Identification of watermelon heat shock protein members and tissue-specific gene expression analysis under combined drought and heat stresses

Yasemin Çelik ALTUNOĞLU; Merve KELEŞ; Tevfik Hasan CAN; Mehmet Cengiz BALOĞLU

Makale detayları

Identification of watermelon heat shock protein members and tissue-specific gene expression analysis under combined drought and heat stresses

Yazarlar:

Yasemin Çelik ALTUNOĞLU | Merve KELEŞ | Tevfik Hasan CAN | Mehmet Cengiz BALOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2019 Vol.43 Issue 6, pp.404-419

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000811059

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Heat shock protein (Hsp) gene family members in the watermelon genome were identified and characterized by bioinformatics  analysis. In addition, expression profiles of genes under combined drought and heat stress conditions were experimentally analyzed.  In the watermelon genome, 39 genes belonging to the sHsp family, 101 genes belonging to the Hsp40 family, 23 genes belonging to the  Hsp60 family, 12 genes belonging to the Hsp70 family, 6 genes belonging to the Hsp90 family, and 102 genes belonging to the Hsp100  family were found. It was also observed that the proteins in the same cluster in the phylogenetic trees had similar motif patterns. When  the estimated 3-dimensional structures of the Hsp proteins were examined, it was determined that the ?-helical structure was dominant  in almost all families. The most orthologous relationship appeared to be between watermelon, soybean, and poplar in the ClaHsp gene  families. For tissue-specific gene expression analysis under combined stress conditions, expression analysis of one representative Hsp  gene each from root, stem, leaf, and shoot tissues was performed by real-time PCR. A significant increase was detected usually at 30  min in almost all tissues. This study provides extensive information for watermelon Hsps, and can enhance our knowledge about the  relationships between Hsp genes and combined stresses.

Makale detayları

Identification of watermelon heat shock protein members and tissue-specific gene expression analysis under combined drought and heat stresses

Yazarlar: Yasemin Çelik ALTUNOĞLU | Merve KELEŞ | Tevfik Hasan CAN | Mehmet Cengiz BALOĞLU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2019 Vol.43 Issue 6, pp.404-419

Belge Tipi: Makale

Yayın Tarihi: 11/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000811059

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Yasemin Çelik ALTUNOĞLU | Merve KELEŞ | Tevfik Hasan CAN | Mehmet Cengiz BALOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2019 Vol.43 Issue 6, pp.404-419

Belge Tipi:

Makale

Yayın Tarihi:

11/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000811059

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152