The Effects of Smoke and Smoke-Derived Compounds on Seed Germination of Liquidambar orientalis Mill. (Anatolian Sweetgum Tree)

Şükrü Serter Çatav; Kenan Akbaş

Makale detayları

The Effects of Smoke and Smoke-Derived Compounds on Seed Germination of Liquidambar orientalis Mill. (Anatolian Sweetgum Tree)

Yazarlar:

Şükrü Serter Çatav | Kenan Akbaş

Yayıncı:

Igdir University

Ana Başlıklar:

Ekoloji (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2393-2402

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001302951

pISSN:

2536-4618

Anahtar kelimeler:

Özet:

 Smoke and smoke-derived compounds can regulate physiological and developmental processes, such as seed germination, somatic embryogenesis, and flowering in plants. Smoke-related germination responses are highly complex due to the presence of stimulatory and inhibitory compounds, such as karrikins, glyceronitrile, syringaldehyde, catechol, and 3,4,5-trimethylfuran-25H-one. In this study, the effects of smoke water, karrikinolide KAR1, mandelonitrile MAN, and catechol CAT treatments on seed germination of Liquidambar orientalis Mill. were investigated. Moreover, gibberellic acid GA3 and reactive oxygen species ROS, which are thought to play a role in KAR1- and cyanohydrin-induced seed germination, were also included in the experiments. Hydrogen peroxide and methyl viologen solutions were used for ROS treatments. Germination experiments were carried out under different light conditions in incubators set at 20 C. The results of the study showed that L. orientalis seeds were sensitive to smoke. In addition, 0.01 and 0.1 µM KAR1 and 50 µM MAN solutions promoted the germination of the study species under both dark and light conditions p  0.05. On the other hand, CAT treatments did not have a positive or negative effect on germination. L. orientalis seeds sensitive to KAR1 and cyanohydrin gave a positive germination response to GA3 in the concentration range of 10-5 to 10-3 M. GA3 treatments also eliminated the requirement of light for high germination success. ROS-induced germination was only observed in the presence of light. The findings of this study can be used to increase the germination success of L. orientalis, a narrowly distributed species.

 Smoke and smoke-derived compounds can regulate physiological and developmental processes, such as seed germination, somatic embryogenesis, and flowering in plants. Smoke-related germination responses are highly complex due to the presence of stimulatory and inhibitory compounds, such as karrikins, glyceronitrile, syringaldehyde, catechol, and 3,4,5-trimethylfuran-25H-one. In this study, the effects of smoke water, karrikinolide KAR1, mandelonitrile MAN, and catechol CAT treatments on seed germination of Liquidambar orientalis Mill. were investigated. Moreover, gibberellic acid GA3 and reactive oxygen species ROS, which are thought to play a role in KAR1- and cyanohydrin-induced seed germination, were also included in the experiments. Hydrogen peroxide and methyl viologen solutions were used for ROS treatments. Germination experiments were carried out under different light conditions in incubators set at 20 C. The results of the study showed that L. orientalis seeds were sensitive to smoke. In addition, 0.01 and 0.1 µM KAR1 and 50 µM MAN solutions promoted the germination of the study species under both dark and light conditions p  0.05. On the other hand, CAT treatments did not have a positive or negative effect on germination. L. orientalis seeds sensitive to KAR1 and cyanohydrin gave a positive germination response to GA3 in the concentration range of 10-5 to 10-3 M. GA3 treatments also eliminated the requirement of light for high germination success. ROS-induced germination was only observed in the presence of light. The findings of this study can be used to increase the germination success of L. orientalis, a narrowly distributed species.

