Peroxisome proliferate activator receptor-γ (PPAR-γ) gene  expression and global DNA methylation as predictors for  insulin resistance in healthy adults

N.A.

Makale detayları

Peroxisome proliferate activator receptor-γ (PPAR-γ) gene expression and global DNA methylation as predictors for insulin resistance in healthy adults

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Toxicology

Dergi:

Journal of Research in Pharmacy Vol.29 Issue 1, pp.272-279

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001339613

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Finding a feasible test for screening for insulin resistance IR in healthy adults can significantly reduce the incidence of plethora of metabolic and cardiovascular diseases and for this reason the current study was aimed to evaluate the prognostic value of peroxisome proliferator-activated receptor γ PPAR-γ gene expression and global DNA methylation in prediction of IR in healthy adults in a cross-sectional study conducted on 100 euglycemic, non-diabetic apparently healthy adults of both sexes, aged 45 years old with HbA1C 6.5, who were categorized into insulin sensitive IS and IR according to Homeostatic Model Assessment of IR HOMA-IR. Global DNA methylation was estimated with enzyme linked immune-sorbent assay using a ready commercial kit. Quantitative polymerase chain reaction qPCR was used to estimate PPAR-γ gene expression in relation reference gene and the results revealed that out of 100 apparently healthy subjects, 30 subjects 30 had IR. The Ct of PPARγ gene was significantly lower in the IR individuals 6.492.46 in comparison to 8.131.37 of the IS individuals. However, it has relative low sensitivity and specificity 56 and 53, respectively. There was no significant difference between individual with and without IR in the intensity of global DNA methylation that may lead to conclude that PPAR-γ gene expression is significantly reduced in individuals with IR; however, it cannot be used as a screening test for IR, due to low sensitivity and specificity. Global DNA methylation seems to have no association with IR.

 Finding a feasible test for screening for insulin resistance IR in healthy adults can significantly reduce the incidence of plethora of metabolic and cardiovascular diseases and for this reason the current study was aimed to evaluate the prognostic value of peroxisome proliferator-activated receptor γ PPAR-γ gene expression and global DNA methylation in prediction of IR in healthy adults in a cross-sectional study conducted on 100 euglycemic, non-diabetic apparently healthy adults of both sexes, aged 45 years old with HbA1C 6.5, who were categorized into insulin sensitive IS and IR according to Homeostatic Model Assessment of IR HOMA-IR. Global DNA methylation was estimated with enzyme linked immune-sorbent assay using a ready commercial kit. Quantitative polymerase chain reaction qPCR was used to estimate PPAR-γ gene expression in relation reference gene and the results revealed that out of 100 apparently healthy subjects, 30 subjects 30 had IR. The Ct of PPARγ gene was significantly lower in the IR individuals 6.492.46 in comparison to 8.131.37 of the IS individuals. However, it has relative low sensitivity and specificity 56 and 53, respectively. There was no significant difference between individual with and without IR in the intensity of global DNA methylation that may lead to conclude that PPAR-γ gene expression is significantly reduced in individuals with IR; however, it cannot be used as a screening test for IR, due to low sensitivity and specificity. Global DNA methylation seems to have no association with IR.

 Finding a feasible test for screening for insulin resistance IR in healthy adults can significantly reduce the incidence of plethora of metabolic and cardiovascular diseases and for this reason the current study was aimed to evaluate the prognostic value of peroxisome proliferator-activated receptor γ PPAR-γ gene expression and global DNA methylation in prediction of IR in healthy adults in a cross-sectional study conducted on 100 euglycemic, non-diabetic apparently healthy adults of both sexes, aged 45 years old with HbA1C 6.5, who were categorized into insulin sensitive IS and IR according to Homeostatic Model Assessment of IR HOMA-IR. Global DNA methylation was estimated with enzyme linked immune-sorbent assay using a ready commercial kit. Quantitative polymerase chain reaction qPCR was used to estimate PPAR-γ gene expression in relation reference gene and the results revealed that out of 100 apparently healthy subjects, 30 subjects 30 had IR. The Ct of PPARγ gene was significantly lower in the IR individuals 6.492.46 in comparison to 8.131.37 of the IS individuals. However, it has relative low sensitivity and specificity 56 and 53, respectively. There was no significant difference between individual with and without IR in the intensity of global DNA methylation that may lead to conclude that PPAR-γ gene expression is significantly reduced in individuals with IR; however, it cannot be used as a screening test for IR, due to low sensitivity and specificity. Global DNA methylation seems to have no association with IR.

 Finding a feasible test for screening for insulin resistance IR in healthy adults can significantly reduce the incidence of plethora of metabolic and cardiovascular diseases and for this reason the current study was aimed to evaluate the prognostic value of peroxisome proliferator-activated receptor γ PPAR-γ gene expression and global DNA methylation in prediction of IR in healthy adults in a cross-sectional study conducted on 100 euglycemic, non-diabetic apparently healthy adults of both sexes, aged 45 years old with HbA1C 6.5, who were categorized into insulin sensitive IS and IR according to Homeostatic Model Assessment of IR HOMA-IR. Global DNA methylation was estimated with enzyme linked immune-sorbent assay using a ready commercial kit. Quantitative polymerase chain reaction qPCR was used to estimate PPAR-γ gene expression in relation reference gene and the results revealed that out of 100 apparently healthy subjects, 30 subjects 30 had IR. The Ct of PPARγ gene was significantly lower in the IR individuals 6.492.46 in comparison to 8.131.37 of the IS individuals. However, it has relative low sensitivity and specificity 56 and 53, respectively. There was no significant difference between individual with and without IR in the intensity of global DNA methylation that may lead to conclude that PPAR-γ gene expression is significantly reduced in individuals with IR; however, it cannot be used as a screening test for IR, due to low sensitivity and specificity. Global DNA methylation seems to have no association with IR.

Makale detayları

Peroxisome proliferate activator receptor-γ (PPAR-γ) gene expression and global DNA methylation as predictors for insulin resistance in healthy adults

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Toxicology

Dergi:

Journal of Research in Pharmacy Vol.29 Issue 1, pp.272-279

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001339613

pISSN:

2630-6344

Global DNA methylation

homeostatic model assessment

insulin resistance

peroxisome proliferate activator receptor-γ gene

type 2 diabetes

Sayı

Makale detayları

Peroxisome proliferate activator receptor-γ (PPAR-γ) gene expression and global DNA methylation as predictors for insulin resistance in healthy adults

Yazarlar: N.A.

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Toxicology

Dergi: Journal of Research in Pharmacy Vol.29 Issue 1, pp.272-279

Belge Tipi: Makale

Yayın Tarihi: 3/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001339613

pISSN: 2630-6344

Global DNA methylation

homeostatic model assessment

insulin resistance

peroxisome proliferate activator receptor-γ gene

type 2 diabetes

Sayı

Yazarlar:

N.A.

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Toxicology

Dergi:

Journal of Research in Pharmacy Vol.29 Issue 1, pp.272-279

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001339613

pISSN:

2630-6344