Assessment of Two Highly Polymorphic Barley Microsatellite Markers for Detecting Polymorphism in Wheat

E. Banu Büyükünal BAL; Mahinur S. AKKAYA

Makale detayları

Assessment of Two Highly Polymorphic Barley Microsatellite Markers for Detecting Polymorphism in Wheat

Yazarlar:

E. Banu Büyükünal BAL | Mahinur S. AKKAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 1, pp.9-12

Belge Tipi:

Makale

Yayın Tarihi:

12/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000813754

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Simple sequence repeat (SSR) DNA polymorphism is a PCR-based marker system, also known as the microsatellite marker system. SSRs are tandemly repeated 1 to 5 nucleotide units dispersed throughout eukaryotic genomes. They are considered one of the most powerful molecular markers for many diverse applications in genome related studies, e.g., DNA fingerprinting for discrimination of genotypes and genetic mapping. Locus specificity, co-dominant inheritance and easy detection with PCR amplifications are some of the favorable features of this marker system. On the other hand, isolation of SSR markers depends on time-and labor-consuming processes. The need to obtain sequence information for locus specific primer design is the major drawback. Therefore, the application of SSR markers in a cross-species manner would be valuable. In this study, two highly polymorphic barley SSR markers were used to test the cross transportability and polymorphism property of barley SSR primers on wheat genotypes. The results indicated that barley specific SSR primers could generate PCR amplification products on the wheat genome in the absence of polymorphism.

 

Simple sequence repeat (SSR) DNA polymorphism is a PCR-based marker system, also known as the microsatellite marker system. SSRs are tandemly repeated 1 to 5 nucleotide units dispersed throughout eukaryotic genomes. They are considered one of the most powerful molecular markers for many diverse applications in genome related studies, e.g., DNA fingerprinting for discrimination of genotypes and genetic mapping. Locus specificity, co-dominant inheritance and easy detection with PCR amplifications are some of the favorable features of this marker system. On the other hand, isolation of SSR markers depends on time-and labor-consuming processes. The need to obtain sequence information for locus specific primer design is the major drawback. Therefore, the application of SSR markers in a cross-species manner would be valuable. In this study, two highly polymorphic barley SSR markers were used to test the cross transportability and polymorphism property of barley SSR primers on wheat genotypes. The results indicated that barley specific SSR primers could generate PCR amplification products on the wheat genome in the absence of polymorphism.

Makale detayları

Assessment of Two Highly Polymorphic Barley Microsatellite Markers for Detecting Polymorphism in Wheat

Yazarlar:

E. Banu Büyükünal BAL | Mahinur S. AKKAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 1, pp.9-12

Belge Tipi:

Makale

Yayın Tarihi:

12/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000813754

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

SSR

microsatellite

DNA-based markers

barley and wheat.

Sayı

Makale detayları

Assessment of Two Highly Polymorphic Barley Microsatellite Markers for Detecting Polymorphism in Wheat

Yazarlar: E. Banu Büyükünal BAL | Mahinur S. AKKAYA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2002 Vol.26 Issue 1, pp.9-12

Belge Tipi: Makale

Yayın Tarihi: 12/2001

Belge Dili: Türkçe

Document ID (Org No.): 2000813754

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

SSR

microsatellite

DNA-based markers

barley and wheat.

Sayı

Yazarlar:

E. Banu Büyükünal BAL | Mahinur S. AKKAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2002 Vol.26 Issue 1, pp.9-12

Belge Tipi:

Makale

Yayın Tarihi:

12/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000813754

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152