Investigation of PLGF, sFlt-1 Expression in Placenta Previa, Placenta Accreta and Normotensive Placentas in the 3rd Trimester of Pregnancy

Gamze Erdoğan; Seval Kaya; Nurullah Peker; İbrahim İbiloğlu; Engin Deveci

Makale detayları

Investigation of PLGF, sFlt-1 Expression in Placenta Previa, Placenta Accreta and Normotensive Placentas in the 3rd Trimester of Pregnancy

Yazarlar:

Gamze Erdoğan | Seval Kaya | Nurullah Peker | İbrahim İbiloğlu | Engin Deveci

Yayıncı:

Harran University

Ana Başlıklar:

Histoloji ve Embriyoloji

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.21 Issue 3, pp.407-414

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327466

pISSN:

1304-9623

Anahtar kelimeler:

Özet:

 Background: We aimed to immunohistochemically examine the expression level of Placental growth factor PLGF and Soluble FMS-like tyrosine kinase-1 sFlt-1 proteins in normotensive, Placenta accreta PA and Placenta previa PP. Materials and Methods: Three groups were created for the study: 20 Nomotensive placentas, 20 PP and 20 PA from women diagnosed with placenta samples. 4-6 μm thick sections were taken from the placentas, PLGF and sFlt-1 immunostaining were applied to the obtained sections, and their expression intensities and localizations in the tissues were determined. Results: As a result of our observations, normotensive placenta group; sFlt-1 expression was observed in hofbauer cells and syncytial nodes and PLGF positive expression was observed in nuclei of muscle cells in some tunica media region. PP and PA group; sFlt-1 expression was positive in decidua cells, hofbauer cells and dilated vascular endothelium. PP and PA group; negative PLGF expression was observed in syncytial nodes and positive PLGF expression was observed in hofbauer cells and endothelial cells. There was a significant difference between normotensive, PP and PA groups p0.05. Conclusions: It can be thought that sFlt-1 and PLGF may be important signal stimulators and markers in the trophoblastic degeneration, apoptotic cells, increase in angiogenesis and imbalance in implanta-tion seen in cases of PP and PA.

 Background: We aimed to immunohistochemically examine the expression level of Placental growth factor PLGF and Soluble FMS-like tyrosine kinase-1 sFlt-1 proteins in normotensive, Placenta accreta PA and Placenta previa PP. Materials and Methods: Three groups were created for the study: 20 Nomotensive placentas, 20 PP and 20 PA from women diagnosed with placenta samples. 4-6 μm thick sections were taken from the placentas, PLGF and sFlt-1 immunostaining were applied to the obtained sections, and their expression intensities and localizations in the tissues were determined. Results: As a result of our observations, normotensive placenta group; sFlt-1 expression was observed in hofbauer cells and syncytial nodes and PLGF positive expression was observed in nuclei of muscle cells in some tunica media region. PP and PA group; sFlt-1 expression was positive in decidua cells, hofbauer cells and dilated vascular endothelium. PP and PA group; negative PLGF expression was observed in syncytial nodes and positive PLGF expression was observed in hofbauer cells and endothelial cells. There was a significant difference between normotensive, PP and PA groups p0.05. Conclusions: It can be thought that sFlt-1 and PLGF may be important signal stimulators and markers in the trophoblastic degeneration, apoptotic cells, increase in angiogenesis and imbalance in implanta-tion seen in cases of PP and PA.

