Isolation and Classification of Lipoxygenase from the Rind of Pistacio Khinjuke and Investigation of its Affinity Toward Certain Inhibitors in Mice: in vivo and in vitro

Ameera A. AL-JURAISI; Omar Y. AL-ABBASY

Makale detayları

Isolation and Classification of Lipoxygenase from the Rind of Pistacio Khinjuke and Investigation of its Affinity Toward Certain Inhibitors in Mice: in vivo and in vitro

Yazarlar:

Ameera A. AL-JURAISI | Omar Y. AL-ABBASY

Dergi:

The Eurasia Proceedings of Science Technology Engineering and Mathematics 2018 Issue 3, pp.72-84

Belge Tipi:

Makale

Yayın Tarihi:

11/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000661024

Özet:

 


This study includes purification of
lipoxygenase from the rind of  pistacio
khinjuke by applying ammonium sulfate precipitation (70%), dialysis, ion
exchange chromatography, and the slab electrophoresis technique. One isoenzyme
has been obtained with a special activity of 1230x10-4 unit/mg protein and a molecular
weight of 101997 dalton. The partially purified rind lipoxygenase has been
characterized and inhibited by quercitine rutinoside and melatonine. The type
of inhibition seemed to be uncompetitive and the Ki values were 1.77 and 1.62
mM, respectively. Due to the importance of lipoxygenase for the animal, this
study also deals with the determination of its affinity toward quercitine
rutinoside and melatonine in the serum of normal and diabetic male mice. The
best doses for lipoxygenase inhibition were 523.3 and 8 Mg/Kg body weight of
quercitine rutinoside and melatonine, respectively. Treatment of animals with
these doses led to a significant decrease in lipoxygenase activity, glucose
level, and total cholesterol/high density lipoprotein cholesterol ratio in
animal serum. Therefore, these natural inhibitors seem to serve as a drug for
reducing the complications of some diseases (e.g. diabetes mellitus) which are
affected by this enzyme.

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Makale detayları

Isolation and Classification of Lipoxygenase from the Rind of Pistacio Khinjuke and Investigation of its Affinity Toward Certain Inhibitors in Mice: in vivo and in vitro

Yazarlar: Ameera A. AL-JURAISI | Omar Y. AL-ABBASY

Dergi: The Eurasia Proceedings of Science Technology Engineering and Mathematics 2018 Issue 3, pp.72-84

Belge Tipi: Makale

Yayın Tarihi: 11/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000661024

Sayı

Yazarlar:

Ameera A. AL-JURAISI | Omar Y. AL-ABBASY

Dergi:

The Eurasia Proceedings of Science Technology Engineering and Mathematics 2018 Issue 3, pp.72-84

Belge Tipi:

Makale

Yayın Tarihi:

11/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000661024