RP-HPLC method development and validation for quantification of letrozole solid lipid nanoparticle

N.A.

Makale detayları

RP-HPLC method development and validation for quantification of letrozole solid lipid nanoparticle

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Sciences

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.421-430

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346349

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 A sensitive, simple, rapid, stability-indicating reverse-phase high-pressure liquid chromatographic method was developed for the estimation of letrozole in solid lipid nanoparticles. The developed method was validated as per the International Council of Harmonization ICH guideline concerning system suitability, linearity, accuracy, precision, specificity, robustness, and range. The chromatographic separation was achieved on the Zorbax C18 column 250 x 4.6 mm ID; 5.0 µm particle size. Isocratic elution was performed using methanol: 0.1 orthophosphoric acid 60:40 at 0.7 mLmin flow rate. The detection was done using a UV-Vis detector at 240 nm. Linearity for the analytical method was observed at the concentration range of 10 to 50 µgmL having a correlation coefficient of 0.999. The method was precise, accurate with a relative standard deviation RSD  2.0. The developed validated analytical method can be used as a quality control tool for the quantitative estimation of letrozole in a novel formulated solid lipid nanoparticle.

 A sensitive, simple, rapid, stability-indicating reverse-phase high-pressure liquid chromatographic method was developed for the estimation of letrozole in solid lipid nanoparticles. The developed method was validated as per the International Council of Harmonization ICH guideline concerning system suitability, linearity, accuracy, precision, specificity, robustness, and range. The chromatographic separation was achieved on the Zorbax C18 column 250 x 4.6 mm ID; 5.0 µm particle size. Isocratic elution was performed using methanol: 0.1 orthophosphoric acid 60:40 at 0.7 mLmin flow rate. The detection was done using a UV-Vis detector at 240 nm. Linearity for the analytical method was observed at the concentration range of 10 to 50 µgmL having a correlation coefficient of 0.999. The method was precise, accurate with a relative standard deviation RSD  2.0. The developed validated analytical method can be used as a quality control tool for the quantitative estimation of letrozole in a novel formulated solid lipid nanoparticle.

 A sensitive, simple, rapid, stability-indicating reverse-phase high-pressure liquid chromatographic method was developed for the estimation of letrozole in solid lipid nanoparticles. The developed method was validated as per the International Council of Harmonization ICH guideline concerning system suitability, linearity, accuracy, precision, specificity, robustness, and range. The chromatographic separation was achieved on the Zorbax C18 column 250 x 4.6 mm ID; 5.0 µm particle size. Isocratic elution was performed using methanol: 0.1 orthophosphoric acid 60:40 at 0.7 mLmin flow rate. The detection was done using a UV-Vis detector at 240 nm. Linearity for the analytical method was observed at the concentration range of 10 to 50 µgmL having a correlation coefficient of 0.999. The method was precise, accurate with a relative standard deviation RSD  2.0. The developed validated analytical method can be used as a quality control tool for the quantitative estimation of letrozole in a novel formulated solid lipid nanoparticle.

 A sensitive, simple, rapid, stability-indicating reverse-phase high-pressure liquid chromatographic method was developed for the estimation of letrozole in solid lipid nanoparticles. The developed method was validated as per the International Council of Harmonization ICH guideline concerning system suitability, linearity, accuracy, precision, specificity, robustness, and range. The chromatographic separation was achieved on the Zorbax C18 column 250 x 4.6 mm ID; 5.0 µm particle size. Isocratic elution was performed using methanol: 0.1 orthophosphoric acid 60:40 at 0.7 mLmin flow rate. The detection was done using a UV-Vis detector at 240 nm. Linearity for the analytical method was observed at the concentration range of 10 to 50 µgmL having a correlation coefficient of 0.999. The method was precise, accurate with a relative standard deviation RSD  2.0. The developed validated analytical method can be used as a quality control tool for the quantitative estimation of letrozole in a novel formulated solid lipid nanoparticle.

Makale detayları

RP-HPLC method development and validation for quantification of letrozole solid lipid nanoparticle

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Sciences

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.421-430

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346349

pISSN:

2630-6344

Letrozole

RP-HPLC

Solid lipid nanoparticle

ICH

Validation

Sayı

Makale detayları

RP-HPLC method development and validation for quantification of letrozole solid lipid nanoparticle

Yazarlar: N.A.

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Sciences

Dergi: Journal of Research in Pharmacy Vol.26 Issue 2, pp.421-430

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346349

pISSN: 2630-6344

Letrozole

RP-HPLC

Solid lipid nanoparticle

ICH

Validation

Sayı

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Sciences

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.421-430

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346349

pISSN:

2630-6344