In vitro Plant Regeneration from callus in semi dwarf cultivar of Punica granatum L. ʻNanaʼ

Alireza BONYANPOUR; Morteza KHOSH-KHUI

Makale detayları

In vitro Plant Regeneration from callus in semi dwarf cultivar of Punica granatum L. ʻNanaʼ

Yazarlar:

Alireza BONYANPOUR | Morteza KHOSH-KHUI

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2013 Vol.7 Issue 1, pp.47-51

Belge Tipi:

Makale

Yayın Tarihi:

1/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000831457

Sıklık:

Üç Yıllık

eISSN:

2146-0108

Özet:

 

Leaf explants of a semi dwarf cultivar of pomegranate were placed on Murashige and Skoog [6] (MS) medium supplemented with various concentrations of 6-benzyl adenin (BA) and naphthalene acetic acid (NAA) for callus induction. Maximum callus induction was observed on a media containing 1 mg L-1 BA with 0 mg L-1 NAA. However, the highest callus growth was obtained on a medium containing 1 mg L-1 BA and 0.5 mg L-1 NAA.. Maximum shoot proliferation was observed when shoots were cultured on woody plant medium (WPM) supplemented with 2 mg L-1 BA. In this treatment, after 4 subcultures, 85 shoots were produced from one original explant. Among treatments used in rooting experiments, shoots cultured on WPM medium with 0-0.2 mg L-1 indol butyric acid (IBA) had the maximum root percentage (100%). Rooted plantlets were cultured in a soil mixture containing vermiculite (60%), perlite (30%) and coco peat (10%) v/v. After 2 months, 100% of plants survived and transferred to the greenhouse.

Makale detayları

In vitro Plant Regeneration from callus in semi dwarf cultivar of Punica granatum L. ʻNanaʼ

Yazarlar: Alireza BONYANPOUR | Morteza KHOSH-KHUI

Yayıncı: Nobel Bilim ve Araştırma Merkezi Limited

Dergi: Journal of Applied Biological Sciences 2013 Vol.7 Issue 1, pp.47-51

Belge Tipi: Makale

Yayın Tarihi: 1/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000831457

Sıklık: Üç Yıllık

eISSN: 2146-0108

Sayı

Yazarlar:

Alireza BONYANPOUR | Morteza KHOSH-KHUI

Yayıncı:

Nobel Bilim ve Araştırma Merkezi Limited

Dergi:

Journal of Applied Biological Sciences 2013 Vol.7 Issue 1, pp.47-51

Belge Tipi:

Makale

Yayın Tarihi:

1/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000831457

Sıklık:

Üç Yıllık

eISSN:

2146-0108