Histological Evaluation of the Protective Role of β-glucan Against Cisplatin-Induced Hepatotoxicity

Burcu Demirel Yılmaz; Banu Eren; Dilek Sağır; Ayşe Başardı; Sevcan Mercan

Makale detayları

Histological Evaluation of the Protective Role of β-glucan Against Cisplatin-Induced Hepatotoxicity

Yazarlar:

Burcu Demirel Yılmaz | Banu Eren | Dilek Sağır | Ayşe Başardı | Sevcan Mercan

Yayıncı:

Karamanoglu Mehmetbey University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü | Kanser Biyolojisi | Hayvan Hücresi ve Moleküler Biyoloji

Dergi:

Karamanoğlu Mehmetbey Üniversitesi Mühendislik ve Doğa Bilimleri Dergisi Vol.6 Issue 1, pp.38-43

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001310428

pISSN:

2687-5071

Anahtar kelimeler:

Özet:

 Cisplatin is a commonly used chemotherapeutic agent in the treatment of many cancers. The most important dose-limiting side effect is hepatotoxicity. Some studies have shown that antioxidant treatment with cisplatin reduces the toxic effect. In the present study, we were aimed to investigate the protective effects of antioxidant β-glucan on histological injury caused by cisplatin treatment in the liver. Wistar rats were randomly divided into three groups according to time of sacrifice, 7th day and 14th day n20 rats each. Both groups were then divided into four sub-groups Control, Cisplatin 10 mgkg bw, β-glucan 100 mgkg bw and cisplatinβ-glucan n5 in each group. The rats were sacrificed at the 7th day and 14th day after the last injection. The liver sections were evaluated under a light microscope after the histological procedure. Histological injury caused by cisplatin in different days were evaluated as as sinusoidal congestion, hydropic degeneration, disorganization of hepatic cords, and mononuclear cellular infiltration in liver. When β-glucan was administered with cisplatin, it was determined that cellular damage caused by cisplatin decreased considerably in the liver in the different days groups. The light microscopic examination showed that the antioxidant beta-glucan protects against hepatotoxicity caused by cisplatin with its free radical scavenging effect. In conclusion, β-glucan may improve patients quality of life by reducing cisplatins toxicity on the liver.

 Cisplatin is a commonly used chemotherapeutic agent in the treatment of many cancers. The most important dose-limiting side effect is hepatotoxicity. Some studies have shown that antioxidant treatment with cisplatin reduces the toxic effect. In the present study, we were aimed to investigate the protective effects of antioxidant β-glucan on histological injury caused by cisplatin treatment in the liver. Wistar rats were randomly divided into three groups according to time of sacrifice, 7th day and 14th day n20 rats each. Both groups were then divided into four sub-groups Control, Cisplatin 10 mgkg bw, β-glucan 100 mgkg bw and cisplatinβ-glucan n5 in each group. The rats were sacrificed at the 7th day and 14th day after the last injection. The liver sections were evaluated under a light microscope after the histological procedure. Histological injury caused by cisplatin in different days were evaluated as as sinusoidal congestion, hydropic degeneration, disorganization of hepatic cords, and mononuclear cellular infiltration in liver. When β-glucan was administered with cisplatin, it was determined that cellular damage caused by cisplatin decreased considerably in the liver in the different days groups. The light microscopic examination showed that the antioxidant beta-glucan protects against hepatotoxicity caused by cisplatin with its free radical scavenging effect. In conclusion, β-glucan may improve patients quality of life by reducing cisplatins toxicity on the liver.

 Cisplatin is a commonly used chemotherapeutic agent in the treatment of many cancers. The most important dose-limiting side effect is hepatotoxicity. Some studies have shown that antioxidant treatment with cisplatin reduces the toxic effect. In the present study, we were aimed to investigate the protective effects of antioxidant β-glucan on histological injury caused by cisplatin treatment in the liver. Wistar rats were randomly divided into three groups according to time of sacrifice, 7th day and 14th day n20 rats each. Both groups were then divided into four sub-groups Control, Cisplatin 10 mgkg bw, β-glucan 100 mgkg bw and cisplatinβ-glucan n5 in each group. The rats were sacrificed at the 7th day and 14th day after the last injection. The liver sections were evaluated under a light microscope after the histological procedure. Histological injury caused by cisplatin in different days were evaluated as as sinusoidal congestion, hydropic degeneration, disorganization of hepatic cords, and mononuclear cellular infiltration in liver. When β-glucan was administered with cisplatin, it was determined that cellular damage caused by cisplatin decreased considerably in the liver in the different days groups. The light microscopic examination showed that the antioxidant beta-glucan protects against hepatotoxicity caused by cisplatin with its free radical scavenging effect. In conclusion, β-glucan may improve patients quality of life by reducing cisplatins toxicity on the liver.

