Investigation Effects of Some Plant Hormones and Vitamins on Growth in Kanamycin-Resistant Tomato Varieties

Yunus Emre Uslu; İsmail Bezirganoglu

Makale detayları

Investigation Effects of Some Plant Hormones and Vitamins on Growth in Kanamycin-Resistant Tomato Varieties

Yazarlar:

Yunus Emre Uslu | İsmail Bezirganoglu

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Plant Developmental and Reproductive Biology | Plant Biotechnology in Agriculture

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 2, pp.426-432

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345246

pISSN:

2148-3647

Anahtar kelimeler:

Özet:

 Tomato is a widely consumed vegetable around the world and has a significant economic impact on agricultural production. However, various challenges in current agricultural practices can affect tomato production. To overcome these challenges and create a sustainable agricultural model, research focusing on tomato plant tissue culture is gaining importance. In this study, the neomycin phosphotransferase NPTII reporter gene was transferred to the Lycopersicon esculentum cLN1558A line through A. tumefaciens LBA4404. The effects of phenolic compounds and growth regulators used in tomato tissue culture were investigated. A balanced concentration of 1 mg ml-1 6-benzyladenine BA and 1 mg ml-1 gibberellic acid GA3 was used for optimal shoot regeneration. For optimal root regeneration, 1 mg ml-1 indole-3-acetic acid IAA concentration was used. 200 μM acetosyringone was added to the culture medium to accelerate A. tumefaciens infection. As a result, it was confirmed that kanamycin resistance reached 50 in transgenic tomato shoots containing the NPTII gene. The efficiency of the phenolic compounds and growth regulators used increased by 12.

 Tomato is a widely consumed vegetable around the world and has a significant economic impact on agricultural production. However, various challenges in current agricultural practices can affect tomato production. To overcome these challenges and create a sustainable agricultural model, research focusing on tomato plant tissue culture is gaining importance. In this study, the neomycin phosphotransferase NPTII reporter gene was transferred to the Lycopersicon esculentum cLN1558A line through A. tumefaciens LBA4404. The effects of phenolic compounds and growth regulators used in tomato tissue culture were investigated. A balanced concentration of 1 mg ml-1 6-benzyladenine BA and 1 mg ml-1 gibberellic acid GA3 was used for optimal shoot regeneration. For optimal root regeneration, 1 mg ml-1 indole-3-acetic acid IAA concentration was used. 200 μM acetosyringone was added to the culture medium to accelerate A. tumefaciens infection. As a result, it was confirmed that kanamycin resistance reached 50 in transgenic tomato shoots containing the NPTII gene. The efficiency of the phenolic compounds and growth regulators used increased by 12.

 Tomato is a widely consumed vegetable around the world and has a significant economic impact on agricultural production. However, various challenges in current agricultural practices can affect tomato production. To overcome these challenges and create a sustainable agricultural model, research focusing on tomato plant tissue culture is gaining importance. In this study, the neomycin phosphotransferase NPTII reporter gene was transferred to the Lycopersicon esculentum cLN1558A line through A. tumefaciens LBA4404. The effects of phenolic compounds and growth regulators used in tomato tissue culture were investigated. A balanced concentration of 1 mg ml-1 6-benzyladenine BA and 1 mg ml-1 gibberellic acid GA3 was used for optimal shoot regeneration. For optimal root regeneration, 1 mg ml-1 indole-3-acetic acid IAA concentration was used. 200 μM acetosyringone was added to the culture medium to accelerate A. tumefaciens infection. As a result, it was confirmed that kanamycin resistance reached 50 in transgenic tomato shoots containing the NPTII gene. The efficiency of the phenolic compounds and growth regulators used increased by 12.

 Domates dünya çapında yaygın olarak tüketilen bir sebzedir ve tarımsal üretim üzerinde önemli bir ekonomik etkiye sahiptir. Ancak mevcut tarımsal uygulamalardaki çeşitli zorluklar domates üretimini etkileyebilmektedir. Bu zorlukların üstesinden gelmek ve sürdürülebilir bir tarım modeli oluşturmak için domates bitkisi doku kültürüne odaklanan araştırmalar önem kazanmaktadır. Bu çalışmada neomisin fosfotransferaz NPTII raportör geni, A. tumefaciens LBA4404 aracılığıyla Lycopersicon esculentum cLN1558A hattına aktarıldı. Domates doku kültüründe kullanılan fenolik bileşiklerin ve büyüme düzenleyicilerin etkileri araştırıldı. Optimum sürgün rejenerasyonu için dengeli bir 1 mg ml-1 6-benziladenin BA ve 1 mg ml-1 gibberellik asit GA3 konsantrasyonu kullanıldı. Optimum kök rejenerasyonu için 1 mg ml-1 indol-3-asetik asit IAA konsantrasyonu kullanıldı. A. tumefaciens enfeksiyonunu hızlandırmak için kültür ortamına 200 μM asetosiringon eklendi. Sonuç olarak NPTII genini içeren transgenik domates sürgünlerinde kanamisin direncinin 50ye ulaştığı doğrulandı. Kullanılan fenolik bileşiklerin ve büyüme düzenleyicilerin etkinliği 12 artmıştır.

Makale detayları

Investigation Effects of Some Plant Hormones and Vitamins on Growth in Kanamycin-Resistant Tomato Varieties

Yazarlar:

Yunus Emre Uslu | İsmail Bezirganoglu

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Plant Developmental and Reproductive Biology | Plant Biotechnology in Agriculture

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 2, pp.426-432

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345246

pISSN:

2148-3647

Giberellic acid

6-benzyladenine

NPTII

tomato tissue cultureGiberellik asit

6-benzyladenine

NPTII

domates doku kültürü

Sayı

Makale detayları

Yazarlar: Yunus Emre Uslu | İsmail Bezirganoglu

Yayıncı: Mevlüt AKÇURA

Ana Başlıklar: Plant Developmental and Reproductive Biology | Plant Biotechnology in Agriculture

Dergi: Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 2, pp.426-432

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001345246

pISSN: 2148-3647

Sayı

Yazarlar:

Yunus Emre Uslu | İsmail Bezirganoglu

Yayıncı:

Mevlüt AKÇURA

Ana Başlıklar:

Plant Developmental and Reproductive Biology | Plant Biotechnology in Agriculture

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.12 Issue 2, pp.426-432

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001345246

pISSN:

2148-3647