Effect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells

N.A.

Makale detayları

Effect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Clinical Sciences

Dergi:

Marmara Medical Journal Vol.36 Issue 1, pp.39-45

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283559

pISSN:

1019-1941

Anahtar kelimeler:

Özet:

 Objective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined their effects on MCF-7 cells. Materials and Methods: Whey proteins obtained from 6 whey powder were treated with hazelnut oil HO and olive oil OO at a protein to lipid ratio of 1:10 at 60 C for 120 minutes. The protein derivatives formed with whey protein and HO or OO were applied to MCF-7 cancer cells and healthy fibroblasts. The effects of protein derivatives on cell viability, apoptosis, reactive oxygen species ROS production, wound healing, cell cycle phase distribution and cell cycle related proteins Akt and p21Waf1Cip1 expressions were investigated. Results: Cell viability decreased significantly after 24 h of incubation with WP:OO. The percentage of apoptotic or necrotic cells varied between 5-10 and no statistically significant effect was observed. There was no statistically significant difference in ROS production and colony formation between controls and WP:HO or WP:OO groups. Treatment of cells with WP:OO for 24 h significantly decreased cell migration compared to the control group. G2M phase was significantly suppressed in WP:OO group compared to the control group. WP:OO also increased the expression of p21Waf1Cip1 significantly when compared with the control group. Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer treatment.

 Objective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined their effects on MCF-7 cells. Materials and Methods: Whey proteins obtained from 6 whey powder were treated with hazelnut oil HO and olive oil OO at a protein to lipid ratio of 1:10 at 60 C for 120 minutes. The protein derivatives formed with whey protein and HO or OO were applied to MCF-7 cancer cells and healthy fibroblasts. The effects of protein derivatives on cell viability, apoptosis, reactive oxygen species ROS production, wound healing, cell cycle phase distribution and cell cycle related proteins Akt and p21Waf1Cip1 expressions were investigated. Results: Cell viability decreased significantly after 24 h of incubation with WP:OO. The percentage of apoptotic or necrotic cells varied between 5-10 and no statistically significant effect was observed. There was no statistically significant difference in ROS production and colony formation between controls and WP:HO or WP:OO groups. Treatment of cells with WP:OO for 24 h significantly decreased cell migration compared to the control group. G2M phase was significantly suppressed in WP:OO group compared to the control group. WP:OO also increased the expression of p21Waf1Cip1 significantly when compared with the control group. Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer treatment.

 Objective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined their effects on MCF-7 cells. Materials and Methods: Whey proteins obtained from 6 whey powder were treated with hazelnut oil HO and olive oil OO at a protein to lipid ratio of 1:10 at 60 C for 120 minutes. The protein derivatives formed with whey protein and HO or OO were applied to MCF-7 cancer cells and healthy fibroblasts. The effects of protein derivatives on cell viability, apoptosis, reactive oxygen species ROS production, wound healing, cell cycle phase distribution and cell cycle related proteins Akt and p21Waf1Cip1 expressions were investigated. Results: Cell viability decreased significantly after 24 h of incubation with WP:OO. The percentage of apoptotic or necrotic cells varied between 5-10 and no statistically significant effect was observed. There was no statistically significant difference in ROS production and colony formation between controls and WP:HO or WP:OO groups. Treatment of cells with WP:OO for 24 h significantly decreased cell migration compared to the control group. G2M phase was significantly suppressed in WP:OO group compared to the control group. WP:OO also increased the expression of p21Waf1Cip1 significantly when compared with the control group. Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer treatment.

 Objective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined their effects on MCF-7 cells. Materials and Methods: Whey proteins obtained from 6 whey powder were treated with hazelnut oil HO and olive oil OO at a protein to lipid ratio of 1:10 at 60 C for 120 minutes. The protein derivatives formed with whey protein and HO or OO were applied to MCF-7 cancer cells and healthy fibroblasts. The effects of protein derivatives on cell viability, apoptosis, reactive oxygen species ROS production, wound healing, cell cycle phase distribution and cell cycle related proteins Akt and p21Waf1Cip1 expressions were investigated. Results: Cell viability decreased significantly after 24 h of incubation with WP:OO. The percentage of apoptotic or necrotic cells varied between 5-10 and no statistically significant effect was observed. There was no statistically significant difference in ROS production and colony formation between controls and WP:HO or WP:OO groups. Treatment of cells with WP:OO for 24 h significantly decreased cell migration compared to the control group. G2M phase was significantly suppressed in WP:OO group compared to the control group. WP:OO also increased the expression of p21Waf1Cip1 significantly when compared with the control group. Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer treatment.

Makale detayları

Effect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Clinical Sciences

Dergi:

Marmara Medical Journal Vol.36 Issue 1, pp.39-45

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283559

pISSN:

1019-1941

Whey proteins

Oleic acid

Cell survival

Apoptosis

Cell cycle

Whey proteins

Oleic acid

Cell survival

Apoptosis

Cell cycle

Sayı

Makale detayları

Yazarlar: N.A.

Yayıncı: Marmara University

Ana Başlıklar: Clinical Sciences

Dergi: Marmara Medical Journal Vol.36 Issue 1, pp.39-45

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001283559

pISSN: 1019-1941

Sayı

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Clinical Sciences

Dergi:

Marmara Medical Journal Vol.36 Issue 1, pp.39-45

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283559

pISSN:

1019-1941