L-arginine protects against ethylene glycol-induced gastric mucosal damage in rats: immunohistochemical and electron microscopic study

Samah KANDEEL; Thoryaa EL-DEEB; Ehsan SALAH; Nafisa EL-BAKARY; Naglaa SARHAN

Makale detayları

L-arginine protects against ethylene glycol-induced gastric mucosal damage in rats: immunohistochemical and electron microscopic study

Yazarlar:

Samah KANDEEL | Thoryaa EL-DEEB | Ehsan SALAH | Nafisa EL-BAKARY | Naglaa SARHAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.342-349

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812453

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Ethylene glycol is an alcohol widely used in industry as an antifreeze. It affects the normal gastric mucosal defense mechanisms, inducing surface ulcerations. L-arginine is a precursor of nitric oxide that exhibits multiple biological properties; among them is the acceleration of wound healing, especially in the stomach. Caspase-3 immunohistochemistry, and semithin and ultrathin section examinations were used to investigate the possible protective effect of L-arginine on ethylene glycol-induced gastric mucosal damage in rats. Three animal groups were used: group A (control group), group B (received 3.3 mL/kg ethylene glycol for 2 weeks), and group C (received 200 mg/kg of L-arginine and 3.3 mL/kg ethylene glycol for 2 weeks). By examination, group B showed significant increases in caspase-3 positive cells in comparison to the control, semithin sections revealed surface disruption and ulcerations, and ultrathin sections showed multiple pathological changes in cells lining the gastric mucosal glands. In group C significant decreases in caspase-3 positive cells were encountered and the other pathological findings in group B were found to be reversed. Therefore, we conclude that ethylene glycol-induced damage to the gastric mucosa could be prevented by L-arginine administration.

 

Ethylene glycol is an alcohol widely used in industry as an antifreeze. It affects the normal gastric mucosal defense mechanisms, inducing surface ulcerations. L-arginine is a precursor of nitric oxide that exhibits multiple biological properties; among them is the acceleration of wound healing, especially in the stomach. Caspase-3 immunohistochemistry, and semithin and ultrathin section examinations were used to investigate the possible protective effect of L-arginine on ethylene glycol-induced gastric mucosal damage in rats. Three animal groups were used: group A (control group), group B (received 3.3 mL/kg ethylene glycol for 2 weeks), and group C (received 200 mg/kg of L-arginine and 3.3 mL/kg ethylene glycol for 2 weeks). By examination, group B showed significant increases in caspase-3 positive cells in comparison to the control, semithin sections revealed surface disruption and ulcerations, and ultrathin sections showed multiple pathological changes in cells lining the gastric mucosal glands. In group C significant decreases in caspase-3 positive cells were encountered and the other pathological findings in group B were found to be reversed. Therefore, we conclude that ethylene glycol-induced damage to the gastric mucosa could be prevented by L-arginine administration.

Makale detayları

L-arginine protects against ethylene glycol-induced gastric mucosal damage in rats: immunohistochemical and electron microscopic study

Yazarlar:

Samah KANDEEL | Thoryaa EL-DEEB | Ehsan SALAH | Nafisa EL-BAKARY | Naglaa SARHAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.342-349

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812453

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Ethylene glycol

L-arginine

stomach

caspase-3

immunohistochemistry

ultrastructure

rat

Sayı

Makale detayları

Yazarlar: Samah KANDEEL | Thoryaa EL-DEEB | Ehsan SALAH | Nafisa EL-BAKARY | Naglaa SARHAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.342-349

Belge Tipi: Makale

Yayın Tarihi: 2/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000812453

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Samah KANDEEL | Thoryaa EL-DEEB | Ehsan SALAH | Nafisa EL-BAKARY | Naglaa SARHAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 3, pp.342-349

Belge Tipi:

Makale

Yayın Tarihi:

2/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812453

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152