Evaluation of diagnostic methods for the detection of pestiviruses in clinical samples

Pelin TUNCER GÖKTUNA; Kadir YEŞİLBAĞ

Makale detayları

Evaluation of diagnostic methods for the detection of pestiviruses in clinical samples

Yazarlar:

Pelin TUNCER GÖKTUNA | Kadir YEŞİLBAĞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2017 Vol.41 Issue 2, pp.175-179

Belge Tipi:

Makale

Yayın Tarihi:

4/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000765011

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Özet:

 

There are several commonly used diagnostic methods to detect pestiviruses for routine diagnostic purposes. In the present
study, we aimed to compare virus isolation-indirect immunoperoxidase monolayer assay (VI-IIPMA), antigen capture ELISA (ACE),
and RT-PCR for the detection of pestiviruses in clinical samples. Out of 246 samples tested (11 serum, 119 swab, 116 tissue), 28 samples
(11.39%) were positive and 218 (88.61%) were negative using the VI-IIPMA method. Using ACE, 70 samples (28.46%) were positive
and 176 (71.54%) were negative. Finally, using RT-PCR analysis, we detected 19 (7.72%) positive and 227 (92.28%) negative samples.
Inconsistencies were observed among results of the three methods: 8 samples were positive using VI-IIPMA but negative using ACE
and RT-PCR. In addition, 4 samples that were found to be negative by VI-IIPMA were found to be positive by ACE and RT-PCR. Five
samples were positive by ACE and VI-IIPMA. However, 46 samples were found to be positive only by ACE. These results show that the
number of positive results detected by ACE is higher than that by VI-IIPMA and RT-PCR. Although ACE may prove advantageous for
diagnosing pestiviruses, using a second method in combination with ACE will improve the validity of the results.

Makale detayları

Evaluation of diagnostic methods for the detection of pestiviruses in clinical samples

Yazarlar: Pelin TUNCER GÖKTUNA | Kadir YEŞİLBAĞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2017 Vol.41 Issue 2, pp.175-179

Belge Tipi: Makale

Yayın Tarihi: 4/2017

Belge Dili: İngilizce

Document ID (Org No.): 2000765011

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

Pelin TUNCER GÖKTUNA | Kadir YEŞİLBAĞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2017 Vol.41 Issue 2, pp.175-179

Belge Tipi:

Makale

Yayın Tarihi:

4/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000765011

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128