Effects of serum starvation and ionomycin activation on the development of somatic cell nuclear transfer embryos in sheep

Kamber DEMİR; Serhat PABUCCUOĞLU; Ümüt CİRİT; Mithat EVECEN; Elif KARAMAN; Özen Banu ÖZDAŞ; Serhat ALKAN; Hatem ATALLA; Sema BİRLER

Makale detayları

Effects of serum starvation and ionomycin activation on the development of somatic cell nuclear transfer embryos in sheep

Yazarlar:

Kamber DEMİR | Serhat PABUCCUOĞLU | Ümüt CİRİT | Mithat EVECEN | Elif KARAMAN | Özen Banu ÖZDAŞ | Serhat ALKAN | Hatem ATALLA | Sema BİRLER

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 1, pp.37-42

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000849693

Özet:

 

Synchronization of donor cells and activation of the reconstructed oocytes are important factors affecting the successrate in somatic cell cloning. In this study, it was aimed to investigate the effects of serum starvation in donor cell synchronization andionomycin treatment in the activation of reconstructed oocytes after somatic cell nuclear transfer in Kıvırcık sheep. Cumulus cells wereobtained from a slaughtered sheep ovaries and used as donor cells after serum starvation for 4 days (0.5% FCS; SS) or without serumstarvation (10% FCS; S). After reconstruction, oocytes were activated by ionomycin for 5 min plus 6-dimethylaminopurine for 3 h (I+)or only with 6-dimethylaminopurine for 3 h (I-). All cleaved embryos (n= 44) at the second day of in vitro culture were transferred intosynchronized recipient ewes (n= 10). Cleavage rates of the embryos were 37.3, 44.1, 34.6 and 44.7% in SS/I+, S/I+, SS/I- and S/Igroups, respectively. Recipient ewes had serum progesterone levels >1 ng/ml at 18th day were 33.3, 50.0, 50.0 and 100.0%, respectively.Only one pregnancy in the S/I- group continued after 40 days however the cloned lamb (7.1%, regarding to embryos transferred) died10 days before term due to a maternal problem (uterine torsion). The results of this study reveal that somatic cell synchronization byserum starvation and ionomycin treatment for the activation of oocytes can be omitted for the success of somatic cell nuclear transferin sheep  .

Makale detayları

Effects of serum starvation and ionomycin activation on the development of somatic cell nuclear transfer embryos in sheep

Yazarlar: Kamber DEMİR | Serhat PABUCCUOĞLU | Ümüt CİRİT | Mithat EVECEN | Elif KARAMAN | Özen Banu ÖZDAŞ | Serhat ALKAN | Hatem ATALLA | Sema BİRLER

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 1, pp.37-42

Belge Tipi: Makale

Yayın Tarihi: 12/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000849693

Sayı

Yazarlar:

Kamber DEMİR | Serhat PABUCCUOĞLU | Ümüt CİRİT | Mithat EVECEN | Elif KARAMAN | Özen Banu ÖZDAŞ | Serhat ALKAN | Hatem ATALLA | Sema BİRLER

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 1, pp.37-42

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000849693