Determination of CAST/MspI gene polymorphism in selected sheep breeds reared in Türkiye

Göknur Bayrak; Yasemin Gedik

Makale detayları

Determination of CAST/MspI gene polymorphism in selected sheep breeds reared in Türkiye

Yazarlar:

Göknur Bayrak | Yasemin Gedik

Yayıncı:

Akdeniz University

Ana Başlıklar:

Animal Science | Genetics and Biostatistics

Dergi:

Mediterranean Agricultural Sciences Vol.38 Issue 2, pp.103-108

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356483

pISSN:

2528-9675

Anahtar kelimeler:

Özet:

 Sheep breeding plays a crucial role in meeting the increasing demand for high-quality meat, milk, and wool. Traditional selection methods based on phenotypic traits have led to genetic improvement; however, progress remains slow for traits with low to moderate heritability. Marker-assisted selection MAS has emerged as a promising complementary approach, enabling faster genetic gains. Among candidate genes studied for MAS, the calpastatin CAST gene is notable for its association with meat yield and quality. This study aimed to identify CASTMspI gene polymorphisms in four sheep breeds reared in Türkiye Central Anatolian Merino-CAM, Pırlak-PRL, Romanov-RMV, and Suffolk-SFK and assess their potential use in MAS. A total of 176 individuals were genotyped using the PCR-RFLP method. All breeds in this study were found to be polymorphic for the CAST gene. The frequency of the M allele ranged from 0.67 in CAM to 0.76 in SFK. Genotype frequencies for MM ranged from 0.40 CAM to 0.71 SFK, for MN from 0.10 RMV and SFK to 0.54 CAM, and for NN from 0.06 CAM to 0.20 RMV. Significant deviations from Hardy-Weinberg equilibrium were observed in CAM, RMV, and SFK populations, but not in PRL. Observed heterozygosity ranged from 0.10 to 0.54 and expected heterozygosity from 0.37 to 0.44. The presence of all three genotypes and substantial genetic variation suggests that the CAST gene may be a valuable marker in MAS for these breeds. However, further association studies are required to confirm the relationship between CAST genotypes and economically significant traits related to meat production.

 Sheep breeding plays a crucial role in meeting the increasing demand for high-quality meat, milk, and wool. Traditional selection methods based on phenotypic traits have led to genetic improvement; however, progress remains slow for traits with low to moderate heritability. Marker-assisted selection MAS has emerged as a promising complementary approach, enabling faster genetic gains. Among candidate genes studied for MAS, the calpastatin CAST gene is notable for its association with meat yield and quality. This study aimed to identify CASTMspI gene polymorphisms in four sheep breeds reared in Türkiye Central Anatolian Merino-CAM, Pırlak-PRL, Romanov-RMV, and Suffolk-SFK and assess their potential use in MAS. A total of 176 individuals were genotyped using the PCR-RFLP method. All breeds in this study were found to be polymorphic for the CAST gene. The frequency of the M allele ranged from 0.67 in CAM to 0.76 in SFK. Genotype frequencies for MM ranged from 0.40 CAM to 0.71 SFK, for MN from 0.10 RMV and SFK to 0.54 CAM, and for NN from 0.06 CAM to 0.20 RMV. Significant deviations from Hardy-Weinberg equilibrium were observed in CAM, RMV, and SFK populations, but not in PRL. Observed heterozygosity ranged from 0.10 to 0.54 and expected heterozygosity from 0.37 to 0.44. The presence of all three genotypes and substantial genetic variation suggests that the CAST gene may be a valuable marker in MAS for these breeds. However, further association studies are required to confirm the relationship between CAST genotypes and economically significant traits related to meat production.