 Background: We aimed to immunohistochemically examine the expression level of Placental growth factor PLGF and Soluble FMS-like tyrosine kinase-1 sFlt-1 proteins in normotensive, Placenta accreta PA and Placenta previa PP. Materials and Methods: Three groups were created for the study: 20 Nomotensive placentas, 20 PP and 20 PA from women diagnosed with placenta samples. 4-6 μm thick sections were taken from the placentas, PLGF and sFlt-1 immunostaining were applied to the obtained sections, and their expression intensities and localizations in the tissues were determined. Results: As a result of our observations, normotensive placenta group; sFlt-1 expression was observed in hofbauer cells and syncytial nodes and PLGF positive expression was observed in nuclei of muscle cells in some tunica media region. PP and PA group; sFlt-1 expression was positive in decidua cells, hofbauer cells and dilated vascular endothelium. PP and PA group; negative PLGF expression was observed in syncytial nodes and positive PLGF expression was observed in hofbauer cells and endothelial cells. There was a significant difference between normotensive, PP and PA groups p0.05. Conclusions: It can be thought that sFlt-1 and PLGF may be important signal stimulators and markers in the trophoblastic degeneration, apoptotic cells, increase in angiogenesis and imbalance in implanta-tion seen in cases of PP and PA.

 Amaç: Normotensif, Plasenta akreata PA ve Plasenta previa PP; Plasenta büyüme faktör PLGF ve Soluble Fms-Benzeri Tirozin Kinaz-1 sFlt-1 proteinlerin ekspresyon düzeylerinin immünohistokimyasal olarak incelenmesini amaçladık. Materyal ve Metod: Çalışmaya 20 Nomotensif plasenta, 20 PP ve 20 PA tanısı konmuş kadına ait plasen-ta örnekleri olmak üzere 3 grup oluşturulmuştur. Plasentalardan 4-6 μm kalınlığında kesitler alındı, elde edilen kesitlere PLGF ve sFlt-1 immün boyama uygulanarak dokulardaki ekspresyon şiddetleri ve loka-lizasyonları belirlendi. Bulgular: Gözlemlerimiz sonucu normotensif plasenta grubu; hofbauer hücreler ve sinsityal nodlar sFlt-1 ve bazı tunika media bölgesindeki kas hücrelerin nukleuslarında PLGF pozitif ekspresyonu izlendi. PP ve PA grubu; desidua hücreler, hofbauer hücrelerinde ve dilate damar endotelinde sFlt-1 ekspresyonu pozitif izlendi. PP ve PA grubu; sinsityal düğümlerde negatif, hofbauer hücrelerinde ve endotel hücre-lerinde pozitif PLGF ekspresyonu izlendi. Normotensif, PP ve PA grupları arasında anlamlı bir fark vardı p0.05. Sonuç: PP ve PA olgularında görülen trofoblastik dejenerasyon ve apoptotik hücreler, anjiyogenezisin artmasına ve implantasyonda dengesizliğin gerçekleşmesinde sFlt-1 ve PLGF etkili olabileceğini düşün-mekteyiz.

Makale detayları

Investigation of PLGF, sFlt-1 Expression in Placenta Previa, Placenta Accreta and Normotensive Placentas in the 3rd Trimester of Pregnancy

Yazarlar:

Gamze Erdoğan | Seval Kaya | Nurullah Peker | İbrahim İbiloğlu | Engin Deveci

Yayıncı:

Harran University

Ana Başlıklar:

Histoloji ve Embriyoloji

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.21 Issue 3, pp.407-414

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327466

pISSN:

1304-9623

Placenta accreta

Placenta previa

PLGF

sFlt-1Plasenta akreata

Plasenta previa

PLGF

sFlt-1

Sayı

Makale detayları

Yazarlar: Gamze Erdoğan | Seval Kaya | Nurullah Peker | İbrahim İbiloğlu | Engin Deveci

Yayıncı: Harran University

Ana Başlıklar: Histoloji ve Embriyoloji

Dergi: Harran Üniversitesi Tıp Fakültesi Dergisi Vol.21 Issue 3, pp.407-414

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001327466

pISSN: 1304-9623

Sayı

Yazarlar:

Gamze Erdoğan | Seval Kaya | Nurullah Peker | İbrahim İbiloğlu | Engin Deveci

Yayıncı:

Harran University

Ana Başlıklar:

Histoloji ve Embriyoloji

Dergi:

Harran Üniversitesi Tıp Fakültesi Dergisi Vol.21 Issue 3, pp.407-414

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327466

pISSN:

1304-9623