Boscalid + Pyraclotrobin Resistance and Expression of Succinate Dehyrogenase Genes (sdhA and sdhB) in Chestnut Blight Fungus Cryphonectria parasitica

Ebru Derelli Tüfekçi; Deniz Çakar; Seçil Akıllı Şimşek

Makale detayları

Boscalid + Pyraclotrobin Resistance and Expression of Succinate Dehyrogenase Genes (sdhA and sdhB) in Chestnut Blight Fungus Cryphonectria parasitica

Yazarlar:

Ebru Derelli Tüfekçi | Deniz Çakar | Seçil Akıllı Şimşek

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Gen İfadesi

Dergi:

Black Sea Journal of Agriculture Vol.7 Issue 3, pp.287-297

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314945

pISSN:

2618-6578

Anahtar kelimeler:

Özet:

 Sweet chestnut, Castanea sativa Mill., is a significant species in Türkiye. The tree has been encountered with numerous diseases. Chestnut blight, caused by Cryphonectria parasitica Murrill M. E. Barr, is one of the most common diseases having spread to every chestnut-growing region. In this study, the effects of Bellis 25.2 Boscalid  12.8 Pyraclotrobin, a fungicide commonly used in plant disease control, were evaluated at different doses 10 ppm and 50 ppm against the chestnut blight agent C. parasitica, which had not been previously studied for its effects in vitro. The isolates included two virulent strains Cp1_2023, Cp2_2023 and two hypovirulent strains Cp7_2023, Cp9_2023. The effects of Boscalid  Pyraclotrobin on the mycelial growth of the pathogenic fungus were determined for the first time in vitro, and these effects were detected at the sdhA and sdhB genes expression level. The results showed that the mycelial growth and pathogenicity of C. parasitica isolates in the fungicide-treated medium were consistent with the gene expression profile. Specifically, the virulent Cp1_2023 isolate exhibited higher mycelial growth, pathogenicity, and gene expression levels compared to the other isolate. In conclusion, it is recommended to conduct more detailed studies before using fungicides and to employ them in controlled environments.

 Sweet chestnut, Castanea sativa Mill., is a significant species in Türkiye. The tree has been encountered with numerous diseases. Chestnut blight, caused by Cryphonectria parasitica Murrill M. E. Barr, is one of the most common diseases having spread to every chestnut-growing region. In this study, the effects of Bellis 25.2 Boscalid  12.8 Pyraclotrobin, a fungicide commonly used in plant disease control, were evaluated at different doses 10 ppm and 50 ppm against the chestnut blight agent C. parasitica, which had not been previously studied for its effects in vitro. The isolates included two virulent strains Cp1_2023, Cp2_2023 and two hypovirulent strains Cp7_2023, Cp9_2023. The effects of Boscalid  Pyraclotrobin on the mycelial growth of the pathogenic fungus were determined for the first time in vitro, and these effects were detected at the sdhA and sdhB genes expression level. The results showed that the mycelial growth and pathogenicity of C. parasitica isolates in the fungicide-treated medium were consistent with the gene expression profile. Specifically, the virulent Cp1_2023 isolate exhibited higher mycelial growth, pathogenicity, and gene expression levels compared to the other isolate. In conclusion, it is recommended to conduct more detailed studies before using fungicides and to employ them in controlled environments.

 Sweet chestnut, Castanea sativa Mill., is a significant species in Türkiye. The tree has been encountered with numerous diseases. Chestnut blight, caused by Cryphonectria parasitica Murrill M. E. Barr, is one of the most common diseases having spread to every chestnut-growing region. In this study, the effects of Bellis 25.2 Boscalid  12.8 Pyraclotrobin, a fungicide commonly used in plant disease control, were evaluated at different doses 10 ppm and 50 ppm against the chestnut blight agent C. parasitica, which had not been previously studied for its effects in vitro. The isolates included two virulent strains Cp1_2023, Cp2_2023 and two hypovirulent strains Cp7_2023, Cp9_2023. The effects of Boscalid  Pyraclotrobin on the mycelial growth of the pathogenic fungus were determined for the first time in vitro, and these effects were detected at the sdhA and sdhB genes expression level. The results showed that the mycelial growth and pathogenicity of C. parasitica isolates in the fungicide-treated medium were consistent with the gene expression profile. Specifically, the virulent Cp1_2023 isolate exhibited higher mycelial growth, pathogenicity, and gene expression levels compared to the other isolate. In conclusion, it is recommended to conduct more detailed studies before using fungicides and to employ them in controlled environments.

 Sweet chestnut, Castanea sativa Mill., is a significant species in Türkiye. The tree has been encountered with numerous diseases. Chestnut blight, caused by Cryphonectria parasitica Murrill M. E. Barr, is one of the most common diseases having spread to every chestnut-growing region. In this study, the effects of Bellis 25.2 Boscalid  12.8 Pyraclotrobin, a fungicide commonly used in plant disease control, were evaluated at different doses 10 ppm and 50 ppm against the chestnut blight agent C. parasitica, which had not been previously studied for its effects in vitro. The isolates included two virulent strains Cp1_2023, Cp2_2023 and two hypovirulent strains Cp7_2023, Cp9_2023. The effects of Boscalid  Pyraclotrobin on the mycelial growth of the pathogenic fungus were determined for the first time in vitro, and these effects were detected at the sdhA and sdhB genes expression level. The results showed that the mycelial growth and pathogenicity of C. parasitica isolates in the fungicide-treated medium were consistent with the gene expression profile. Specifically, the virulent Cp1_2023 isolate exhibited higher mycelial growth, pathogenicity, and gene expression levels compared to the other isolate. In conclusion, it is recommended to conduct more detailed studies before using fungicides and to employ them in controlled environments.

Makale detayları

Boscalid + Pyraclotrobin Resistance and Expression of Succinate Dehyrogenase Genes (sdhA and sdhB) in Chestnut Blight Fungus Cryphonectria parasitica

Yazarlar:

Ebru Derelli Tüfekçi | Deniz Çakar | Seçil Akıllı Şimşek

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Gen İfadesi

Dergi:

Black Sea Journal of Agriculture Vol.7 Issue 3, pp.287-297

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314945

pISSN:

2618-6578

Chestnut

Cryphonectria parasitica

Fungicide

Sdh genes

Sayı

Makale detayları

Boscalid + Pyraclotrobin Resistance and Expression of Succinate Dehyrogenase Genes (sdhA and sdhB) in Chestnut Blight Fungus Cryphonectria parasitica

Yazarlar: Ebru Derelli Tüfekçi | Deniz Çakar | Seçil Akıllı Şimşek

Yayıncı: Karyay Karadeniz Yayımcılık

Ana Başlıklar: Gen İfadesi

Dergi: Black Sea Journal of Agriculture Vol.7 Issue 3, pp.287-297

Belge Tipi: Makale

Yayın Tarihi: 5/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001314945

pISSN: 2618-6578

Chestnut

Cryphonectria parasitica

Fungicide

Sdh genes

Sayı

Yazarlar:

Ebru Derelli Tüfekçi | Deniz Çakar | Seçil Akıllı Şimşek

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Gen İfadesi

Dergi:

Black Sea Journal of Agriculture Vol.7 Issue 3, pp.287-297

Belge Tipi:

Makale

Yayın Tarihi:

5/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001314945

pISSN:

2618-6578