LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION

N. Akev*, A. Can* R. YANARDAĞ**

Makale detayları

LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION

Yazarlar:

N. Akev, A. Can R. YANARDAĞ**

Yayıncı:

Istanbul University

Ana Başlıklar:

Farmakoloji ve terapötik

Dergi:

Journal of Faculty of Pharmacy of Istanbul University 1995 Vol.31 Issue 0, pp.1-10

Belge Tipi:

Makale

Yayın Tarihi:

9/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000513623

Sıklık:

Aylık

eISSN:

2587-2087

Özet:

 

Three electrophoretically distinct aspartate minotransferase isoenzymes
named AAT-1, AAT-2and AAT-3, were separated from lentil (Lens culinaris
Medik.) seeds by extraction, ammonium sulphate precipitation, hydroxylapatite
and DEAE-cellulose column chromatographies. AAT-1 was purified 228,
AAT-2,42 and AAT-3, 3.8 fold. The isoenzymes were examined by means of
polyacrylamide gel electrophoresis.Owing to its key role in general metabolism, aspartate adotransferase (AAT, E.C. 2.6.1.1.) which was formerly called glutamic oxaloacetic transaminase (GOT), occurs widely in nature. In animals, cytbsolic and rnitochon+al forms of AAT have been characterized and purified (1-4). AAT has also been studied in various plants (5, 6) and different isoeozyrnic forms have been r&-, ported. The enzyme has been extensively characterized in wheat germ (7,8), cauliflower (9), cotton seeds (lo), pea (ll), soybean (12-14), oat (15), lupine (16) and alfalfa (17,18).

 


Elektroforetik balurndan far& ii~ aspartat aminotransferaz izoenzimi yegil mercimek (Lens culinaris Medik.) tohudarmdan ekstraksiyon , amonyum sulfatla goktiirme, hidroksilapatit ve DEAE-seluloz sutun kromatografisi yontemleri kullanllarak aynld~. AAT-1, AAT-2 ve AAT-3 olarak adlandlrilan bu izoenzimler, slras~yla 228, 42 ve 3.8 kez saflagtlrdd~. Izoenzimler poliakrilamid jelelektroforezi ile incelendi.

Elektroforetik balurndan far& ii~ aspartat aminotransferaz izoenzimi yegil mercimek (Lens culinaris Medik.) tohudarmdan ekstraksiyon , amonyum sulfatla goktiirme, hidroksilapatit ve DEAE-seluloz sutun kromatografisi yontemleri kullanllarak aynld~. AAT-1, AAT-2 ve AAT-3 olarak adlandlrilan bu izoenzimler, slras~yla 228, 42 ve 3.8 kez saflagtlrdd~. Izoenzimler poliakrilamid jelelektroforezi ile incelendi.

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Makale detayları

LENTIL SEED ASPARTATE APvIINOTRANSFERASE ISOENZYMES I. ISOLATION and PARTIAL PURIFICATION

Yazarlar: N. Akev*, A. Can* R. YANARDAĞ**

Yayıncı: Istanbul University

Ana Başlıklar: Farmakoloji ve terapötik

Dergi: Journal of Faculty of Pharmacy of Istanbul University 1995 Vol.31 Issue 0, pp.1-10

Belge Tipi: Makale

Yayın Tarihi: 9/2015

Belge Dili: İngilizce

Document ID (Org No.): 2000513623

Sıklık: Aylık

eISSN: 2587-2087

Sayı

Yazarlar:

N. Akev, A. Can R. YANARDAĞ**

Yayıncı:

Istanbul University

Ana Başlıklar:

Farmakoloji ve terapötik

Dergi:

Journal of Faculty of Pharmacy of Istanbul University 1995 Vol.31 Issue 0, pp.1-10

Belge Tipi:

Makale

Yayın Tarihi:

9/2015

Belge Dili:

İngilizce

Document ID (Org No.):

2000513623

Sıklık:

Aylık

eISSN:

2587-2087