Preconcentrations of Cd(II) and Cu(II) by solid phase extraction on thermophilic Bacillus firmus loaded Amberlite XAD-4 prior to their determinationsin food samples by ICP-OES

Sadin ÖZDEMİR

Makale detayları

Preconcentrations of Cd(II) and Cu(II) by solid phase extraction on thermophilic Bacillus firmus loaded Amberlite XAD-4 prior to their determinationsin food samples by ICP-OES

Yazarlar:

Sadin ÖZDEMİR

Yayıncı:

Van Yuzuncu Yıl University

Dergi:

Yuzuncu Yil University Journal of the Institute of Natural and Applied Sciences 2016 Vol.21 Issue 2, pp.152-166

Belge Tipi:

Makale

Yayın Tarihi:

12/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000757001

Sıklık:

Üç Yıllık

eISSN:

2667-467X

pISSN:

1300-5413

Özet:

 


It is well known that sensitivity of ICP-OES is not enough when ultra trace levels are concerned. From this perspective a new method was developed for the determinations of Cd(II) and Cu(II). It was based on the use of thermophilic Bacillus firmus loaded Amberlite XAD-4 as a biosorbent for solid phase extraction. The optimum pH, flow rate, eluent concentration and volume, amount of biomass and amount of Amberlit XAD-4 were found as 5.5, 3 mL min-1, 5 mL 1 mol L-1 of HCl, 250 mg of biomass and 750 mg of Amberlite XAD-4, respectively for recovery of tested metal ions. Preconcentration factor was achieved as 35 for Cd(II) and 50 for Cu(II). Resuability of the column were tested and same column could be used for 35 cycle. LODs were calculated as 0.09 and 0.08 ng mL-1, respectively for Cd(II) and Cu(II). RSDs were found as lower than 5.9%. The developed method was successfully applied to food samples to determine analyte concentrations.

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Abstract: It is well known that sensitivity of ICP-OES is not enough when ultra trace levels are concerned. From this perspective a new method was developed for the determinations of Cd(II) and Cu(II). It was based on the use of thermophilic Bacillus firmus loaded Amberlite XAD-4 as a biosorbent for solid phase extraction. The optimum pH, flow rate, eluent concentration and volume, amount of biomass and amount of Amberlit XAD-4 were found as 5.5, 3 mL min-1, 5 mL of 1 mol L-1 of HCl, 250 mg of biomass and 750 mg of Amberlite XAD-4, respectively for recovery of tested metal ions. Preconcentration factor was achieved as 35 for Cd and 50 for Cu. Resuability of the column were tested and same column could be used for 35 cycle. LODs were calculated as 0.09 and 0.08 ng mL-1, respectively for Cd and Cu. RSDs were found as lower than 5.9%. The developed method was successfully applied to food samples to determine analyte concentrations.

 

Özet: Eser elemenler söz konusu olduğunda ICP-OES'in hassasiyetinin yeterli olmadığı iyi bilinmektedir. Bu bakımdan Cd(II) ve Cu(II)'nin belirlenmesi için yeni bir metod geliştirilmiştir. Amberlit XAD-4 üzerine tutturulmuş termofilik Bacillus firmus temel baz alınarak katı faz ekstraksiyon için biyosorbent olarak kullanılmıştır. Test edilen metal iyonlarının geri kazanımı için optimum pH, akış hızı, eluent konsantrasyonu ve hacmi, biyokütle miktarı ve Amberlit XAD-4 miktarı sırasıyla; 5.5, 3 ml dk-1, 1 mol L-1 5 mL HCl, 250 mg ve 750 mg olarak tespit edildi. Prekonsantrasyon faktörü Cd için 35 ve Cu için 50 olarak elde edildi. Kolonun geri kullanımı test edildi ve aynı kolon 35 defa kullanılabilir. LODs Cd ve Cu için sırasıyla  0.09 and 0.08 ng mL-1 olarak hesaplandı. RSDs'nin %5.9'dan daha düşük olarak bulundu. Geliştirlen yöntem gıda örneklerindeki analit konsantrasyonlarının belirlenmesi için başarılı bir şekilde uygulandı.

 

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Makale detayları

Preconcentrations of Cd(II) and Cu(II) by solid phase extraction on thermophilic Bacillus firmus loaded Amberlite XAD-4 prior to their determinationsin food samples by ICP-OES

Yazarlar: Sadin ÖZDEMİR

Yayıncı: Van Yuzuncu Yıl University

Dergi: Yuzuncu Yil University Journal of the Institute of Natural and Applied Sciences 2016 Vol.21 Issue 2, pp.152-166

Belge Tipi: Makale

Yayın Tarihi: 12/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000757001

Sıklık: Üç Yıllık

eISSN: 2667-467X

pISSN: 1300-5413

Sayı

Yazarlar:

Sadin ÖZDEMİR

Yayıncı:

Van Yuzuncu Yıl University

Dergi:

Yuzuncu Yil University Journal of the Institute of Natural and Applied Sciences 2016 Vol.21 Issue 2, pp.152-166

Belge Tipi:

Makale

Yayın Tarihi:

12/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000757001

Sıklık:

Üç Yıllık

eISSN:

2667-467X

pISSN:

1300-5413