The effect of Lysimachia savranii on the breast canser cells in cell culture conditions compared to normal cells

İşil AYDEMİR; Caner ÖZBEY; Gonca DÖNMEZ ARAT; Oktay ÖZKAN; Ahmet SAVRAN; Sevinç İNAN; Mehmet İbrahim TUĞLU

doi:https://doi.org/10.24998/maeusabed.631012

Makale detayları

The effect of Lysimachia savranii on the breast canser cells in cell culture conditions compared to normal cells

Yazarlar:

İşil AYDEMİR | Caner ÖZBEY | Gonca DÖNMEZ ARAT | Oktay ÖZKAN | Ahmet SAVRAN | Sevinç İNAN | Mehmet İbrahim TUĞLU

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Mehmet Akif Ersoy University Journal of Health Sciences Institute 2019 Vol.7 Issue 2, pp.68-75

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000552073

Sıklık:

Üç Yıllık

pISSN:

2148-2837

DOI:

https://doi.org/10.24998/maeusabed.631012

Özet:

 

The
breast cancer has become a threat to women's lives at an increasing rate. The
inadequate treatments of surgical procedures and chemotherapy affect negatively
quality of patient life. Therefore, there has been an increase in demand for
alternative and supportive agents, especially for the use of herbal products.
The purpose of this study is to evaluate the in vitro anti-tumor activity of Lysimachia savranii in breast cancer
cell lines compared to normal adipose tissue derived mesenchymal stem cells. The
adipose tissue derived mesenchymal stem cells as normal cells with MCF-7 and,
MDA-MB-231 breast cancer cells were cultured. IC50 doses of Lysimachia savranii extract for each
cells were determined via MTT assay. The immunocytochemical staining was
performed for the oxidative stress by eNOS, whereas apoptosis were analyzed by
TUNEL method. The data exhibited with H-score and apoptotic index. The plant
extract inhibited the proliferation and survival in the breast cancer cells compared
to the stem cells. Lysimachia savranii
extract caused the increase of eNOS staining for oxidative stress and the TUNEL
labeling for apoptosis. The further molecular
techniques should be used to determine the biologic activity of Lysimachia
savranii extract and its content
should be investigated.

Makale detayları

The effect of Lysimachia savranii on the breast canser cells in cell culture conditions compared to normal cells

Yazarlar: İşil AYDEMİR | Caner ÖZBEY | Gonca DÖNMEZ ARAT | Oktay ÖZKAN | Ahmet SAVRAN | Sevinç İNAN | Mehmet İbrahim TUĞLU

Yayıncı: Burdur Mehmet Akif Ersoy University

Ana Başlıklar: Sağlık teşvikleri

Dergi: Mehmet Akif Ersoy University Journal of Health Sciences Institute 2019 Vol.7 Issue 2, pp.68-75

Belge Tipi: Makale

Yayın Tarihi: 12/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000552073

Sıklık: Üç Yıllık

pISSN: 2148-2837

DOI: https://doi.org/10.24998/maeusabed.631012

Sayı

Yazarlar:

İşil AYDEMİR | Caner ÖZBEY | Gonca DÖNMEZ ARAT | Oktay ÖZKAN | Ahmet SAVRAN | Sevinç İNAN | Mehmet İbrahim TUĞLU

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Mehmet Akif Ersoy University Journal of Health Sciences Institute 2019 Vol.7 Issue 2, pp.68-75

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000552073

Sıklık:

Üç Yıllık

pISSN:

2148-2837

DOI:

https://doi.org/10.24998/maeusabed.631012