Effects of chronic unpredictable stress on intestinal morphology in Wistar rats

Ufuk ÇORUMLU; Osman Özcan AYDIN; Emel ULUPINAR

Makale detayları

Effects of chronic unpredictable stress on intestinal morphology in Wistar rats

Yazarlar:

Ufuk ÇORUMLU | Osman Özcan AYDIN | Emel ULUPINAR

Yayıncı:

Society of Anatomy and Clinical Anatomy

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Anatomy 2019 Vol.13 Issue 1, pp.27-32

Belge Tipi:

Makale

Yayın Tarihi:

4/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000668008

Sıklık:

Üç Yıllık

eISSN:

1308-8459

Özet:

 


Objectives: Stressful events might cause immune dysfunction and trigger various disorders. Adverse effects of acute or
chronic stress exposure on the gastrointestinal system have been shown previously in several studies. In this experimental
study, we used chronic unpredictable stress (CUS) paradigm to better mimic effects of the intermittent exposure to daily life
stress and investigated the morphometric alterations occurring in the small intestines of rats.


Methods: Male Wistar rats were randomly divided into stress and control groups (n=8, each). While stress group was subjected to chronic unpredictable stress protocol for 21 days, control group remained undisturbed. Intestinal tissue samples
were obtained from two different regions; one was 3-6 cm away from the pylorus and the other one 3-6 cm prior to the
ileocaecal valve. Tissue sections were obtained from paraffin blocks at the thickness of 3 micrometers and stained with
hematoxylin-eosin (HE) or periodic acid-Shiff (PAS). The lengths of villi were measured from the basal membrane to the top
of the villus. The ratio of degranulating and non-degranulating mast cells per unit area were estimated by point counting
method.


Results: The mean villi length in the stress group were significantly higher (p<0.01) than those of the control group.
Degranulation to non-degranulation ratio of the mast cells were 40% and 54% in the control and stress groups, respectively.

Conclusion: Animals exposed to chronic unpredictable stress protocol displayed a significant elongation in the villi of small
intestines and an increase in the number of degranulating mast cells in the intestinal mucosa. Since activation of mast cells
causes releasing of various chemical mediators and growth factors, it is plausible that stressed animals developed an adaptation mechanism to enhance the capacity for absorption and digestion per unit length of the guts. 

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Makale detayları

Effects of chronic unpredictable stress on intestinal morphology in Wistar rats

Yazarlar: Ufuk ÇORUMLU | Osman Özcan AYDIN | Emel ULUPINAR

Yayıncı: Society of Anatomy and Clinical Anatomy

Ana Başlıklar: Sağlık teşvikleri

Dergi: Anatomy 2019 Vol.13 Issue 1, pp.27-32

Belge Tipi: Makale

Yayın Tarihi: 4/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000668008

Sıklık: Üç Yıllık

eISSN: 1308-8459

Sayı

Yazarlar:

Ufuk ÇORUMLU | Osman Özcan AYDIN | Emel ULUPINAR

Yayıncı:

Society of Anatomy and Clinical Anatomy

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Anatomy 2019 Vol.13 Issue 1, pp.27-32

Belge Tipi:

Makale

Yayın Tarihi:

4/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000668008

Sıklık:

Üç Yıllık

eISSN:

1308-8459