Investigation of the Effects of Ghrelin and Kisspeptin Levels in Liver Tissue of Rats Fed with High Fructose Diet -A Histological Study

Ahmet Türk; Abdullah Karadağ; Büşra Zencirci; Yusuf Özay; Osman Güler

Makale detayları

Investigation of the Effects of Ghrelin and Kisspeptin Levels in Liver Tissue of Rats Fed with High Fructose Diet -A Histological Study

Yazarlar:

Ahmet Türk | Abdullah Karadağ | Büşra Zencirci | Yusuf Özay | Osman Güler

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Ahi Evran Tıp Dergisi Vol.7 Issue 3, pp.346-352

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001300280

pISSN:

2619-9203

Anahtar kelimeler:

Özet:

 Purpose: High fructose corn syrup HFCS is used commonly in the food industry and has been associated with various diseases including obesity, diabetes and fatty liver. Ghrelin, an orexigenic hormone with widespread receptors in many tissues, exerts various central and peripheral effects. Food intake may reduce its synthesis. Kisspeptin plays a major role in reproductive hormones and its receptors are expressed in tissues with high metabolic activity, such as the liver. This study aims to investigate the effects of HFCS consumption on liver tissue and its relationship with Kisspeptin and Ghrelin levels. Materials and Methods: Fourteen adult male Wistar albino rats, aged eight to ten weeks, were used in this study and divided into two groups; Control and HFCS n7. Tumor Necrosis Factor-alpha TNF-α, Ghrelin, and Kisspeptin levels in the liver tissues of the rats were measured using an immunohistochemical method and subsequently analyzed through histoscoring. Results: Our data revealed a significant increase in Kisspeptin, Caspase 3, and TNF-α levels and a decrease in Ghrelin levels in the HFCS group compared to the Control group. Conclusion: In conclusion, our study demonstrates, for the first time, changes in liver Kisspeptin levels induced by a high fructose diet. Furthermore, the consumption of HFCS in food led to inflammatory activation, tissue damage, and reduced Ghrelin levels.

 Purpose: High fructose corn syrup HFCS is used commonly in the food industry and has been associated with various diseases including obesity, diabetes and fatty liver. Ghrelin, an orexigenic hormone with widespread receptors in many tissues, exerts various central and peripheral effects. Food intake may reduce its synthesis. Kisspeptin plays a major role in reproductive hormones and its receptors are expressed in tissues with high metabolic activity, such as the liver. This study aims to investigate the effects of HFCS consumption on liver tissue and its relationship with Kisspeptin and Ghrelin levels. Materials and Methods: Fourteen adult male Wistar albino rats, aged eight to ten weeks, were used in this study and divided into two groups; Control and HFCS n7. Tumor Necrosis Factor-alpha TNF-α, Ghrelin, and Kisspeptin levels in the liver tissues of the rats were measured using an immunohistochemical method and subsequently analyzed through histoscoring. Results: Our data revealed a significant increase in Kisspeptin, Caspase 3, and TNF-α levels and a decrease in Ghrelin levels in the HFCS group compared to the Control group. Conclusion: In conclusion, our study demonstrates, for the first time, changes in liver Kisspeptin levels induced by a high fructose diet. Furthermore, the consumption of HFCS in food led to inflammatory activation, tissue damage, and reduced Ghrelin levels.

 Purpose: High fructose corn syrup HFCS is used commonly in the food industry and has been associated with various diseases including obesity, diabetes and fatty liver. Ghrelin, an orexigenic hormone with widespread receptors in many tissues, exerts various central and peripheral effects. Food intake may reduce its synthesis. Kisspeptin plays a major role in reproductive hormones and its receptors are expressed in tissues with high metabolic activity, such as the liver. This study aims to investigate the effects of HFCS consumption on liver tissue and its relationship with Kisspeptin and Ghrelin levels. Materials and Methods: Fourteen adult male Wistar albino rats, aged eight to ten weeks, were used in this study and divided into two groups; Control and HFCS n7. Tumor Necrosis Factor-alpha TNF-α, Ghrelin, and Kisspeptin levels in the liver tissues of the rats were measured using an immunohistochemical method and subsequently analyzed through histoscoring. Results: Our data revealed a significant increase in Kisspeptin, Caspase 3, and TNF-α levels and a decrease in Ghrelin levels in the HFCS group compared to the Control group. Conclusion: In conclusion, our study demonstrates, for the first time, changes in liver Kisspeptin levels induced by a high fructose diet. Furthermore, the consumption of HFCS in food led to inflammatory activation, tissue damage, and reduced Ghrelin levels.

