Comparison of Ovine Spermatozoal Morphological Features After Staining of Fixation and Assessment of Morphological Abnormalities in Dead/Live Spermatozoa

Yavuz ÖZTÜRKLER

Makale detayları

Comparison of Ovine Spermatozoal Morphological Features After Staining of Fixation and Assessment of Morphological Abnormalities in Dead/Live Spermatozoa

Yazarlar:

Yavuz ÖZTÜRKLER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 5, pp.675-680

Belge Tipi:

Makale

Yayın Tarihi:

4/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000771143

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The objectives of this study were to (i) compare the effects of two staining techniques and one fixative solution on the morphological features of ram spermatozoa and (ii) evaluate the morphological features of dead/live spermatozoa by using Eosin-Nigrosin staining. The morphological features of raw spermatozoa were evaluated (by Phase Contrast microscopy) after staining with Eosin-Nigrosin mixture or Nigrosin (alone), or after fixation with Hancock's buffered formol saline solution. 42 raw pooled semen samples from 6 Kıvırcık rams were used. The percentages of spermatozoa with abnormal acrosomes and total morphological abnormalities averaged 3.97% and 14.78% in Hancock's solution, compared with averages of 10.07% and 18.54%; 7.97% and 18.45% for smears stained with Eosin-Nigrosin or Nigrosin (alone), respectively (P.

 

The objectives of this study were to (i) compare the effects of two staining techniques and one fixative solution on the morphological features of ram spermatozoa and (ii) evaluate the morphological features of dead/live spermatozoa by using Eosin-Nigrosin staining. The morphological features of raw spermatozoa were evaluated (by Phase Contrast microscopy) after staining with Eosin-Nigrosin mixture or Nigrosin (alone), or after fixation with Hancock's buffered formol saline solution. 42 raw pooled semen samples from 6 Kıvırcık rams were used. The percentages of spermatozoa with abnormal acrosomes and total morphological abnormalities averaged 3.97% and 14.78% in Hancock's solution, compared with averages of 10.07% and 18.54%; 7.97% and 18.45% for smears stained with Eosin-Nigrosin or Nigrosin (alone), respectively (P.

Makale detayları

Comparison of Ovine Spermatozoal Morphological Features After Staining of Fixation and Assessment of Morphological Abnormalities in Dead/Live Spermatozoa

Yazarlar:

Yavuz ÖZTÜRKLER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 5, pp.675-680

Belge Tipi:

Makale

Yayın Tarihi:

4/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000771143

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Ram

spermatozoa

fixation

staining

Sayı

Makale detayları

Comparison of Ovine Spermatozoal Morphological Features After Staining of Fixation and Assessment of Morphological Abnormalities in Dead/Live Spermatozoa

Yazarlar: Yavuz ÖZTÜRKLER

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 5, pp.675-680

Belge Tipi: Makale

Yayın Tarihi: 4/2001

Belge Dili: Türkçe

Document ID (Org No.): 2000771143

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Ram

spermatozoa

fixation

staining

Sayı

Yazarlar:

Yavuz ÖZTÜRKLER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 5, pp.675-680

Belge Tipi:

Makale

Yayın Tarihi:

4/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000771143

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128