 Sisplatin pek çok kanserin tedavisinde sıklıkla kullanılan kemoterapötik bir ajandır. En önemli doz sınırlayıcı yan etkisi hepatotoksisitedir. Bu çalışmada, sisplatin tedavisinin karaciğerde neden olduğu histolojik hasar üzerinde antioksidan β-glukanın etkilerinin araştırılması amaçlandı. Wistar ratlar sacrifiye edilecek zamana göre rastgele dört gruba ayrıldılar. Bu gruplar 1. gün, 2. gün, 7. ve 14. gün n20 her grupta 20 rat olarak belirlendi. Her grupta kendi içinde Kontrol, Sisplatin 10 mgkg, β-glukan 100 mgkg ve Sisplatinβ-glukan n5 her grupta olmak üzere dört alt gruba ayrıldı. Ratlar son enjeksiyondan sonraki 1. gün, 2. gün, 7. gün ve 14. günde sakrifiye edildi. Histolojik prosedürün ardından karaciğer kesitleri ışık mikroskobik incelendi. Sisplatinin farklı günlerde oluşturduğu histolojik hasar sinüzoidal konjesyon, hidropik dejenerasyon, hepatik kordlarda düzensizlik ve karaciğerde mononükleer hücre infiltrasyonu olarak değerlendirildi. Sisplatin ile birlikte β-glukan uygulandığında farklı gün gruplarında sisplatinin karaciğerde neden olduğu hücresel hasarın önemli ölçüde azaldığı belirlendi. Işık mikroskobik incelemede antioksidan β-glukanın serbest radikal temizleyici etkisi ile sisplatinin neden olduğu hepatotoksisiteye karşı koruma sağladığı görüldü. Sonuç olarak β-glukan, sisplatinin karaciğer üzerindeki toksisitesini azaltarak hastaların yaşam kalitesini arttırabilir.

Makale detayları

Histological Evaluation of the Protective Role of β-glucan Against Cisplatin-Induced Hepatotoxicity

Yazarlar:

Burcu Demirel Yılmaz | Banu Eren | Dilek Sağır | Ayşe Başardı | Sevcan Mercan

Yayıncı:

Karamanoglu Mehmetbey University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü | Kanser Biyolojisi | Hayvan Hücresi ve Moleküler Biyoloji

Dergi:

Karamanoğlu Mehmetbey Üniversitesi Mühendislik ve Doğa Bilimleri Dergisi Vol.6 Issue 1, pp.38-43

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001310428

pISSN:

2687-5071

Cisplatin

β-glucan

liver

histopathologySisplatin

β-glukan

karaciğer

histopatoloji

Sayı

Makale detayları

Yazarlar: Burcu Demirel Yılmaz | Banu Eren | Dilek Sağır | Ayşe Başardı | Sevcan Mercan

Yayıncı: Karamanoglu Mehmetbey University

Ana Başlıklar: Hücre Gelişimi | Proliferasyon ve Ölümü | Kanser Biyolojisi | Hayvan Hücresi ve Moleküler Biyoloji

Dergi: Karamanoğlu Mehmetbey Üniversitesi Mühendislik ve Doğa Bilimleri Dergisi Vol.6 Issue 1, pp.38-43

Belge Tipi: Makale

Yayın Tarihi: 6/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001310428

pISSN: 2687-5071

Sayı

Yazarlar:

Burcu Demirel Yılmaz | Banu Eren | Dilek Sağır | Ayşe Başardı | Sevcan Mercan

Yayıncı:

Karamanoglu Mehmetbey University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü | Kanser Biyolojisi | Hayvan Hücresi ve Moleküler Biyoloji

Dergi:

Karamanoğlu Mehmetbey Üniversitesi Mühendislik ve Doğa Bilimleri Dergisi Vol.6 Issue 1, pp.38-43

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001310428

pISSN:

2687-5071