SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION

Emine İrdem; Semra İlhan; Ercan Özbiçen; Lira Usakbek Kyzy; Gamze Tunca; Esma Ocak; Niyazi Can Zorluer; Uğur Çiğdem; Fatma Ayva; Rasime Demirel

Makale detayları

SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION

Yazarlar:

Emine İrdem | Semra İlhan | Ercan Özbiçen | Lira Usakbek Kyzy | Gamze Tunca | Esma Ocak | Niyazi Can Zorluer | Uğur Çiğdem | Fatma Ayva | Rasime Demirel

Yayıncı:

Trakya University

Ana Başlıklar:

Microbiology

Dergi:

Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.51-62

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287851

pISSN:

2528-9691

Anahtar kelimeler:

Özet:

 Haloalkalitolerant fungi can survive in environments with high salt concentrations and pH values. The bioactive compounds produced under stressful conditions have potential biotechnological applications. In this study, 52 microfungi isolated from Acıgöl Lake in Türkiye, offering polyextreme conditions, were screened for some biotechnological properties. The antimicrobial and antioxidant activities of the isolates were determined using the agar diffusion and the 2,2-diphenyl-1-picrylhydrazyl DPPH radical scavenging methods, respectively. Enzyme activities were determined by various methods using the agar diffusion technique. Synthesis of silver nanoparticles AgNPs was carried out using cell-free filtrate of microfungi. 40 of the isolates showed antimicrobial activity against at least one of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213 and Candida albicans ATCC 90028 used as the test microorganisms. Penicillium dipodomyicola showed the highest antibacterial activity against E. coli and S. aureus, while P. brevicompactum showed the highest activity against C. albicans. Penicillium dipodomyicola and P. bilaiae were found to have free radical scavenging activity of a level 90 and above that can compete with positive control. All of the isolates with amylase activity belonged to Aspergillus and Penicillium and the most prominent three of them were A. ochraceous, A. flavus and P. brevicompactum. 55 of the isolates showed proteolytic activity, among which A. alliaceus had the highest activity. Almost all isolates 92 showed lipolytic activity. Aspergillus amstelodami, P. sizovae and P. solitum had a significant level of lipolytic activity. 35 of the isolates showed cellulolytic activity with highest values Cladosporium pseudocladosporioides, P. dipodomyicola and P. bilaiae. Eight of the isolates carried out AgNP synthesis within 24 h. When all the results were evaluated, Aspergillus amstelodami, A. ochraceus, Penicillium dipodomyicola, and P. brevicompactum appeared to have the potential to serve in different industrial areas.

 Haloalkalitolerant fungi can survive in environments with high salt concentrations and pH values. The bioactive compounds produced under stressful conditions have potential biotechnological applications. In this study, 52 microfungi isolated from Acıgöl Lake in Türkiye, offering polyextreme conditions, were screened for some biotechnological properties. The antimicrobial and antioxidant activities of the isolates were determined using the agar diffusion and the 2,2-diphenyl-1-picrylhydrazyl DPPH radical scavenging methods, respectively. Enzyme activities were determined by various methods using the agar diffusion technique. Synthesis of silver nanoparticles AgNPs was carried out using cell-free filtrate of microfungi. 40 of the isolates showed antimicrobial activity against at least one of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213 and Candida albicans ATCC 90028 used as the test microorganisms. Penicillium dipodomyicola showed the highest antibacterial activity against E. coli and S. aureus, while P. brevicompactum showed the highest activity against C. albicans. Penicillium dipodomyicola and P. bilaiae were found to have free radical scavenging activity of a level 90 and above that can compete with positive control. All of the isolates with amylase activity belonged to Aspergillus and Penicillium and the most prominent three of them were A. ochraceous, A. flavus and P. brevicompactum. 55 of the isolates showed proteolytic activity, among which A. alliaceus had the highest activity. Almost all isolates 92 showed lipolytic activity. Aspergillus amstelodami, P. sizovae and P. solitum had a significant level of lipolytic activity. 35 of the isolates showed cellulolytic activity with highest values Cladosporium pseudocladosporioides, P. dipodomyicola and P. bilaiae. Eight of the isolates carried out AgNP synthesis within 24 h. When all the results were evaluated, Aspergillus amstelodami, A. ochraceus, Penicillium dipodomyicola, and P. brevicompactum appeared to have the potential to serve in different industrial areas.

 Haloalkalitolerant fungi can survive in environments with high salt concentrations and pH values. The bioactive compounds produced under stressful conditions have potential biotechnological applications. In this study, 52 microfungi isolated from Acıgöl Lake in Türkiye, offering polyextreme conditions, were screened for some biotechnological properties. The antimicrobial and antioxidant activities of the isolates were determined using the agar diffusion and the 2,2-diphenyl-1-picrylhydrazyl DPPH radical scavenging methods, respectively. Enzyme activities were determined by various methods using the agar diffusion technique. Synthesis of silver nanoparticles AgNPs was carried out using cell-free filtrate of microfungi. 40 of the isolates showed antimicrobial activity against at least one of Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213 and Candida albicans ATCC 90028 used as the test microorganisms. Penicillium dipodomyicola showed the highest antibacterial activity against E. coli and S. aureus, while P. brevicompactum showed the highest activity against C. albicans. Penicillium dipodomyicola and P. bilaiae were found to have free radical scavenging activity of a level 90 and above that can compete with positive control. All of the isolates with amylase activity belonged to Aspergillus and Penicillium and the most prominent three of them were A. ochraceous, A. flavus and P. brevicompactum. 55 of the isolates showed proteolytic activity, among which A. alliaceus had the highest activity. Almost all isolates 92 showed lipolytic activity. Aspergillus amstelodami, P. sizovae and P. solitum had a significant level of lipolytic activity. 35 of the isolates showed cellulolytic activity with highest values Cladosporium pseudocladosporioides, P. dipodomyicola and P. bilaiae. Eight of the isolates carried out AgNP synthesis within 24 h. When all the results were evaluated, Aspergillus amstelodami, A. ochraceus, Penicillium dipodomyicola, and P. brevicompactum appeared to have the potential to serve in different industrial areas.

