Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea

Kutsal KESİCİ; İnci TÜNEY; Doğuş ZEREN; Mustafa GÜDEN; Atakan SUKATAR

doi:https://doi.org/10.3906/biy-1205-40

Makale detayları

Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea

Yazarlar:

Kutsal KESİCİ | İnci TÜNEY | Doğuş ZEREN | Mustafa GÜDEN | Atakan SUKATAR

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.530-537

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000812352

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1205-40

Anahtar kelimeler:

Özet:

 

Diatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but they also play a key role in the biogeochemical cycling of silica. Molecular identification methods based on conserved DNA sequences, such as internal transcribed spacer (ITS) have revolutionized our knowledge and understanding of conventional taxonomy. In this study, we aimed to compare the conventional identification methods with molecular identification methods. To do so, we isolated 4 diatom samples from the coast at Urla and characterized them using light microscopy (LM) and scanning electron microscopy (SEM) according to morphological features. Then we amplified ITS regions using a conventional polymerase chain reaction (PCR), sequenced the PCR products, and analyzed the sequences using bioinformatic tools. Bioinformatic analysis indicated that the isolated species had high sequence similarity to Pseudo-nitzschia delicatissima, Achnanthes taeniata, Amphora coffeaeformis, and Cylindrotheca closterium. We think that molecular identification methods enable rapid and more reliable identification of diatom species and are crucial for monitoring harmful algal blooms.

 

Diatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but they also play a key role in the biogeochemical cycling of silica. Molecular identification methods based on conserved DNA sequences, such as internal transcribed spacer (ITS) have revolutionized our knowledge and understanding of conventional taxonomy. In this study, we aimed to compare the conventional identification methods with molecular identification methods. To do so, we isolated 4 diatom samples from the coast at Urla and characterized them using light microscopy (LM) and scanning electron microscopy (SEM) according to morphological features. Then we amplified ITS regions using a conventional polymerase chain reaction (PCR), sequenced the PCR products, and analyzed the sequences using bioinformatic tools. Bioinformatic analysis indicated that the isolated species had high sequence similarity to Pseudo-nitzschia delicatissima, Achnanthes taeniata, Amphora coffeaeformis, and Cylindrotheca closterium. We think that molecular identification methods enable rapid and more reliable identification of diatom species and are crucial for monitoring harmful algal blooms.

Makale detayları

Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea

Yazarlar:

Kutsal KESİCİ | İnci TÜNEY | Doğuş ZEREN | Mustafa GÜDEN | Atakan SUKATAR

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.530-537

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000812352

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1205-40

Key words: Diatom

ITS

LM

SEM

molecular characterization

Sayı

Makale detayları

Morphological and molecular identification of pennate diatoms isolated from Urla, İzmir, coast of the Aegean Sea

Yazarlar: Kutsal KESİCİ | İnci TÜNEY | Doğuş ZEREN | Mustafa GÜDEN | Atakan SUKATAR

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.530-537

Belge Tipi: Makale

Yayın Tarihi: 4/2013

Belge Dili: İngilizce

Document ID (Org No.): 2000812352

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

DOI: https://doi.org/10.3906/biy-1205-40

Key words: Diatom

ITS

LM

SEM

molecular characterization

Sayı

Yazarlar:

Kutsal KESİCİ | İnci TÜNEY | Doğuş ZEREN | Mustafa GÜDEN | Atakan SUKATAR

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.530-537

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000812352

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1205-40