Identification, characterization, and expression profiling of salt-stress tolerant proton gradient regulator 5 (PGR5) in Gossypium arboreum

Muhammad SHAHID; Adil JAMAL; Beenish AFTAB; Bahaeldeen Babiker MOHAMED; Javed WATTOO; Muhammad Sarfraz KIANI; Bushra RASHID; Tayyab HUSNAIN

Makale detayları

Identification, characterization, and expression profiling of salt-stress tolerant proton gradient regulator 5 (PGR5) in Gossypium arboreum

Yazarlar:

Muhammad SHAHID | Adil JAMAL | Beenish AFTAB | Bahaeldeen Babiker MOHAMED | Javed WATTOO | Muhammad Sarfraz KIANI | Bushra RASHID | Tayyab HUSNAIN

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 4, pp.889-898

Belge Tipi:

Makale

Yayın Tarihi:

7/2016

Belge Dili:

İngilizce

Document ID (Org No.):

2000811633

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Salinity is among the significant abiotic stresses adversely affecting plant development and yield for a wide range of crops. For quantitative and qualitative expression studies of proton gradient regulator 5 (PGR5) under salt stress, differential display RT-PCR and rapid amplification of cDNA ends (RACE) were performed on salt-stress responsive Gossypium arboreum. Alignment of genomic and cDNA sequences revealed that the GPGR5 gene comprises a single open reading frame of 96 amino acids and contains no introns. Alignment of cotton GPGR5 complete amino acid sequence with PGR5 from other plants revealed the following identities: Gossypium raimondii (97%), Amaranthus hybridus (72%), Vitis vinifera (69%), Medicago truncatula (68%), Cucumis melo (62%), Arabidopsis thaliana (62%), Portulaca oleracea (61%), Portulaca grandiflora (60%), and Zea mays (55%). The expression profile was studied in different plant tissues (stem, leaf, and root) under the abiotic stresses salt, drought, and cold. The results showed a 7-fold increased expression of GPGR5 in leaf tissue in salt stress and almost no induction of transcription in root and stem tissues in salt stress. This gene has a good expression pattern under cold stress compared to salt and drought. In future studies, GPGR5 may be used as a prognostic marker for the development of abiotic-stress-tolerant plants.

Makale detayları

Identification, characterization, and expression profiling of salt-stress tolerant proton gradient regulator 5 (PGR5) in Gossypium arboreum

Yazarlar: Muhammad SHAHID | Adil JAMAL | Beenish AFTAB | Bahaeldeen Babiker MOHAMED | Javed WATTOO | Muhammad Sarfraz KIANI | Bushra RASHID | Tayyab HUSNAIN

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2016 Vol.40 Issue 4, pp.889-898

Belge Tipi: Makale

Yayın Tarihi: 7/2016

Belge Dili: İngilizce

Document ID (Org No.): 2000811633

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Muhammad SHAHID | Adil JAMAL | Beenish AFTAB | Bahaeldeen Babiker MOHAMED | Javed WATTOO | Muhammad Sarfraz KIANI | Bushra RASHID | Tayyab HUSNAIN

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 4, pp.889-898

Belge Tipi:

Makale

Yayın Tarihi:

7/2016

Belge Dili:

İngilizce

Document ID (Org No.):

2000811633

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152