Bacterial toxin colicin N-T domain structure changes to ordered state upon binding C-terminal domain of TolA

Yakup ULUSU; Sema Bilgin ŞENTÜRK; Fatma GEDİKLİ; Jeremy H. LAKEY; İsa GÖKÇE

Makale detayları

Bacterial toxin colicin N-T domain structure changes to ordered state upon binding C-terminal domain of TolA

Yazarlar:

Yakup ULUSU | Sema Bilgin ŞENTÜRK | Fatma GEDİKLİ | Jeremy H. LAKEY | İsa GÖKÇE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 5, pp.648-652

Belge Tipi:

Makale

Yayın Tarihi:

4/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812134

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Colicin N is a bacterial toxin that kills Escherichia coli and related cells. Its mode of action is of interest in protein import and toxicology. Colicin N translocates across the E. coli outer membrane and periplasm by interacting with several receptors. The translocation process involves the interaction of the colicin N with the outer membrane porin OmpF and subsequently with the integral membrane protein TolA. The N-terminal domain of colicin N is involved in the import process. TolA consists of 3 domains. The N-terminal domain of colicin N interacts with the C-terminal domain of TolA at later stages of the translocation process. Our aim was to produce a large quantity of colicin N T-domains for spectroscopic and crystallization studies. These both require a correctly folded and stable protein. Here we present an expression of the complex between the N-terminal domain of colicin N and the C-terminal domain of TolA obtained by fusing these 2 domains with a flexible linker. Circular dichroism spectroscopy studies indicated that unstructured bacterial toxin colicin N T-domains changed to an ordered state upon binding to the C-terminal domain of TolA; this fusion protein has a secondary and tertiary structure.

 

Colicin N is a bacterial toxin that kills Escherichia coli and related cells. Its mode of action is of interest in protein import and toxicology. Colicin N translocates across the E. coli outer membrane and periplasm by interacting with several receptors. The translocation process involves the interaction of the colicin N with the outer membrane porin OmpF and subsequently with the integral membrane protein TolA. The N-terminal domain of colicin N is involved in the import process. TolA consists of 3 domains. The N-terminal domain of colicin N interacts with the C-terminal domain of TolA at later stages of the translocation process. Our aim was to produce a large quantity of colicin N T-domains for spectroscopic and crystallization studies. These both require a correctly folded and stable protein. Here we present an expression of the complex between the N-terminal domain of colicin N and the C-terminal domain of TolA obtained by fusing these 2 domains with a flexible linker. Circular dichroism spectroscopy studies indicated that unstructured bacterial toxin colicin N T-domains changed to an ordered state upon binding to the C-terminal domain of TolA; this fusion protein has a secondary and tertiary structure.

Makale detayları

Bacterial toxin colicin N-T domain structure changes to ordered state upon binding C-terminal domain of TolA

Yazarlar:

Yakup ULUSU | Sema Bilgin ŞENTÜRK | Fatma GEDİKLİ | Jeremy H. LAKEY | İsa GÖKÇE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 5, pp.648-652

Belge Tipi:

Makale

Yayın Tarihi:

4/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812134

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Colicins

TolA

interaction

E. coli

Sayı

Makale detayları

Bacterial toxin colicin N-T domain structure changes to ordered state upon binding C-terminal domain of TolA

Yazarlar: Yakup ULUSU | Sema Bilgin ŞENTÜRK | Fatma GEDİKLİ | Jeremy H. LAKEY | İsa GÖKÇE

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2014 Vol.38 Issue 5, pp.648-652

Belge Tipi: Makale

Yayın Tarihi: 4/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000812134

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Colicins

TolA

interaction

E. coli

Sayı

Yazarlar:

Yakup ULUSU | Sema Bilgin ŞENTÜRK | Fatma GEDİKLİ | Jeremy H. LAKEY | İsa GÖKÇE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 5, pp.648-652

Belge Tipi:

Makale

Yayın Tarihi:

4/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000812134

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152