 Haloalkalitolerant funguslar, tuz konsantrasyonunun ve pH değerinin yüksek olduğu ortamlarda yaşayabilirler. Stresli koşullar altında üretilen biyoaktif bileşiklerin potansiyel biyoteknolojik uygulamaları vardır. Bu çalışmada, poliekstrem koşullara sahip AcıgölTürkiyeden izole edilen 52 mikrofungus, bazı biyoteknolojik özellikler açısından taranmıştır. Antimikrobiyal ve antioksidan aktiviteler sırasıyla agar difüzyon ve 2,2-difenil-1-pikrilhidrazil DPPH radikal süpürücü etki yöntemi kullanılarak belirlenmiştir. Enzim aktiviteleri, agar difüzyon tekniğinin kullanıldığı çeşitli yöntemlerle belirlenmiştir. Gümüş nanopartikül AgNP sentezi, hücre içermeyen mikrofungus filtratı kullanılarak gerçekleştirilmiştir. İzolatların 40ı test mikroorganizmalarından en az birine karşı antimikrobiyal aktivite göstermiştir. Penicillium dipodomyicola, E. coli ve S. aureusa karşı en yüksek antibakteriyel aktiviteyi gösterirken, P. brevicompactum Candida albicansa karşı en yüksek aktiviteyi göstermiştir. Penicillium dipodomyicola ve P. bilaiaenin pozitif kontrol ile rekabet edebilecek düzeyde 90 ve üzeri serbest radikal süpürücü aktiviteye sahip olduğu belirlenmiştir. Amilaz aktivitesine sahip izolatların tamamı Aspergillus ve Penicillium cinslerine ait olup, A. ochraceous, A. flavus ve P. brevicompactum en yüksek aktiviteye sahip türler olarak belirlenmiştir. İzolatların 55i proteolitik aktivite gösterirken, A. alliaceus en yüksek aktiviteye sahip izolat olmuştur. Hemen hemen tüm izolatlar 92 lipolitik aktivite göstermiştir. Özellikle A. amstelodami, P. sizovae ve P. solitum izolatlarının önemli düzeyde lipolitik aktiviteye sahip olduğu belirlenmiştir. İzolatların 35i selülolitik aktivite göstermiş ve Cladosporium pseudocladosporioides, P. dipodomyicola ve P. bilaiae izolatlarında yüksek aktivite gözlenmiştir. İzolatların sekizi 24 saat içinde AgNP sentezini gerçekleştirmiştir. Tüm sonuçlar değerlendirildiğinde Aspergillus amstelodami, A. ochraceus, Penicillium dipodomyicola ve P. brevicompactum gibi izolatların farklı endüstriyel alanlarda hizmet verme potansiyeline sahip olduğu belirlenmiştir.

Makale detayları

SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION

Yazarlar:

Emine İrdem | Semra İlhan | Ercan Özbiçen | Lira Usakbek Kyzy | Gamze Tunca | Esma Ocak | Niyazi Can Zorluer | Uğur Çiğdem | Fatma Ayva | Rasime Demirel

Yayıncı:

Trakya University

Ana Başlıklar:

Microbiology

Dergi:

Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.51-62

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287851

pISSN:

2528-9691

polyextremophiles

alkaline lake

biological activity

microfungi

Sayı

Makale detayları

SCREENING OF MICROFUNGI ISOLATED FROM ACIGÖL, TÜRKİYE FOR HYDROLYTIC ENZYMES, BIOACTIVE METABOLITES AND SILVER NANOPARTICLE PRODUCTION

Yazarlar: Emine İrdem | Semra İlhan | Ercan Özbiçen | Lira Usakbek Kyzy | Gamze Tunca | Esma Ocak | Niyazi Can Zorluer | Uğur Çiğdem | Fatma Ayva | Rasime Demirel

Yayıncı: Trakya University

Ana Başlıklar: Microbiology

Dergi: Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.51-62

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001287851

pISSN: 2528-9691

polyextremophiles

alkaline lake

biological activity

microfungi

Sayı

Yazarlar:

Emine İrdem | Semra İlhan | Ercan Özbiçen | Lira Usakbek Kyzy | Gamze Tunca | Esma Ocak | Niyazi Can Zorluer | Uğur Çiğdem | Fatma Ayva | Rasime Demirel

Yayıncı:

Trakya University

Ana Başlıklar:

Microbiology

Dergi:

Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.51-62

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287851

pISSN:

2528-9691