Analysis of Extended Spectrum Beta Lactamase Frequency in Klebsiella spp Isolates

Mehmet Tevfik Bayraktar; Esma Ceylan; Bashar Ibrahım

Makale detayları

Analysis of Extended Spectrum Beta Lactamase Frequency in Klebsiella spp Isolates

Yazarlar:

Mehmet Tevfik Bayraktar | Esma Ceylan | Bashar Ibrahım

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 1, pp.1-10

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287318

pISSN:

2146-247X

Anahtar kelimeler:

Özet:

 The issue of increasing resistance to antibiotics in recent years has become an important problem all over the world. Our aim is to determine the antimicrobial resistance profile and Extended Spectrum Beta-Lactamase ESBL rates in Klebsiella spp isolates to prevent the gradual increase in multi-resistant isolates as a result of unconscious antibiotic use thereby contributing to the faster effective treatment of infections. A total of 100 Klebsiella spp were isolated and identified from various clinical specimens. Antibiotic susceptibility tests were performed using the Kirby-Bauer method. The presence of extended-spectrum beta-lactamases ESBL was detected using the Double Disc Synergy Test DDST and E-test methods. The rates of ESBL-producing strains were 46.1 in 6 K. oxytoca and 56.3 in 49 K. pneumoniae. These strains were found to be 38 in 38 adult patients and 17 in 17 pediatric patients, and this difference was statistically significant p 0.05. The ESBL rate was 31 in 31 male patients and 24 in 24 female patients, and this difference was not statistically significant p0.05. This rate was found to be high in patients hospitalized in the pediatric service and intensive care unit. 67 out of 100 strains were found to be suspicious for ESBL by Disk Diffusion Test DDT. DDST and E-tests were applied as confirmatory tests. The sensitivity of the DDST and E tests was 100. Screening for ESBL in Klebsiella spp and other members of Enterobacteriaceae isolates is necessary to reduce further selection and spread of these increasingly broad-spectrum antimicrobial-resistant enteric pathogens.

 The issue of increasing resistance to antibiotics in recent years has become an important problem all over the world. Our aim is to determine the antimicrobial resistance profile and Extended Spectrum Beta-Lactamase ESBL rates in Klebsiella spp isolates to prevent the gradual increase in multi-resistant isolates as a result of unconscious antibiotic use thereby contributing to the faster effective treatment of infections. A total of 100 Klebsiella spp were isolated and identified from various clinical specimens. Antibiotic susceptibility tests were performed using the Kirby-Bauer method. The presence of extended-spectrum beta-lactamases ESBL was detected using the Double Disc Synergy Test DDST and E-test methods. The rates of ESBL-producing strains were 46.1 in 6 K. oxytoca and 56.3 in 49 K. pneumoniae. These strains were found to be 38 in 38 adult patients and 17 in 17 pediatric patients, and this difference was statistically significant p 0.05. The ESBL rate was 31 in 31 male patients and 24 in 24 female patients, and this difference was not statistically significant p0.05. This rate was found to be high in patients hospitalized in the pediatric service and intensive care unit. 67 out of 100 strains were found to be suspicious for ESBL by Disk Diffusion Test DDT. DDST and E-tests were applied as confirmatory tests. The sensitivity of the DDST and E tests was 100. Screening for ESBL in Klebsiella spp and other members of Enterobacteriaceae isolates is necessary to reduce further selection and spread of these increasingly broad-spectrum antimicrobial-resistant enteric pathogens.

