Purification and characterization of mitochondrial thioredoxin reductase enzyme from rainbow trout (Oncorhynchus mykiss)  liver and investigation of the in vitro effects of some metal ions on the enzyme

İlknur ÖZGENÇLİ; Mehmet ÇİFTCİ

Makale detayları

Purification and characterization of mitochondrial thioredoxin reductase enzyme from rainbow trout (Oncorhynchus mykiss) liver and investigation of the in vitro effects of some metal ions on the enzyme

Yazarlar:

İlknur ÖZGENÇLİ | Mehmet ÇİFTCİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2016 Vol.40 Issue 1, pp.174-183

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000726705

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

Thioredoxin reductase (E.C 1.6.4.5.; TrxR) is an enzyme belonging to the flavoprotein family of pyridine nucleotide-disulfide oxidoreductases. In this study, mitochondrial TrxR enzyme was purified from rainbow trout mitochondria. Thanks to the 2 consecutive procedures (preparation of homogenate and 2',5'-ADP Sepharose 4B affinity chromatography), the enzyme, having the specific activity of 11.9 EU mg protein-1, was purified with a yield of 2.38{\%} and 672-fold. The purity of the enzyme was monitored and the molecular weight of its subunits was calculated as 70 kDa by SDS-PAGE. The native molecular mass of the enzyme was found to be approximately 151 kDa by gel filtration chromatography. Characteristic and kinetic properties of the enzyme were also determined. Furthermore, Se4+4+^{4+}, Cu2+2+^{2+}, Co2+2+^{2+}, Ni2+2+^{2+}, Fe3+3+^{3+}, and Al3+3+^{3+} metal ions' in vitro effects on mitochondrial TrxR purified from rainbow trout was investigated. While Se4+4+^{4+} ion increased the enzyme activity, all of the other metal ions used in this study showed an inhibitory effect.

Makale detayları

Purification and characterization of mitochondrial thioredoxin reductase enzyme from rainbow trout (Oncorhynchus mykiss) liver and investigation of the in vitro effects of some metal ions on the enzyme

Yazarlar: İlknur ÖZGENÇLİ | Mehmet ÇİFTCİ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2016 Vol.40 Issue 1, pp.174-183

Belge Tipi: Makale

Yayın Tarihi: 1/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000726705

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

İlknur ÖZGENÇLİ | Mehmet ÇİFTCİ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2016 Vol.40 Issue 1, pp.174-183

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000726705

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527