 Sheep breeding plays a crucial role in meeting the increasing demand for high-quality meat, milk, and wool. Traditional selection methods based on phenotypic traits have led to genetic improvement; however, progress remains slow for traits with low to moderate heritability. Marker-assisted selection MAS has emerged as a promising complementary approach, enabling faster genetic gains. Among candidate genes studied for MAS, the calpastatin CAST gene is notable for its association with meat yield and quality. This study aimed to identify CASTMspI gene polymorphisms in four sheep breeds reared in Türkiye Central Anatolian Merino-CAM, Pırlak-PRL, Romanov-RMV, and Suffolk-SFK and assess their potential use in MAS. A total of 176 individuals were genotyped using the PCR-RFLP method. All breeds in this study were found to be polymorphic for the CAST gene. The frequency of the M allele ranged from 0.67 in CAM to 0.76 in SFK. Genotype frequencies for MM ranged from 0.40 CAM to 0.71 SFK, for MN from 0.10 RMV and SFK to 0.54 CAM, and for NN from 0.06 CAM to 0.20 RMV. Significant deviations from Hardy-Weinberg equilibrium were observed in CAM, RMV, and SFK populations, but not in PRL. Observed heterozygosity ranged from 0.10 to 0.54 and expected heterozygosity from 0.37 to 0.44. The presence of all three genotypes and substantial genetic variation suggests that the CAST gene may be a valuable marker in MAS for these breeds. However, further association studies are required to confirm the relationship between CAST genotypes and economically significant traits related to meat production.

 Sheep breeding plays a crucial role in meeting the increasing demand for high-quality meat, milk, and wool. Traditional selection methods based on phenotypic traits have led to genetic improvement; however, progress remains slow for traits with low to moderate heritability. Marker-assisted selection MAS has emerged as a promising complementary approach, enabling faster genetic gains. Among candidate genes studied for MAS, the calpastatin CAST gene is notable for its association with meat yield and quality. This study aimed to identify CASTMspI gene polymorphisms in four sheep breeds reared in Türkiye Central Anatolian Merino-CAM, Pırlak-PRL, Romanov-RMV, and Suffolk-SFK and assess their potential use in MAS. A total of 176 individuals were genotyped using the PCR-RFLP method. All breeds in this study were found to be polymorphic for the CAST gene. The frequency of the M allele ranged from 0.67 in CAM to 0.76 in SFK. Genotype frequencies for MM ranged from 0.40 CAM to 0.71 SFK, for MN from 0.10 RMV and SFK to 0.54 CAM, and for NN from 0.06 CAM to 0.20 RMV. Significant deviations from Hardy-Weinberg equilibrium were observed in CAM, RMV, and SFK populations, but not in PRL. Observed heterozygosity ranged from 0.10 to 0.54 and expected heterozygosity from 0.37 to 0.44. The presence of all three genotypes and substantial genetic variation suggests that the CAST gene may be a valuable marker in MAS for these breeds. However, further association studies are required to confirm the relationship between CAST genotypes and economically significant traits related to meat production.

Makale detayları

Determination of CAST/MspI gene polymorphism in selected sheep breeds reared in Türkiye

Yazarlar:

Göknur Bayrak | Yasemin Gedik

Yayıncı:

Akdeniz University

Ana Başlıklar:

Animal Science | Genetics and Biostatistics

Dergi:

Mediterranean Agricultural Sciences Vol.38 Issue 2, pp.103-108

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356483

pISSN:

2528-9675

Calpastatin gene

MspI

Polymorphism

SheepCalpastatin gene

MspI

Polymorphism

Sheep

Sayı

Makale detayları

Yazarlar: Göknur Bayrak | Yasemin Gedik

Yayıncı: Akdeniz University

Ana Başlıklar: Animal Science | Genetics and Biostatistics

Dergi: Mediterranean Agricultural Sciences Vol.38 Issue 2, pp.103-108

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001356483

pISSN: 2528-9675

Sayı

Yazarlar:

Göknur Bayrak | Yasemin Gedik

Yayıncı:

Akdeniz University

Ana Başlıklar:

Animal Science | Genetics and Biostatistics

Dergi:

Mediterranean Agricultural Sciences Vol.38 Issue 2, pp.103-108

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356483

pISSN:

2528-9675