazilet Erman | Gül Kaya | Semra Türkoğlu

Yayıncı: Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar: Yapısal Biyoloji

Dergi: Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 2, pp.1408-1420

Belge Tipi: Makale

Yayın Tarihi: 5/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293034

pISSN: 2687-3729

Sayı

Yazarlar:

Fazilet Erman | Gül Kaya | Semra Türkoğlu

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.6 Issue 2, pp.1408-1420

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293034

pISSN:

2687-3729