Herpes Simplex Encephalitis Cases with Typical and Atypical Symptoms Confirmed by PCR-Am- plification of the DNA Polymerase Gene

G.m. MANTAR; W.a. KHOUDOUD; M. FAYAD; R. CHOUEİRİ; M. MİKATİ; A.m. ABDELNOOR

Makale detayları

Herpes Simplex Encephalitis Cases with Typical and Atypical Symptoms Confirmed by PCR-Am- plification of the DNA Polymerase Gene

Yazarlar:

G.m. MANTAR | W.a. KHOUDOUD | M. FAYAD | R. CHOUEİRİ | M. MİKATİ | A.m. ABDELNOOR

Yayıncı:

Van Yuzuncu Yıl University

Dergi:

EASTERN JOURNAL OF MEDICINE 2000 Vol.5 Issue 2, pp.70-78

Belge Tipi:

Makale

Yayın Tarihi:

3/2003

Belge Dili:

İngilizce

Document ID (Org No.):

2000773608

Sıklık:

Aylık

eISSN:

1301-0883

pISSN:

1301-0883

Özet:

 

Objective: We have confirmed by PCR-amplification ofthe DNA polymerase gene, three cases of Herpes Sim-plex encephalitis (HSE) with atypical clinical symp-toms.Methods: Cerebrospinal fluid (CSF) samples were col-lected by lumbar puncture during the course of test-ing from 23 patients suspected to have viral encepha-litis based on commonly encountered symptoms, cy-tochemical analysis and neurological testing. PCR wasdone on CSF using specific primers that amplify a 290base pair sequence on the DNA polymerase gene com-mon to both HSV-1 and HSV-2 strains.Results:  Only 3 of the 23 CSF samples collectedshowed the expected amplicon size on the target se-quence. One case had conventional symptoms of en-cephalitis and 2 cases showed atypical symptoms fromthe conventional ones. All 3 patients recovered afteradministration of acyclovir. Fourteen PCR-negativepatients were shown to have non-HSE viral encephali-tis and the remaining 6 patients were shown later tohave other neurological diseases.Conclusion:   PCR was efficient in elucidating the atypi-cal cases of HSV encephalitis.Key words: HSV, encephalitis, PCR.

Makale detayları

Herpes Simplex Encephalitis Cases with Typical and Atypical Symptoms Confirmed by PCR-Am- plification of the DNA Polymerase Gene

Yazarlar: G.m. MANTAR | W.a. KHOUDOUD | M. FAYAD | R. CHOUEİRİ | M. MİKATİ | A.m. ABDELNOOR

Yayıncı: Van Yuzuncu Yıl University

Dergi: EASTERN JOURNAL OF MEDICINE 2000 Vol.5 Issue 2, pp.70-78

Belge Tipi: Makale

Yayın Tarihi: 3/2003

Belge Dili: İngilizce

Document ID (Org No.): 2000773608

Sıklık: Aylık

eISSN: 1301-0883

pISSN: 1301-0883

Sayı

Yazarlar:

G.m. MANTAR | W.a. KHOUDOUD | M. FAYAD | R. CHOUEİRİ | M. MİKATİ | A.m. ABDELNOOR

Yayıncı:

Van Yuzuncu Yıl University

Dergi:

EASTERN JOURNAL OF MEDICINE 2000 Vol.5 Issue 2, pp.70-78

Belge Tipi:

Makale

Yayın Tarihi:

3/2003

Belge Dili:

İngilizce

Document ID (Org No.):

2000773608

Sıklık:

Aylık

eISSN:

1301-0883

pISSN:

1301-0883