Identification and Histopathology of Staphylococcus warneri in Rainbow Trout (Oncorhynchus mykiss)

Öznur Diler; Hasan Emre Yılmaz; İfakat Tulay Çağatay; Mevlüt Nazıroğlu; Öznur Özil; Şeydanur Kan

Makale detayları

Identification and Histopathology of Staphylococcus warneri in Rainbow Trout (Oncorhynchus mykiss)

Yazarlar:

Öznur Diler | Hasan Emre Yılmaz | İfakat Tulay Çağatay | Mevlüt Nazıroğlu | Öznur Özil | Şeydanur Kan

Yayıncı:

Isparta University of Applied Sciences

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Acta Aquatica Turcica Vol.19 Issue 3, pp.277-288

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001297336

pISSN:

2651-5474

Anahtar kelimeler:

Özet:

 This study was conducted to identify the disease that caused mortality in rainbow trout Oncorhynchus mykiss farms in the Aegean and Mediterranean regions in March 2022. External examination of infected trout revealed bilateral exophthalmos, hemorrhage in the lower jaw, dropsy, anemia in the liver, yellow exudate accumulation in the digestive system, hemorrhage in the adipose tissue and anus area, and spleen enlargement. A Gram-positive bacterium, Staphylococcus warneri, was isolated from infected fishs liver and anterior kidney. S. warneri was identified using morphological features, some conventional biochemical tests, and molecular genetic methods utilizing the 16S rRNA and sodA gene regions. Histopathological examination showed infiltration in the liver tissue, hyperemia in the spleen tissue, and necrotic changes in the kidney tubular epithelium of infected fish. According to the disk diffusion method, doxycycline was determined to be the most sensitive antibiotic against the S. warneri isolate. Although S. warneri was isolated in previous studies on rainbow trout, its histopathological effects on organs were determined for the first time in this study.

 This study was conducted to identify the disease that caused mortality in rainbow trout Oncorhynchus mykiss farms in the Aegean and Mediterranean regions in March 2022. External examination of infected trout revealed bilateral exophthalmos, hemorrhage in the lower jaw, dropsy, anemia in the liver, yellow exudate accumulation in the digestive system, hemorrhage in the adipose tissue and anus area, and spleen enlargement. A Gram-positive bacterium, Staphylococcus warneri, was isolated from infected fishs liver and anterior kidney. S. warneri was identified using morphological features, some conventional biochemical tests, and molecular genetic methods utilizing the 16S rRNA and sodA gene regions. Histopathological examination showed infiltration in the liver tissue, hyperemia in the spleen tissue, and necrotic changes in the kidney tubular epithelium of infected fish. According to the disk diffusion method, doxycycline was determined to be the most sensitive antibiotic against the S. warneri isolate. Although S. warneri was isolated in previous studies on rainbow trout, its histopathological effects on organs were determined for the first time in this study.

 This study was conducted to identify the disease that caused mortality in rainbow trout Oncorhynchus mykiss farms in the Aegean and Mediterranean regions in March 2022. External examination of infected trout revealed bilateral exophthalmos, hemorrhage in the lower jaw, dropsy, anemia in the liver, yellow exudate accumulation in the digestive system, hemorrhage in the adipose tissue and anus area, and spleen enlargement. A Gram-positive bacterium, Staphylococcus warneri, was isolated from infected fishs liver and anterior kidney. S. warneri was identified using morphological features, some conventional biochemical tests, and molecular genetic methods utilizing the 16S rRNA and sodA gene regions. Histopathological examination showed infiltration in the liver tissue, hyperemia in the spleen tissue, and necrotic changes in the kidney tubular epithelium of infected fish. According to the disk diffusion method, doxycycline was determined to be the most sensitive antibiotic against the S. warneri isolate. Although S. warneri was isolated in previous studies on rainbow trout, its histopathological effects on organs were determined for the first time in this study.

 Bu çalışma, Ege ve Akdeniz bölgesinde bulunan gökkuşağı alabalığı Oncorhynchus mykiss çiftliklerinde Mart 2022 tarihinde mortaliteye neden olan hastalığın tanımlanması amacıyla yürütülmüştür. Enfekte alabalıkların dış incelemelerinde gözlerde bilateral ekzoftalmus, alt çenede kanama, dropsi, internal olarak karaciğerde anemi, sindirim sisteminde sarı eksüdat birikimi, yağ dokusunda ve anüs bölgesinde kanama, dalakta büyüme görüldü. Enfekte balıkların karaciğer ve ön böbreğinden Gram-pozitif bir bakteri olan Staphylococcus warneri izole edildi. S. warneri, morfolojik özellikleri, bazı geleneksel testler ve 16S rRNA ve sodA gen bölgeleri kullanılarak moleküler genetik yöntemler yardımıyla teşhis edildi. Histopatolojik incelemelerde enfekte balıkların karaciğer dokusunda infiltrasyon, dalak dokusunda hiperemi ve böbrek tübül epitelinde nekrotik değişiklikler saptandı. Disk difüzyon yöntemine göre S. warneri izolatının en duyarlı olduğu antibiyotiğin doxycycline olduğu belirlendi. Gökkuşağı alabalıklarında yapılan daha önceki çalışmalarda S. warneri türü izole edilmekle birlikte bu çalışma ile ilk kez S. warneri türünün organlardaki histopatolojik etkileri belirlenmiştir.

Makale detayları

Identification and Histopathology of Staphylococcus warneri in Rainbow Trout (Oncorhynchus mykiss)

Yazarlar:

Öznur Diler | Hasan Emre Yılmaz | İfakat Tulay Çağatay | Mevlüt Nazıroğlu | Öznur Özil | Şeydanur Kan

Yayıncı:

Isparta University of Applied Sciences

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Acta Aquatica Turcica Vol.19 Issue 3, pp.277-288

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001297336

pISSN:

2651-5474

Rainbow trout (Oncorhynchus mykiss)

Staphylococcus warneri

16 S rRNA

sodA gene

HistopathologyGökkuşağı alabalığı (Oncorhynchus mykiss)

Staphylococcus warneri

16 S rRNA

sodA geni

Histopatoloji

Sayı

Makale detayları

Yazarlar: Öznur Diler | Hasan Emre Yılmaz | İfakat Tulay Çağatay | Mevlüt Nazıroğlu | Öznur Özil | Şeydanur Kan

Yayıncı: Isparta University of Applied Sciences

Ana Başlıklar: Yapısal Biyoloji

Dergi: Acta Aquatica Turcica Vol.19 Issue 3, pp.277-288

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001297336

pISSN: 2651-5474

Sayı

Yazarlar:

Öznur Diler | Hasan Emre Yılmaz | İfakat Tulay Çağatay | Mevlüt Nazıroğlu | Öznur Özil | Şeydanur Kan

Yayıncı:

Isparta University of Applied Sciences

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Acta Aquatica Turcica Vol.19 Issue 3, pp.277-288

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001297336

pISSN:

2651-5474