Development of a biosensor platform based on ITO sheets modified with 3-glycidoxypropyltrimethoxysilane for early detection of TRAP1

Berfin VURAL; Meltem ÇALIŞKAN; Mustafa Kemal SEZGİNTÜRK

doi:https://doi.org/10.3906/kim-1909-53

Makale detayları

Development of a biosensor platform based on ITO sheets modified with 3-glycidoxypropyltrimethoxysilane for early detection of TRAP1

Yazarlar:

Berfin VURAL | Meltem ÇALIŞKAN | Mustafa Kemal SEZGİNTÜRK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2020 Vol.44 Issue 2, pp.461-471

Belge Tipi:

Makale

Yayın Tarihi:

2/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000725449

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

DOI:

https://doi.org/10.3906/kim-1909-53

Özet:

 

The aim of this research was to design an electrochemical immunosensor for determination of tumour necrosis factor receptor-associated protein-1 TRAP1  antigen, a heat shock protein linked to tumour necrosis factor. The indium-tin oxide covered polyethylene terephthalate  ITO-PET  electrode surface was cleaned and was prepared for the introduction of hydroxyl groups on its surface by using NH44_{4} OH/H22_{2}O22_{2}/H22_{2}O. As a silanization agent for covalent attachment of anti-TRAP1 on the surface of the ITO working electrode, 3-glycidoxypropyltrimethoxysilane  3-GOPS  was used. Cyclic voltammetry  CV  and electrochemical impedance spectroscopy  EIS  were used to characterize the immobilization steps. A variety of parameters, 3-GOPS and anti-TRAP1 concentrations, and anti-TRAP1 and TRAP1 incubation durations were optimized. After determining the optimum conditions, characterization studies such asrepeatability,reproducibility,regeneration,squarewavevoltammetry,andsinglefrequencyimpedancewereperformed. The electrochemical immunosensor has presented an extremely wide determination range for TRAP1 from 0.1 pg/mL to 100 pg/mL.

Makale detayları

Development of a biosensor platform based on ITO sheets modified with 3-glycidoxypropyltrimethoxysilane for early detection of TRAP1

Yazarlar: Berfin VURAL | Meltem ÇALIŞKAN | Mustafa Kemal SEZGİNTÜRK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2020 Vol.44 Issue 2, pp.461-471

Belge Tipi: Makale

Yayın Tarihi: 2/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000725449

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

DOI: https://doi.org/10.3906/kim-1909-53

Sayı

Yazarlar:

Berfin VURAL | Meltem ÇALIŞKAN | Mustafa Kemal SEZGİNTÜRK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2020 Vol.44 Issue 2, pp.461-471

Belge Tipi:

Makale

Yayın Tarihi:

2/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000725449

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

DOI:

https://doi.org/10.3906/kim-1909-53