Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116

Ana OBRADOVIC; Jovana ZIZIC; Nemanja TRISOVIC; Bojan BOZIC; Gordana USCUMLIC; Biljana BOZIC; Snezana MARKOVIC

Makale detayları

Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116

Yazarlar:

Ana OBRADOVIC | Jovana ZIZIC | Nemanja TRISOVIC | Bojan BOZIC | Gordana USCUMLIC | Biljana BOZIC | Snezana MARKOVIC

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.741-747

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812342

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Antioxidant effects of twelve 3-substituted-5,5-diphenylhydantoins in concentrations of 0.01, 0.1, 1, 10, and 100 µM on human colon cancer cell line HCT-116 were determined 24 h after treatment. The concentrations of superoxide anion radical (O2.-), nitrites (NO2-), and total glutathione were determined spectrophotometrically. Results indicated that treatment with all compounds induced a decrease in O2.- and an increase in NO2- concentrations in HCT-116 cells. Treatment with all compounds resulted in an increase in glutathione, and this indicates changes in redox homeostasis. Based on the obtained results, the investigated hydantoins act as antioxidants because they decrease the production of superoxide anion radical and increase concentrations of glutathione, but they also induce an increase in nitrite production.

 

Antioxidant effects of twelve 3-substituted-5,5-diphenylhydantoins in concentrations of 0.01, 0.1, 1, 10, and 100 µM on human colon cancer cell line HCT-116 were determined 24 h after treatment. The concentrations of superoxide anion radical (O2.-), nitrites (NO2-), and total glutathione were determined spectrophotometrically. Results indicated that treatment with all compounds induced a decrease in O2.- and an increase in NO2- concentrations in HCT-116 cells. Treatment with all compounds resulted in an increase in glutathione, and this indicates changes in redox homeostasis. Based on the obtained results, the investigated hydantoins act as antioxidants because they decrease the production of superoxide anion radical and increase concentrations of glutathione, but they also induce an increase in nitrite production.

Makale detayları

Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116

Yazarlar:

Ana OBRADOVIC | Jovana ZIZIC | Nemanja TRISOVIC | Bojan BOZIC | Gordana USCUMLIC | Biljana BOZIC | Snezana MARKOVIC

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.741-747

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812342

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Antioxidants

phenytoin derivatives

free radicals

redox homeostasis

Sayı

Makale detayları

Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116

Yazarlar: Ana OBRADOVIC | Jovana ZIZIC | Nemanja TRISOVIC | Bojan BOZIC | Gordana USCUMLIC | Biljana BOZIC | Snezana MARKOVIC

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.741-747

Belge Tipi: Makale

Yayın Tarihi: 5/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000812342

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Antioxidants

phenytoin derivatives

free radicals

redox homeostasis

Sayı

Yazarlar:

Ana OBRADOVIC | Jovana ZIZIC | Nemanja TRISOVIC | Bojan BOZIC | Gordana USCUMLIC | Biljana BOZIC | Snezana MARKOVIC

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.741-747

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812342

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152