Effects of I-131 ablation/metastasis treatment on DNA damage parameters in patients with differentiated thyroid cancer: a pilot study

Emine Kılıç Toprak; Tarık Şengöz; Olga Yaylalı; Özgen Kılıç Erkek; Yasin Özdemir; Burak Oymak; Hande Şenol; Z. Melek Küçükatay; Vural Küçükatay; Doğangün Yüksel

Makale detayları

Effects of I-131 ablation/metastasis treatment on DNA damage parameters in patients with differentiated thyroid cancer: a pilot study

Yazarlar:

Emine Kılıç Toprak | Tarık Şengöz | Olga Yaylalı | Özgen Kılıç Erkek | Yasin Özdemir | Burak Oymak | Hande Şenol | Z. Melek Küçükatay | Vural Küçükatay | Doğangün Yüksel

Yayıncı:

Cukurova University

Ana Başlıklar:

Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi:

Cukurova Medical Journal Vol.50 Issue 3, pp.609-616

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357830

pISSN:

2602-3032

Anahtar kelimeler:

Özet:

 Purpose: The purpose of this study was to ascertain how radioactive iodine therapy RAIT. affected the DNA damage in the lymphocytes of differentiated thyroid cancer DTC patients. Materials and Methods: Our study included 21 DTC patients in total, with a mean age of 45.0910.02 years. Six 28.6 of the 21 patients had a low dose of RAIT 30 mCi, eleven 52.4 received a moderate dose of ablation 31 to 100 mCi, and four 19 received a high dose 100 mCi. Before, one week, and six months following treatment, venous blood samples were obtained from each patient. DNA damage was evaluated using the COMET assay. For a quantitative analysis of DNA damage, a number of characteristics were assessed, including head length, tail length, head intensity, tail intensity, and tail moment. Results: Compared to before treatment 23.839.82, tail intensity levels were considerably lower after treatment 1st week and 6th months 18.306.48 vs. 18.065.58. Following therapy 1st week and 6th months, the head intensity values were statistically substantially higher 81.696.48 vs. 81.935.58 than they were prior to treatment 76.169.82. Six months after therapy, the tail moment was lower than it was before treatment 5.242.51 vs. 8.043.86. Conclusion: Treatment has a beneficial effect on peripheral blood cells, as seen by the increase in head intensity and decrease in tail intensity and moment after treatment. By triggering repair mechanisms, RAIT may have activated protective effects on the DNA of lymphocytes.

 Purpose: The purpose of this study was to ascertain how radioactive iodine therapy RAIT. affected the DNA damage in the lymphocytes of differentiated thyroid cancer DTC patients. Materials and Methods: Our study included 21 DTC patients in total, with a mean age of 45.0910.02 years. Six 28.6 of the 21 patients had a low dose of RAIT 30 mCi, eleven 52.4 received a moderate dose of ablation 31 to 100 mCi, and four 19 received a high dose 100 mCi. Before, one week, and six months following treatment, venous blood samples were obtained from each patient. DNA damage was evaluated using the COMET assay. For a quantitative analysis of DNA damage, a number of characteristics were assessed, including head length, tail length, head intensity, tail intensity, and tail moment. Results: Compared to before treatment 23.839.82, tail intensity levels were considerably lower after treatment 1st week and 6th months 18.306.48 vs. 18.065.58. Following therapy 1st week and 6th months, the head intensity values were statistically substantially higher 81.696.48 vs. 81.935.58 than they were prior to treatment 76.169.82. Six months after therapy, the tail moment was lower than it was before treatment 5.242.51 vs. 8.043.86. Conclusion: Treatment has a beneficial effect on peripheral blood cells, as seen by the increase in head intensity and decrease in tail intensity and moment after treatment. By triggering repair mechanisms, RAIT may have activated protective effects on the DNA of lymphocytes.

 Purpose: The purpose of this study was to ascertain how radioactive iodine therapy RAIT. affected the DNA damage in the lymphocytes of differentiated thyroid cancer DTC patients. Materials and Methods: Our study included 21 DTC patients in total, with a mean age of 45.0910.02 years. Six 28.6 of the 21 patients had a low dose of RAIT 30 mCi, eleven 52.4 received a moderate dose of ablation 31 to 100 mCi, and four 19 received a high dose 100 mCi. Before, one week, and six months following treatment, venous blood samples were obtained from each patient. DNA damage was evaluated using the COMET assay. For a quantitative analysis of DNA damage, a number of characteristics were assessed, including head length, tail length, head intensity, tail intensity, and tail moment. Results: Compared to before treatment 23.839.82, tail intensity levels were considerably lower after treatment 1st week and 6th months 18.306.48 vs. 18.065.58. Following therapy 1st week and 6th months, the head intensity values were statistically substantially higher 81.696.48 vs. 81.935.58 than they were prior to treatment 76.169.82. Six months after therapy, the tail moment was lower than it was before treatment 5.242.51 vs. 8.043.86. Conclusion: Treatment has a beneficial effect on peripheral blood cells, as seen by the increase in head intensity and decrease in tail intensity and moment after treatment. By triggering repair mechanisms, RAIT may have activated protective effects on the DNA of lymphocytes.

