Partial Purification of Glutathione S-Transferase Enzyme from MuscleTissue of Scorpionfish

Kübra Işık; Ercan Soydan

Makale detayları

Partial Purification of Glutathione S-Transferase Enzyme from MuscleTissue of Scorpionfish

Yazarlar:

Kübra Işık | Ercan Soydan

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Tarımda Enzim ve Mikrobiyal Biyoteknoloji

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 2, pp.431-440

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001291425

pISSN:

1308-8750

Anahtar kelimeler:

Özet:

 Enzymes serve to make vital functions healthy and sustainable in living organisms. GST enzyme, which is one of the most important enzymes that provide such a resistance in human body, provides the detoxification of xenobiotics and is a well-known enzyme in the detoxification of xenobiotic substances. Therefore, GSTs convert endogenous substances produced by oxidative stress and xenobiotics such as antitumour drugs and environmental carcinogenics, into dissoluble form via phase II transformation. In this study, glutathione S-transferase enzyme from muscle tissue of scorpion fish was partially purified for the first time in the literature and optimum characterization studies were performed. The working procedure was carried out in three steps as homogenate preparation, ammonium sulfate precipitation and dialysis, respectively. Ammonium sulfate precipitation interval was found in the range of 40-60. Optimum ionic strength was determined in 150 mM Tris-HCl buffer, optimum pH value was found to be 6.0 and optimum substrate concentration was determined as 2.5 mM CDNB. Despite studies involved in characterization of antioxidant enzymes from aquatic organisms are quite common, results of this study are considered to be beneficial for scientists studying on this research area.

 Enzymes serve to make vital functions healthy and sustainable in living organisms. GST enzyme, which is one of the most important enzymes that provide such a resistance in human body, provides the detoxification of xenobiotics and is a well-known enzyme in the detoxification of xenobiotic substances. Therefore, GSTs convert endogenous substances produced by oxidative stress and xenobiotics such as antitumour drugs and environmental carcinogenics, into dissoluble form via phase II transformation. In this study, glutathione S-transferase enzyme from muscle tissue of scorpion fish was partially purified for the first time in the literature and optimum characterization studies were performed. The working procedure was carried out in three steps as homogenate preparation, ammonium sulfate precipitation and dialysis, respectively. Ammonium sulfate precipitation interval was found in the range of 40-60. Optimum ionic strength was determined in 150 mM Tris-HCl buffer, optimum pH value was found to be 6.0 and optimum substrate concentration was determined as 2.5 mM CDNB. Despite studies involved in characterization of antioxidant enzymes from aquatic organisms are quite common, results of this study are considered to be beneficial for scientists studying on this research area.

 Enzymes serve to make vital functions healthy and sustainable in living organisms. GST enzyme, which is one of the most important enzymes that provide such a resistance in human body, provides the detoxification of xenobiotics and is a well-known enzyme in the detoxification of xenobiotic substances. Therefore, GSTs convert endogenous substances produced by oxidative stress and xenobiotics such as antitumour drugs and environmental carcinogenics, into dissoluble form via phase II transformation. In this study, glutathione S-transferase enzyme from muscle tissue of scorpion fish was partially purified for the first time in the literature and optimum characterization studies were performed. The working procedure was carried out in three steps as homogenate preparation, ammonium sulfate precipitation and dialysis, respectively. Ammonium sulfate precipitation interval was found in the range of 40-60. Optimum ionic strength was determined in 150 mM Tris-HCl buffer, optimum pH value was found to be 6.0 and optimum substrate concentration was determined as 2.5 mM CDNB. Despite studies involved in characterization of antioxidant enzymes from aquatic organisms are quite common, results of this study are considered to be beneficial for scientists studying on this research area.

 Canlı organizmalarda enzimlerin görevleri yaşamsal fonksiyonları sağlıklı ve sürdürülebilir hale getirmektir. Vücudumuz için bu direnci sağlayan enzimlerin en önemlilerinden biri olan glutatyon S-transferaz enzimi ksenobiyotiklerin detoksifikasyonunu sağlamaktadır ve ksenobiyotik maddelerin detoksifikasyonundaki fonksiyonu iyi bilinen bir enzimdir. Bu nedenle bu enzim oksidatif stres sonucu oluşan endojen bileşiklerin ve antitümör ilaçlar, çevresel karsinojenler gibi ksenobiyotiklerin faz II aşamasıyla transformasyonunu sağlayarak ksenobiyotikleri çözünür hale getirmektedir. Bu çalışmada iskorpit balığı kas dokusundan glutatyon S-transferaz enzimi literatürde ilk kez kısmi olarak saflaştırılmış ve kinetik karakterizasyon çalışmaları yapılmıştır. Çalışma prosedürü işlemi sırasıyla homojenat hazırlama, amonyum sülfat çöktürmesi ve diyaliz olarak üç aşamada yapılmıştır. Amonyum sülfat çöktürme aralığı 40-60 olarak bulunmuştur. Optimum karakterizasyon çalışmaları yapılarak optimum iyonik şiddeti 150 mM Tris-HCI tamponunda, optimum pH: 6.0 ve optimum substrat miktarı ise 2.5 mM CDNB olarak tespit edilmiştir. Akuatik canlılardan antioksidant enzimlerin karakterizasyonu çalışmaları oldukça yaygın olmasına rağmen çalışmamızın sonuçlarının bu alanda araştırmalar yapan bilim insanları için faydalı olacağı düşünülmektedir.

Makale detayları

Partial Purification of Glutathione S-Transferase Enzyme from MuscleTissue of Scorpionfish

Yazarlar:

Kübra Işık | Ercan Soydan

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Tarımda Enzim ve Mikrobiyal Biyoteknoloji

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 2, pp.431-440

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001291425

pISSN:

1308-8750

Enzyme purification

glutathione S-transferase

characterization study

scorpion fishEnzim saflaştırılması

glutatyon S-transferaz

iskorpit balığı

karakterizasyon çalışması

Sayı

Makale detayları

Yazarlar: Kübra Işık | Ercan Soydan

Yayıncı: Ondokuz Mayıs University

Ana Başlıklar: Tarımda Enzim ve Mikrobiyal Biyoteknoloji

Dergi: Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 2, pp.431-440

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001291425

pISSN: 1308-8750

Sayı

Yazarlar:

Kübra Işık | Ercan Soydan

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Tarımda Enzim ve Mikrobiyal Biyoteknoloji

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 2, pp.431-440

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001291425

pISSN:

1308-8750