TRANSGALACTOSYLATION FOR GALACTOOLIGOSACCHARIDE SYNTHESIS USING PURIFIED AND CHARACTERIZED RECOMBINANT α-GALACTOSIDASE FROM Aspergillus fumigatus IMI 385708 OVEREXPRESSED IN Aspergillus sojae

Sümeyra GÜRKÖK; Zümrüt ÖGEL

doi:https://doi.org/10.3153/FH19007

Makale detayları

TRANSGALACTOSYLATION FOR GALACTOOLIGOSACCHARIDE SYNTHESIS USING PURIFIED AND CHARACTERIZED RECOMBINANT α-GALACTOSIDASE FROM Aspergillus fumigatus IMI 385708 OVEREXPRESSED IN Aspergillus sojae

Yazarlar:

Sümeyra GÜRKÖK | Zümrüt ÖGEL

Ana Başlıklar:

Gıda teknolojisi | Yaşam bilimleri

Dergi:

Food and Health 2019 Vol.5 Issue 1, pp.64-76

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000420000

DOI:

https://doi.org/10.3153/FH19007

Özet:

 


Galactooligosaccharides are well-known
functional food ingredients with prebiotic properties. Recent trend for the use
of galactooligosaccharides in the food industry leads the search for new
enzymes for their production. α-Galactosidase from Aspergillus fumigatus
IMI 385708, possessing a highly efficient debranching ability on polymeric
substrates, is also able to perform transgalactosylation. In this study,
recombinant α-galactosidase produced by Aspergillus sojae Ta1 was
purified 18.7-fold using anion exchange and hydrophobic interaction
chromatography with an overall yield of 56% and 64.7 U/mg protein specific
activity. The Vmax and Km values for the hydrolysis of pNPGal
were 78 U/mg protein and 0.45 mM, respectively. Optimum pH (pH 4.5) and
temperatures (50-60°C) for recombinant α-galactosidase activity were
determined. For the synthesis of oligosaccharides, purified and characterized
recombinant α-galactosidase was used in the transgalactosylation of various
mono- and disaccharides using pNPGal (p-nitrophenyl-α-D-galactopyranoside)
as galactose donor. Di- and trisaccharides obtained by transgalactosylation
were analysed by TLC, ESI-MS, and HPLC analysis. Among 12 acceptor candidates,
α-galactosidase transgalactosylated galactose, glucose, mannose, cellobiose,
lactose, maltose, and sucrose efficiently, however, did not transgalactosylate
xylose, arabinose, fucose, fructose, and melibiose.



.

Makale detayları

TRANSGALACTOSYLATION FOR GALACTOOLIGOSACCHARIDE SYNTHESIS USING PURIFIED AND CHARACTERIZED RECOMBINANT α-GALACTOSIDASE FROM Aspergillus fumigatus IMI 385708 OVEREXPRESSED IN Aspergillus sojae

Yazarlar: Sümeyra GÜRKÖK | Zümrüt ÖGEL

Ana Başlıklar: Gıda teknolojisi | Yaşam bilimleri

Dergi: Food and Health 2019 Vol.5 Issue 1, pp.64-76

Belge Tipi: Makale

Yayın Tarihi: 12/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000420000

DOI: https://doi.org/10.3153/FH19007

Sayı

Yazarlar:

Sümeyra GÜRKÖK | Zümrüt ÖGEL

Ana Başlıklar:

Gıda teknolojisi | Yaşam bilimleri

Dergi:

Food and Health 2019 Vol.5 Issue 1, pp.64-76

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000420000

DOI:

https://doi.org/10.3153/FH19007