 Amaç: Bu çalışmanın amacı, radyoaktif iyot tedavisinin RAIT farklılaşmış tiroid kanseri DTC hastalarının lenfositlerindeki DNA hasarını nasıl etkilediğini belirlemekti. Gereç ve Yöntem: Çalışmamıza toplam 21 DTC hastası dahil edildi ve yaş ortalamaları 45.0910.02 yıldı. 21 hastanın altısı 28.6 düşük doz RAIT 30 mCi aldı, on biri 52.4 orta doz ablasyon 31 ila 100 mCi aldı ve dördü 19 yüksek doz 100 mCi aldı. Tedaviden önce, bir hafta ve altı ay sonra her hastadan venöz kan örnekleri alındı. DNA hasarı COMET testi kullanılarak değerlendirildi. DNA hasarının kantitatif analizi için baş uzunluğu, kuyruk uzunluğu, baş yoğunluğu, kuyruk yoğunluğu ve kuyruk momenti dahil olmak üzere çeşitli parametreler değerlendirildi. Bulgular: Kuyruk yoğunluğu değerleri tedaviden sonra 1 hafta ve 6 ay tedavi öncesine 23,83  9,82 kıyasla önemli ölçüde daha düşüktü 18,30  6,48e karşı 18,06  5,58. Baş yoğunluğu değerleri tedaviden sonra 1. hafta ve 6. ay 81.696.48e karşı 81.935.58 tedavi öncesine 76.169.82 göre istatistiksel olarak anlamlı derecede daha yüksekti. Kuyruk momenti tedaviden 6 ay sonra tedavi öncesine göre daha düşüktü 5.242.51e karşı 8.043.86. Sonuç: Tedaviden sonra baş yoğunluğunda artış ve kuyruk yoğunluğunda ve momentinde azalma, tedavinin periferik kan hücreleri üzerinde olumlu bir etkiye sahip olduğunu göstermiştir. RAITin onarım mekanizmalarını aktive ederek lenfosit DNAsı üzerinde koruyucu etkileri aktive etmiş olabileceği düşünülebilir.

Makale detayları

Effects of I-131 ablation/metastasis treatment on DNA damage parameters in patients with differentiated thyroid cancer: a pilot study

Yazarlar:

Emine Kılıç Toprak | Tarık Şengöz | Olga Yaylalı | Özgen Kılıç Erkek | Yasin Özdemir | Burak Oymak | Hande Şenol | Z. Melek Küçükatay | Vural Küçükatay | Doğangün Yüksel

Yayıncı:

Cukurova University

Ana Başlıklar:

Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi:

Cukurova Medical Journal Vol.50 Issue 3, pp.609-616

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357830

pISSN:

2602-3032

Differentiated thyroid cancer

I-131

radioactive iodine theraphy

DNA damage

COMET assayFarklılaşmış tiroid kanseri

I-131

radyoaktif iyot tedavisi

DNA hasarı

COMET analizi

Sayı

Makale detayları

Yazarlar: Emine Kılıç Toprak | Tarık Şengöz | Olga Yaylalı | Özgen Kılıç Erkek | Yasin Özdemir | Burak Oymak | Hande Şenol | Z. Melek Küçükatay | Vural Küçükatay | Doğangün Yüksel

Yayıncı: Cukurova University

Ana Başlıklar: Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi: Cukurova Medical Journal Vol.50 Issue 3, pp.609-616

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001357830

pISSN: 2602-3032

Sayı

Yazarlar:

Emine Kılıç Toprak | Tarık Şengöz | Olga Yaylalı | Özgen Kılıç Erkek | Yasin Özdemir | Burak Oymak | Hande Şenol | Z. Melek Küçükatay | Vural Küçükatay | Doğangün Yüksel

Yayıncı:

Cukurova University

Ana Başlıklar:

Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Dergi:

Cukurova Medical Journal Vol.50 Issue 3, pp.609-616

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357830

pISSN:

2602-3032