 The issue of increasing resistance to antibiotics in recent years has become an important problem all over the world. Our aim is to determine the antimicrobial resistance profile and Extended Spectrum Beta-Lactamase ESBL rates in Klebsiella spp isolates to prevent the gradual increase in multi-resistant isolates as a result of unconscious antibiotic use thereby contributing to the faster effective treatment of infections. A total of 100 Klebsiella spp were isolated and identified from various clinical specimens. Antibiotic susceptibility tests were performed using the Kirby-Bauer method. The presence of extended-spectrum beta-lactamases ESBL was detected using the Double Disc Synergy Test DDST and E-test methods. The rates of ESBL-producing strains were 46.1 in 6 K. oxytoca and 56.3 in 49 K. pneumoniae. These strains were found to be 38 in 38 adult patients and 17 in 17 pediatric patients, and this difference was statistically significant p 0.05. The ESBL rate was 31 in 31 male patients and 24 in 24 female patients, and this difference was not statistically significant p0.05. This rate was found to be high in patients hospitalized in the pediatric service and intensive care unit. 67 out of 100 strains were found to be suspicious for ESBL by Disk Diffusion Test DDT. DDST and E-tests were applied as confirmatory tests. The sensitivity of the DDST and E tests was 100. Screening for ESBL in Klebsiella spp and other members of Enterobacteriaceae isolates is necessary to reduce further selection and spread of these increasingly broad-spectrum antimicrobial-resistant enteric pathogens.

 Son yıllarda antibiyotik direncinin artması konusu tüm dünyada önemli bir sorun haline gelmiştir. Amacımız, Klebsiella spp izolatlarında antimikrobiyal direnç profilini ve Genişletilmiş Spektrumlu Beta- Laktamaz ESBL oranlarını belirleyerek bilinçsiz antibiyotik kullanımı sonucunda multi-dirençli izolatlarda kademeli artışı önlemek ve böylece enfeksiyonların daha hızlı etkin tedavisine katkıda bulunmaktır. Çeşitli klinik örneklerden toplam 100 Klebsiella türü izole edilmiş ve tanımlanmıştır. Antibiyotik duyarlılık testleri Kirby-Bauer yöntemi kullanılarak yapıldı. Genişletilmiş spektrumlu beta-laktamazların GSBL varlığı, Çift Disk Sinerji DDST ve E-test yöntemleri kullanılarak tespit edildi. GSBL üreten suşların oranları 6 K. oxytoca 46,1 ve 49 K. pneumoniae 56,3 idi. Bu suşlar 38 erişkin hastada 38, 17 pediatrik hastada 17 olarak bulundu. Sonuçlarımız istatistiksel olarak anlamlıydı p0,05. GSBL oranı 31 erkek hastada 31, 24 kadın hastada 24 idi ve cinsiyete göre bu fark anlamlı bulunmamıştır p0,05. Çocuk servisi ve yoğun bakım ünitesinde yatan hastalarda bu oran yüksek bulundu. Disk Difüzyon Testi DDT ile 100 suştan 67si ESBL açısından şüpheli bulundu. Doğrulayıcı testler olarak DDST ve E-testleri uygulandı. DDST ve E testlerinin duyarlılığı 100 idi. Klebsiella spp ve Enterobacteriaceae izolatlarının diğer üyelerinde ESBL taraması, giderek artan geniş spektrumlu antimikrobiyal dirençli enterik patojenlerin daha fazla seçimini ve yayılmasını azaltmak için gereklidir.

Makale detayları

Analysis of Extended Spectrum Beta Lactamase Frequency in Klebsiella spp Isolates

Yazarlar:

Mehmet Tevfik Bayraktar | Esma Ceylan | Bashar Ibrahım

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 1, pp.1-10

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287318

pISSN:

2146-247X

Klebsiella spp

Extended Spectrum Beta-lactamase (ESBL)

Double Disc Synergy Test

E-Test

Klebsiella spp

Genişletilmiş Spektrumlu Beta-laktamaz (ESBL)

Çift Disk Sinerji Testi

E-Testi

Sayı

Makale detayları

Yazarlar: Mehmet Tevfik Bayraktar | Esma Ceylan | Bashar Ibrahım

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Sağlık Kurumları Yönetimi

Dergi: Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 1, pp.1-10

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001287318

pISSN: 2146-247X

Sayı

Yazarlar:

Mehmet Tevfik Bayraktar | Esma Ceylan | Bashar Ibrahım

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 1, pp.1-10

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287318

pISSN:

2146-247X