Isolation and characterization of chemical constituents from the mushroom Clitocybe nebularis

N.A.

Makale detayları

Isolation and characterization of chemical constituents from the mushroom Clitocybe nebularis

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Botany

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 6, pp.908-913

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348015

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 T: In the course of our mycochemical studies the extract of Clitocybe nebularis was investigated with the aim to identify its bioactive secondary metabolites. Multistep chromatographic purification of the MeOH extract of C. nebularis resulted in the isolation of two steroids and an organic acid from the CHCl3 and ethyl acetate soluble fractions. The structures of the compounds were determined by NMR and MS spectroscopy as 5α-ergosta-7,22-diene-3β,5,6β-triol cerevisterol 1, 22E,24S-5α-ergosta-7,22-diene-3β,5,6β,9α-tetraol 2, and indole-3-carboxylic acid 3. The antimicrobial activity of the compounds was analyzed by agar disc diffusion method against human pathogen strains of Streptococcus agalactiae, Staphylococcus epidermidis, Moraxella catarrhalis, Haemophilus influenzae, and Proteus mirabilis. The susceptibility assay revealed that compounds 2 and 3 have weak antimicrobial activity against M. catarrhalis. The current study represents the first isolation of compounds 13 from C. nebularis.

 T: In the course of our mycochemical studies the extract of Clitocybe nebularis was investigated with the aim to identify its bioactive secondary metabolites. Multistep chromatographic purification of the MeOH extract of C. nebularis resulted in the isolation of two steroids and an organic acid from the CHCl3 and ethyl acetate soluble fractions. The structures of the compounds were determined by NMR and MS spectroscopy as 5α-ergosta-7,22-diene-3β,5,6β-triol cerevisterol 1, 22E,24S-5α-ergosta-7,22-diene-3β,5,6β,9α-tetraol 2, and indole-3-carboxylic acid 3. The antimicrobial activity of the compounds was analyzed by agar disc diffusion method against human pathogen strains of Streptococcus agalactiae, Staphylococcus epidermidis, Moraxella catarrhalis, Haemophilus influenzae, and Proteus mirabilis. The susceptibility assay revealed that compounds 2 and 3 have weak antimicrobial activity against M. catarrhalis. The current study represents the first isolation of compounds 13 from C. nebularis.

 T: In the course of our mycochemical studies the extract of Clitocybe nebularis was investigated with the aim to identify its bioactive secondary metabolites. Multistep chromatographic purification of the MeOH extract of C. nebularis resulted in the isolation of two steroids and an organic acid from the CHCl3 and ethyl acetate soluble fractions. The structures of the compounds were determined by NMR and MS spectroscopy as 5α-ergosta-7,22-diene-3β,5,6β-triol cerevisterol 1, 22E,24S-5α-ergosta-7,22-diene-3β,5,6β,9α-tetraol 2, and indole-3-carboxylic acid 3. The antimicrobial activity of the compounds was analyzed by agar disc diffusion method against human pathogen strains of Streptococcus agalactiae, Staphylococcus epidermidis, Moraxella catarrhalis, Haemophilus influenzae, and Proteus mirabilis. The susceptibility assay revealed that compounds 2 and 3 have weak antimicrobial activity against M. catarrhalis. The current study represents the first isolation of compounds 13 from C. nebularis.

 T: In the course of our mycochemical studies the extract of Clitocybe nebularis was investigated with the aim to identify its bioactive secondary metabolites. Multistep chromatographic purification of the MeOH extract of C. nebularis resulted in the isolation of two steroids and an organic acid from the CHCl3 and ethyl acetate soluble fractions. The structures of the compounds were determined by NMR and MS spectroscopy as 5α-ergosta-7,22-diene-3β,5,6β-triol cerevisterol 1, 22E,24S-5α-ergosta-7,22-diene-3β,5,6β,9α-tetraol 2, and indole-3-carboxylic acid 3. The antimicrobial activity of the compounds was analyzed by agar disc diffusion method against human pathogen strains of Streptococcus agalactiae, Staphylococcus epidermidis, Moraxella catarrhalis, Haemophilus influenzae, and Proteus mirabilis. The susceptibility assay revealed that compounds 2 and 3 have weak antimicrobial activity against M. catarrhalis. The current study represents the first isolation of compounds 13 from C. nebularis.

Makale detayları

Isolation and characterization of chemical constituents from the mushroom Clitocybe nebularis

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Botany

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 6, pp.908-913

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348015

pISSN:

2630-6344

Clitocybe nebularis

steroids

antimicrobial activity

Tricholomataceae

Sayı

Makale detayları

Isolation and characterization of chemical constituents from the mushroom Clitocybe nebularis

Yazarlar: N.A.

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Botany

Dergi: Journal of Research in Pharmacy Vol.24 Issue 6, pp.908-913

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001348015

pISSN: 2630-6344

Clitocybe nebularis

steroids

antimicrobial activity

Tricholomataceae

Sayı

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Botany

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 6, pp.908-913

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348015

pISSN:

2630-6344