 Smoke and smoke-derived compounds can regulate physiological and developmental processes, such as seed germination, somatic embryogenesis, and flowering in plants. Smoke-related germination responses are highly complex due to the presence of stimulatory and inhibitory compounds, such as karrikins, glyceronitrile, syringaldehyde, catechol, and 3,4,5-trimethylfuran-25H-one. In this study, the effects of smoke water, karrikinolide KAR1, mandelonitrile MAN, and catechol CAT treatments on seed germination of Liquidambar orientalis Mill. were investigated. Moreover, gibberellic acid GA3 and reactive oxygen species ROS, which are thought to play a role in KAR1- and cyanohydrin-induced seed germination, were also included in the experiments. Hydrogen peroxide and methyl viologen solutions were used for ROS treatments. Germination experiments were carried out under different light conditions in incubators set at 20 C. The results of the study showed that L. orientalis seeds were sensitive to smoke. In addition, 0.01 and 0.1 µM KAR1 and 50 µM MAN solutions promoted the germination of the study species under both dark and light conditions p  0.05. On the other hand, CAT treatments did not have a positive or negative effect on germination. L. orientalis seeds sensitive to KAR1 and cyanohydrin gave a positive germination response to GA3 in the concentration range of 10-5 to 10-3 M. GA3 treatments also eliminated the requirement of light for high germination success. ROS-induced germination was only observed in the presence of light. The findings of this study can be used to increase the germination success of L. orientalis, a narrowly distributed species.

 Duman ve duman kökenli bileşikler, bitkilerde tohum çimlenmesi, somatik embriyogenez ve çiçeklenme gibi fizyolojik ve gelişimsel süreçleri düzenleyebilmektedir. Dumanla ilişkili çimlenme yanıtları, karrikinler, gliseronitril, siringaldehit, katekol ve 3,4,5-trimetilfuran-25H-on gibi uyarıcı ve inhibe edici bileşiklerin mevcudiyeti nedeniyle oldukça karmaşık bir yapıya sahiptir. Bu çalışmada, duman suyu, karrikinolid KAR1, mandelonitril MAN ve katekol KAT uygulamalarının Liquidambar orientalis Mill.in tohum çimlenmesi üzerindeki etkileri incelenmiştir. Ayrıca KAR1 ve siyanohidrin ile indüklenen tohum çimlenmesinde rol aldıkları düşünülen giberellik asit GA3 ve reaktif oksijen türleri de ROT deneylere dâhil edilmiştir. ROT uygulamaları için hidrojen peroksit ve metil violojen çözeltileri kullanılmıştır. Çimlenme deneyleri, 20 Cye ayarlanmış inkübatörlerde farklı ışık koşulları altında gerçekleştirilmiştir. Çalışmanın sonuçları, L. orientalis tohumlarının dumana karşı duyarlı olduğunu göstermiştir. Buna ek olarak, 0.01 ve 0.1 µM KAR1 ve 50 µM MAN solüsyonları hem karanlık hem de aydınlık koşullar altında çalışma türünün çimlenmesini teşvik etmiştir p  0.05. Öte yandan, KAT uygulamaları çimlenme üzerinde olumlu veya olumsuz bir etki yaratmamıştır. KAR1 ve siyanohidrine hassas olan L. orientalis tohumları 10-5 ila 10-3 M konsantrasyon aralığında GA3e karşı pozitif bir çimlenme cevabı vermiştir. GA3 uygulamaları yüksek çimlenme başarısı için ışığa olan gereksinimi de ortadan kaldırmıştır. ROT ile uyarılan çimlenme ise sadece ışık varlığında gözlemlenmiştir. Bu çalışmadan elde edilen bulgular, dar yayılışlı bir tür olan L. orientalisin çimlenme başarısını arttırmak için kullanılabilir.

Makale detayları

The Effects of Smoke and Smoke-Derived Compounds on Seed Germination of Liquidambar orientalis Mill. (Anatolian Sweetgum Tree)

Yazarlar:

Şükrü Serter Çatav | Kenan Akbaş

Yayıncı:

Igdir University

Ana Başlıklar:

Ekoloji (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2393-2402

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001302951

pISSN:

2536-4618

Smoke-water

Karrikinolide

Gibberellic acid

Reactive oxygen species

CyanohydrinDuman suyu

Karrikinolid

Giberellik asit

Reaktif oksijen türleri

Siyanohidrin

Sayı

Makale detayları

Yazarlar: Şükrü Serter Çatav | Kenan Akbaş

Yayıncı: Igdir University

Ana Başlıklar: Ekoloji (Diğer)

Dergi: Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2393-2402

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001302951

pISSN: 2536-4618

Sayı

Yazarlar:

Şükrü Serter Çatav | Kenan Akbaş

Yayıncı:

Igdir University

Ana Başlıklar:

Ekoloji (Diğer)

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2393-2402

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001302951

pISSN:

2536-4618