 Amaç: Yüksek fruktozlu mısır şurubuYFMŞ, gıda endüstrisinde yaygın olarak kullanılmakta olup; obezite, diyabet ve yağlı karaciğer hastalığı gibi birçok hastalıkla ilişkilidir. Ghrelin, merkezi ve periferik etkilere sahip birçok dokuda yaygın reseptörlere sahip olmasının yanı sıra oreksijenik bir hormondur. Gıda alınımını azaltabilmektedir. Kisspeptin, üreme hormonlarında önemli bir rol oynamakta ve karaciğer dâhil olmak üzere yüksek metabolik aktiviteye sahip birçok dokuda reseptörü bulunmaktadır. Bu çalışmada YFMŞ tüketiminin karaciğer dokusu üzerindeki etkilerinin ve bunun Kisspeptin ve Ghrelin seviyeleri ile ilişkisinin incelenmesi amaçlanmaktadır. Araçlar ve Yöntem: Çalışmada 8-10 haftalık, 14 adet erişkin Wistar albino erkek sıçan kullanıldı ve 2 gruba ayrıldı Kontrol, YFMŞ n7. Sıçanların karaciğer dokularındaki Kaspaz 3, TNF-α, Ghrelin ve Kisspeptin seviyeleri immünohistokimyasal yöntemle ölçüldü ve ardından histoskorlama ile analiz edildi. Bulgular: Verilerimize göre YFMŞ grubunda kontrol grubuna göre Kisspeptin, Kaspase 3, TNF-α düzeylerinde anlamlı artış ve Ghrelin düzeylerinde azalma gözlemlendi. Sonuç: Sonuç olarak, çalışmamızda yüksek fruktozlu diyetin karaciğer Kisspeptin düzeylerinde neden olduğu değişiklikler ilk kez gösterilmiştir. Ayrıca, gıdada YFMŞ kullanımı inflamatuar aktivasyona, doku hasarına ve Ghrelin düzeylerinin düşmesine neden olmuştur.

Makale detayları

Investigation of the Effects of Ghrelin and Kisspeptin Levels in Liver Tissue of Rats Fed with High Fructose Diet -A Histological Study

Yazarlar:

Ahmet Türk | Abdullah Karadağ | Büşra Zencirci | Yusuf Özay | Osman Güler

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Ahi Evran Tıp Dergisi Vol.7 Issue 3, pp.346-352

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001300280

pISSN:

2619-9203

Apoptosis

ghrelin

kisspeptin

proinflammatory cytokinesapoptozis

ghrelin

kisspeptin

proinflamatuvar sitokinler

Sayı

Makale detayları

Yazarlar: Ahmet Türk | Abdullah Karadağ | Büşra Zencirci | Yusuf Özay | Osman Güler

Yayıncı: Kirsehir Ahi Evran University

Ana Başlıklar: Klinik Tıp Bilimleri

Dergi: Ahi Evran Tıp Dergisi Vol.7 Issue 3, pp.346-352

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001300280

pISSN: 2619-9203

Sayı

Yazarlar:

Ahmet Türk | Abdullah Karadağ | Büşra Zencirci | Yusuf Özay | Osman Güler

Yayıncı:

Kirsehir Ahi Evran University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Ahi Evran Tıp Dergisi Vol.7 Issue 3, pp.346-352

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001300280

pISSN:

2619-9203