Levels of leukocyte oxidative DNA damage (8-OHdG), serum coenzyme Q10 and lipid peroxidation in the formation attacks of patients with multiple sclerosis

Erdem ÇOKLUK; Aysel MİLANLIOĞLU; Zübeyir HUYUT; Vedat ÇİLİNGİR; Hamit Hakan ALP; Mehmet Nuri AYDIN; Mehmet Ramazan ŞEKEROĞLU; Ragıp BALAHOROĞLU

doi:https://doi.org/10.37212/jcnos.334360

Makale detayları

Levels of leukocyte oxidative DNA damage (8-OHdG), serum coenzyme Q10 and lipid peroxidation in the formation attacks of patients with multiple sclerosis

Yazarlar:

Erdem ÇOKLUK | Aysel MİLANLIOĞLU | Zübeyir HUYUT | Vedat ÇİLİNGİR | Hamit Hakan ALP | Mehmet Nuri AYDIN | Mehmet Ramazan ŞEKEROĞLU | Ragıp BALAHOROĞLU

Yayıncı:

Hücresel Sinir Bilimleri ve Oksidatif Stres Derneği

Dergi:

Journal of Cellular Neuroscience and Oxidative Stress 2017 Vol.9 Issue 1, pp.601-607

Belge Tipi:

Makale

Yayın Tarihi:

8/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000708918

Sıklık:

Üç Yıllık

eISSN:

2149-7222

DOI:

https://doi.org/10.37212/jcnos.334360

Özet:

 

Multiple sclerosis (MS) is a demyelinating disease
of the nervous system. Evidence about oxidative stress plays an important role
in the pathogenesis of MS is increasing day by day. In our study, we aimed to
investigate the effect of oxidative DNA damage and oxidative stress in the
pathogenesis of MS disease. Blood samples were obtained from during an attack
(Group 1), between attacks (Group 2) of MS patients (20 male and 10 female) and
30 healthy volunteers (Group 3). Malondialdehyde (MDA) levels as indicator of
oxidized lipids were detected using fluorescence dedector with high pressure
liquid chromatograph (HPLC). DNA was extracted from leukocytes of control and
patients with MS and then we measured 8-hydroxy- 2' -deoxyguanosine (8-OHdG) and deoxyguanosin (dG) by using HPLC method with electrochemical and
UV detector, respectively. Measurement of oxidized coenzyme Q10 (CoQ10) and
reduced CoQ (CoQ10H) was performed by using UV detector with HPLC method. Serum
MDA level of group 1 was significantly higher than those in group 2 and group 3
(p< 0.001). 8- OHdG/106 dG ratio
of group 1 was significantly higher than those in group 2 and group 3 (p<
0.001). CoQ10/CoQ10H rates of group 1 were significantly increased compared
with group 2 and group 3 (p<0.001). In conclusion, we observed that
oxidative DNA damage, lipid and mitochondria oxidative damage were high in
blood of patients with MS. It seems that oxidative stress acts a play role the
pathogenesis of MS patients as well as induces attacks.




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Makale detayları

Levels of leukocyte oxidative DNA damage (8-OHdG), serum coenzyme Q10 and lipid peroxidation in the formation attacks of patients with multiple sclerosis

Yazarlar: Erdem ÇOKLUK | Aysel MİLANLIOĞLU | Zübeyir HUYUT | Vedat ÇİLİNGİR | Hamit Hakan ALP | Mehmet Nuri AYDIN | Mehmet Ramazan ŞEKEROĞLU | Ragıp BALAHOROĞLU

Yayıncı: Hücresel Sinir Bilimleri ve Oksidatif Stres Derneği

Dergi: Journal of Cellular Neuroscience and Oxidative Stress 2017 Vol.9 Issue 1, pp.601-607

Belge Tipi: Makale

Yayın Tarihi: 8/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000708918

Sıklık: Üç Yıllık

eISSN: 2149-7222

DOI: https://doi.org/10.37212/jcnos.334360

Sayı

Yazarlar:

Erdem ÇOKLUK | Aysel MİLANLIOĞLU | Zübeyir HUYUT | Vedat ÇİLİNGİR | Hamit Hakan ALP | Mehmet Nuri AYDIN | Mehmet Ramazan ŞEKEROĞLU | Ragıp BALAHOROĞLU

Yayıncı:

Hücresel Sinir Bilimleri ve Oksidatif Stres Derneği

Dergi:

Journal of Cellular Neuroscience and Oxidative Stress 2017 Vol.9 Issue 1, pp.601-607

Belge Tipi:

Makale

Yayın Tarihi:

8/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000708918

Sıklık:

Üç Yıllık

eISSN:

2149-7222

DOI:

https://doi.org/10.37212/jcnos.334360